Abstract
In the establishment of symbiosis between Medicago truncatula and the nitrogen-fixing bacterium Sinorhizobium meliloti, the lipopolysaccharide (LPS) of the microsymbiont plays an important role as a signal molecule. It has been shown in cell cultures that the LPS is able to suppress an elicitor-induced oxidative burst. To investigate the effect of S. meliloti LPS on defense-associated gene expression, a microarray experiment was performed. For evaluation of the M. truncatula microarray datasets, the software tool MapMan, which was initially developed for the visualization of Arabidopsis (Arabidopsis thaliana) datasets, was adapted by assigning Medicago genes to the ontology originally created for Arabidopsis. This allowed functional visualization of gene expression of M. truncatula suspension-cultured cells treated with invertase as an elicitor. A gene expression pattern characteristic of a defense response was observed. Concomitant treatment of M. truncatula suspension-cultured cells with invertase and S. meliloti LPS leads to a lower level of induction of defense-associated genes compared to induction rates in cells treated with invertase alone. This suppression of defense-associated transcriptional rearrangement affects genes induced as well as repressed by elicitation and acts on transcripts connected to virtually all kinds of cellular processes. This indicates that LPS of the symbiont not only suppresses fast defense responses as the oxidative burst, but also exerts long-term influences, including transcriptional adjustment to pathogen attack. These data indicate a role for LPS during infection of the plant by its symbiotic partner.
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Claims 4
Claim · model-written summary
The adapted MapMan tool enabled functional visualization of elicited Medicago gene expression.
- Taxon
- M. truncatula
Medicago truncatulae0 - Treatment
- invertase as an elicitore0
- Outcome
- functional visualization of gene expressione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This allowed functional visualization of gene expression of M. truncatula suspension-cultured cells treated with invertase as an elicitor.”
Claim · model-written summary
Invertase elicitation produced a defense-response gene expression pattern.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Outcome
- gene expression pattern characteristic of a defense responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene expression pattern characteristic of a defense response was observed.”
t0“Medicago truncatula”
Claim · model-written summary
Concurrent S. meliloti LPS reduced induction of defense-associated genes relative to invertase alone.
- Taxon
- M. truncatulaMedicago truncatulae0
- Treatment
- invertase and S. meliloti LPSe0
- Interactor
- S. meliloti
Sinorhizobium melilotie0symbiont - Outcome
- induction of defense-associated genese0
- Direction
- lowere0
- Comparator
- cells treated with invertase alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concomitant treatment of M. truncatula suspension-cultured cells with invertase and S. meliloti LPS leads to a lower level of induction of defense-associated genes compared to induction rates in cells treated with invertase alone.”
Claim · model-written summary
LPS suppression extended to elicitation-induced and repressed genes across many cellular processes.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Outcome
- genes induced as well as repressed by elicitatione0
- Qualifier
- virtually all kinds of cellular processese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This suppression of defense-associated transcriptional rearrangement affects genes induced as well as repressed by elicitation and acts on transcripts connected to virtually all kinds of cellular processes.”
t0“Medicago truncatula”
The same questions in other papers
lipopolysaccharide · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
1 in the opposite direction
- no effect
Sinorhizobium meliloti · molecular: expression · Medicago truncatula (barrel medic)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.