Abstract
The plant hormone abscisic acid (ABA) has been suggested to play a role in fruit development, but supporting genetic evidence has been lacking. Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening. Using a newly established Tobacco rattle virus-induced gene silencing technique in strawberry fruit, the expression of a 9-cis-epoxycarotenoid dioxygenase gene (FaNCED1), which is key to ABA biosynthesis, was down-regulated, resulting in a significant decrease in ABA levels and uncolored fruits. Interestingly, a similar uncolored phenotype was observed in the transgenic RNA interference (RNAi) fruits, in which the expression of a putative ABA receptor gene encoding the magnesium chelatase H subunit (FaCHLH/ABAR) was down-regulated by virus-induced gene silencing. More importantly, the uncolored phenotype of the FaNCED1-down-regulated RNAi fruits could be rescued by exogenous ABA, but the ABA treatment could not reverse the uncolored phenotype of the FaCHLH/ABAR-down-regulated RNAi fruits. We observed that down-regulation of the FaCHLH/ABAR gene in the RNAi fruit altered both ABA levels and sugar content as well as a set of ABA- and/or sugar-responsive genes. Additionally, we showed that exogenous sugars, particularly sucrose, can significantly promote ripening while stimulating ABA accumulation. These data provide evidence that ABA is a signal molecule that promotes strawberry ripening and that the putative ABA receptor, FaCHLH/ABAR, is a positive regulator of ripening in response to ABA.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Silencing FaNCED1 decreased ABA levels in strawberry fruit.
- Taxon
- Fragaria ananassa
_single2.jpg?width=60)
Fragaria ×ananassae1 - Treatment
- Tobacco rattle virus-induced gene silencinge2
- Gene
- FaNCED1e2
- Compound
- ABAe2
- Outcome
- ABA levelse2
- Direction
- decreasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e2“Using a newly established Tobacco rattle virus-induced gene silencing technique in strawberry fruit, the expression of a 9-cis-epoxycarotenoid dioxygenase gene (FaNCED1), which is key to ABA biosynthesis, was down-regulated, resulting in a significant decrease in ABA levels and uncolored fruits”
Claim · model-written summary
Silencing FaNCED1 produced uncolored strawberry fruits.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- Tobacco rattle virus-induced gene silencinge2
- Gene
- FaNCED1e2
- Outcome
- uncolored fruitse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e2“Using a newly established Tobacco rattle virus-induced gene silencing technique in strawberry fruit, the expression of a 9-cis-epoxycarotenoid dioxygenase gene (FaNCED1), which is key to ABA biosynthesis, was down-regulated, resulting in a significant decrease in ABA levels and uncolored fruits”
Claim · model-written summary
Silencing FaCHLH/ABAR produced a similar uncolored phenotype in strawberry fruit.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- virus-induced gene silencinge3
- Gene
- FaCHLH/ABARe3
- Outcome
- uncolored phenotypee3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e3“a similar uncolored phenotype was observed in the transgenic RNA interference (RNAi) fruits, in which the expression of a putative ABA receptor gene encoding the magnesium chelatase H subunit (FaCHLH/ABAR) was down-regulated by virus-induced gene silencing”
Claim · model-written summary
Exogenous ABA rescued the uncolored phenotype of FaNCED1-silenced strawberry fruits.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- exogenous ABAe4
- Gene
- FaNCED1e4
- Compound
- ABAe4
- Outcome
- uncolored phenotypee4
- Direction
- could be rescuede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e4“the uncolored phenotype of the FaNCED1-down-regulated RNAi fruits could be rescued by exogenous ABA, but the ABA treatment could not reverse the uncolored phenotype of the FaCHLH/ABAR-down-regulated RNAi fruits”
Claim · model-written summary
ABA treatment could not reverse the uncolored phenotype of FaCHLH/ABAR-silenced strawberry fruits.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- ABA treatmente4
- Gene
- FaCHLH/ABARe4
- Outcome
- uncolored phenotypee4
- Direction
- could not reversee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e4“the uncolored phenotype of the FaNCED1-down-regulated RNAi fruits could be rescued by exogenous ABA, but the ABA treatment could not reverse the uncolored phenotype of the FaCHLH/ABAR-down-regulated RNAi fruits”
Claim · model-written summary
Down-regulation of FaCHLH/ABAR altered ABA levels and sugar content in strawberry fruit.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- down-regulation of the FaCHLH/ABAR genee5
- Gene
- FaCHLH/ABARe5
- Compound
- ABAe5
- Outcome
- ABA levels and sugar contente5
- Direction
- alterede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e5“down-regulation of the FaCHLH/ABAR gene in the RNAi fruit altered both ABA levels and sugar content as well as a set of ABA- and/or sugar-responsive genes”
Claim · model-written summary
Down-regulation of FaCHLH/ABAR altered ABA- and/or sugar-responsive gene expression in strawberry fruit.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- down-regulation of the FaCHLH/ABAR genee5
- Gene
- FaCHLH/ABARe5
- Outcome
- ABA- and/or sugar-responsive genese5
- Direction
- alterede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e5“down-regulation of the FaCHLH/ABAR gene in the RNAi fruit altered both ABA levels and sugar content as well as a set of ABA- and/or sugar-responsive genes”
Claim · model-written summary
Exogenous sugars, particularly sucrose, significantly promoted ripening in strawberry.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- exogenous sugars, particularly sucrosee6
- Outcome
- ripeninge6
- Direction
- promotee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e6“exogenous sugars, particularly sucrose, can significantly promote ripening while stimulating ABA accumulation”
Claim · model-written summary
Exogenous sugars, particularly sucrose, stimulated ABA accumulation in strawberry fruit.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Treatment
- exogenous sugars, particularly sucrosee6
- Compound
- ABAe6
- Outcome
- ABA accumulatione6
- Direction
- stimulatinge6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e6“exogenous sugars, particularly sucrose, can significantly promote ripening while stimulating ABA accumulation”
Claim · model-written summary
ABA is a signal molecule that promotes strawberry ripening.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Compound
- ABAe7
- Outcome
- strawberry ripeninge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e7“ABA is a signal molecule that promotes strawberry ripening and that the putative ABA receptor, FaCHLH/ABAR, is a positive regulator of ripening in response to ABA”
Claim · model-written summary
FaCHLH/ABAR is a positive regulator of strawberry ripening in response to ABA.
- Taxon
- Fragaria ananassaFragaria ×ananassae1
- Gene
- FaCHLH/ABARe7
- Outcome
- ripeninge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Here, we report that ABA promotes strawberry (Fragaria ananassa) fruit ripening”
e7“ABA is a signal molecule that promotes strawberry ripening and that the putative ABA receptor, FaCHLH/ABAR, is a positive regulator of ripening in response to ABA”
The same questions in other papers
sucrose · quality: postharvest · Fragaria x ananassa (strawberry)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.