Paper · 2010 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Corn and potato starch as an agar alternative for Solanum tuberosum micropropagation

M. Mohamed; AA Alsadon; Al Mohaidib

9
claims
18
verbatim quotes
30
citations
1
research areas
✓
Open access

Abstract

Potato single nodes were subcultured onto fresh MS medium gelled with 0, 1 and 2 g/l of agar + 40, 50 or 60 g/l of commercial corn and potato starch (CS or PS, respectively). After 4 weeks of culture, the pH of medium supplemented with 50 or 60 g/l of CS or 60 g/l of PS was significantly decreased to 3.91 - 4.00. This reduction coincided with a significant increment in electric conductivity (EC) which was 172 - 214 imhos/cm-1 for media containing 50 or 60 g/l of CS, or 60 g/l of PS. Corn and potato starch had no significant effect in plantlet height nevertheless, they significantly increased the number of shoots/explant over the control treatment (2.5) which had 7 g/l of agar. The highest number of shoots/explant (6.8) was achieved in medium with 50 or 60 g/l of PS + 1 g/l of agar. Plantlets developed on media with 40 g/l of CS or PS had higher shoot fresh and dry weight (p < 0.5) compared to those in the control one. Media with 50, 60 g/l of PS or 60 g/l of CS and 50 g/l of CS + agar at 1 g/l significantly enhanced the percentage of dry weight. Moreover, 92 - 98% of plantlets were acclimatized to the greenhouse conditions regardless the type of gelling agent. The results suggest that the combination of agar and PS or CS could offer a firm support for plant tissues and could be successfully used for potato micropropagation.

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Claims 9

Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Starch-supplemented media (50 or 60 g/l CS, or 60 g/l PS) decreased medium pH to 3.91-4.00 after 4 weeks.

Treatment
50 or 60 g/l of CS or 60 g/l of PSe1
Outcome
pH of mediume1
Direction
decreasede1
Value
3.91 - 4.00e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e1

“After 4 weeks of culture, the pH of medium supplemented with 50 or 60 g/l of CS or 60 g/l of PS was significantly decreased to 3.91 - 4.00”

✓ located verbatim in the source · abstract, characters 175–313 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Starch-supplemented media (50 or 60 g/l CS, or 60 g/l PS) increased electrical conductivity to 172-214 imhos/cm-1.

Treatment
50 or 60 g/l of CS, or 60 g/l of PSe2
Outcome
electric conductivity (EC)e2
Direction
incremente2
Value
172 - 214 imhos/cm-1e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e2

“This reduction coincided with a significant increment in electric conductivity (EC) which was 172 - 214 imhos/cm-1 for media containing 50 or 60 g/l of CS, or 60 g/l of PS”

✓ located verbatim in the source · abstract, characters 315–486 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Corn and potato starch had no significant effect on plantlet height.

Treatment
Corn and potato starche3
Outcome
plantlet heighte3
Direction
no significant effecte3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e3

“Corn and potato starch had no significant effect in plantlet height”

✓ located verbatim in the source · abstract, characters 488–555 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Corn and potato starch significantly increased the number of shoots per explant over the control treatment with 7 g/l agar.

Treatment
Corn and potato starche3
Outcome
number of shoots/explante4
Direction
increasede4
Qualifier
7 g/l of agare4
Comparator
control treatmente4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e3

“Corn and potato starch had no significant effect in plantlet height”

✓ located verbatim in the source · abstract, characters 488–555 · show in text ↑
e4

“they significantly increased the number of shoots/explant over the control treatment (2.5) which had 7 g/l of agar”

✓ located verbatim in the source · abstract, characters 570–684 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest number of shoots per explant (6.8) was obtained on 50 or 60 g/l PS with 1 g/l agar.

Treatment
50 or 60 g/l of PS + 1 g/l of agare5
Outcome
number of shoots/explante5
Direction
higheste5
Value
6.8e5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e5

“The highest number of shoots/explant (6.8) was achieved in medium with 50 or 60 g/l of PS + 1 g/l of agar”

✓ located verbatim in the source · abstract, characters 686–791 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Plantlets on 40 g/l CS or PS had higher shoot fresh and dry weight than those on the control.

Treatment
40 g/l of CS or PSe6
Outcome
shoot fresh and dry weighte6
Direction
highere6
Qualifier
p < 0.5e6
Comparator
control onee6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e6

“Plantlets developed on media with 40 g/l of CS or PS had higher shoot fresh and dry weight (p < 0.5) compared to those in the control one”

✓ located verbatim in the source · abstract, characters 793–930 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Media with 50, 60 g/l PS or 60 g/l CS, and 50 g/l CS + 1 g/l agar, significantly enhanced the percentage of dry weight.

Treatment
50, 60 g/l of PS or 60 g/l of CS and 50 g/l of CS + agar at 1 g/le7
Outcome
percentage of dry weighte7
Direction
enhancede7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e7

“Media with 50, 60 g/l of PS or 60 g/l of CS and 50 g/l of CS + agar at 1 g/l significantly enhanced the percentage of dry weight”

✓ located verbatim in the source · abstract, characters 932–1060 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

92-98% of plantlets were acclimatized to greenhouse conditions regardless of the gelling agent type.

Outcome
acclimatized to the greenhouse conditionse7
Value
92 - 98%e7
Qualifier
regardless the type of gelling agente7
Setting
greenhouse conditionse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Potato single nodes were subcultured onto fresh MS medium”

✓ located verbatim in the source · abstract, characters 0–57 · show in text ↑
e7

“92 - 98% of plantlets were acclimatized to the greenhouse conditions regardless the type of gelling agent”

✓ located verbatim in the source · abstract, characters 1072–1177 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The combination of agar and PS or CS is recommended as a firm support for potato micropropagation.

Outcome
the combination of agar and PS or CSe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e1

“the combination of agar and PS or CS could offer a firm support for plant tissues and could be successfully used for potato micropropagation”

✓ located verbatim in the source · abstract, characters 1204–1344 · show in text ↑

agar · growth · Solanum tuberosum (potato)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 reporting no difference from an alternative

  1. no effect
See all 2 papers →

Zea mays subsp. mays · growth · Solanum tuberosum (potato)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.