Abstract
The Arabidopsis bZIP transcription factor gene ATB2 has been shown previously to be expressed in a light-regulated and tissue-specific way. Here we describe the precise localization of ATB2 expression, using transgenic lines containing an ATB2 promoter-GUS reporter gene construct. The observed expression pattern suggests a role for ATB2 in the control of processes associated with the transport or utilization of metabolites. Remarkably, expression of the ATB2-GUS reporter gene construct was specifically repressed by sucrose. Other sugars, such as glucose and fructose, alone or in combination, were ineffective. Repression was observed at external sucrose concentrations exceeding 25 mM. Transcript levels of both the endogenous ATB2 gene and the ATB2-GUS reporter gene were not repressed by sucrose, suggesting that sucrose affects mRNA translation. This translational regulation involves the ATB2 leader sequence because deletion of the leader resulted in loss of sucrose repression. Our results provide evidence for a sucrose-specific sugar sensing and signalling system in plants.
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Claims 10
Claim · model-written summary
The ATB2 expression pattern suggests involvement in metabolite transport or utilization.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- ATB2e0
- Outcome
- transport or utilization of metabolitese0
- Qualifier
- observed expression patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The observed expression pattern suggests a role for ATB2 in the control of processes associated with the transport or utilization of metabolites.”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
Sucrose specifically repressed ATB2-GUS reporter expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- sucrosee0
- Gene
- ATB2-GUSe0
- Outcome
- expression of the ATB2-GUS reporter gene constructe0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of the ATB2-GUS reporter gene construct was specifically repressed by sucrose.”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
Glucose did not repress reporter expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- glucosee0
- Outcome
- ineffectivee0
- Direction
- ineffectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other sugars, such as glucose and fructose, alone or in combination, were ineffective.”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
Fructose did not repress reporter expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- fructosee0
- Outcome
- ineffectivee0
- Direction
- ineffectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other sugars, such as glucose and fructose, alone or in combination, were ineffective.”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
Repression occurred above 25 mM external sucrose.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- external sucrose concentrations exceeding 25 mMe0
- Outcome
- Repressione0
- Value
- 25 mMe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Repression was observed at external sucrose concentrations exceeding 25 mM.”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
Sucrose did not repress endogenous ATB2 transcript levels.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- sucrosee0
- Gene
- ATB2e0
- Outcome
- Transcript levels of both the endogenous ATB2 genee0
- Direction
- not repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of both the endogenous ATB2 gene and the ATB2-GUS reporter gene were not repressed by sucrose”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
Sucrose did not repress ATB2-GUS transcript levels.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- sucrosee0
- Gene
- ATB2-GUSe0
- Outcome
- Transcript levels of both the endogenous ATB2 gene and the ATB2-GUS reporter genee0
- Direction
- not repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of both the endogenous ATB2 gene and the ATB2-GUS reporter gene were not repressed by sucrose”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
The lack of transcript repression suggests that sucrose affects mRNA translation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- sucrosee0
- Outcome
- mRNA translatione0
- Qualifier
- suggestinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that sucrose affects mRNA translation.”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
Deleting the ATB2 leader eliminated sucrose repression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- deletion of the leadere0
- Gene
- ATB2t0
- Outcome
- sucrose repressione0
- Direction
- losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deletion of the leader resulted in loss of sucrose repression.”
t0“Arabidopsis ATB2 bZIP transcription factor gene”
Claim · model-written summary
The results support a sucrose-specific sugar sensing and signalling system in plants.
- Taxon
- plantsViridiplantaee0
- Treatment
- sucrosee0
- Outcome
- sugar sensing and signalling systeme0
Verbatim
e0“Our results provide evidence for a sucrose-specific sugar sensing and signalling system in plants.”
The same questions in other papers
sucrose · molecular: expression · ArabidopsisPositive and negative results
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