Abstract
Nitrate reductase, the first enzyme in nitrate assimilation, is located at the crossroad of two energy-consuming pathways: nitrate assimilation and carbon fixation. Light, which regulates the expression of many higher-plant carbon fixation genes, also regulates nitrate reductase gene expression. Located in the cytosol, nitrate reductase obtains its reductant not from photosynthesis but from carbohydrate catabolism. This relationship prompted us to investigate the indirect role that light might play, via photosynthesis, in the regulation of nitrate reductase gene expression. We show that sucrose can replace light in eliciting an increase of nitrate reductase mRNA accumulation in dark-adapted green Arabidopsis plants. We show further that sucrose alone is sufficient for the full expression of nitrate reductase genes in etiolated Arabidopsis plants. Finally, using a reporter gene, we show that a 2.7-kilobase region of 5' flanking sequence of the nitrate reductase gene is sufficient to confer the light or the sucrose response.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 3
Claim · model-written summary
Sucrose can replace light in eliciting an increase of nitrate reductase mRNA accumulation in dark-adapted green Arabidopsis plants.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- dark-adapted greene0
- Treatment
- sucrosee0
- Outcome
- nitrate reductase mRNA accumulatione0
- Direction
- increasee0
- Comparator
- lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose can replace light in eliciting an increase of nitrate reductase mRNA accumulation in dark-adapted green Arabidopsis plants”
Claim · model-written summary
Sucrose alone is sufficient for full expression of nitrate reductase genes in etiolated Arabidopsis plants.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- etiolatede0
- Treatment
- sucrose alonee0
- Outcome
- full expression of nitrate reductase genese0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose alone is sufficient for the full expression of nitrate reductase genes in etiolated Arabidopsis plants”
Claim · model-written summary
A 2.7-kilobase 5' flanking region of the nitrate reductase gene is sufficient to confer the light or sucrose response in a reporter gene assay.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- nitrate reductase genet0
- Outcome
- 2.7-kilobase region of 5' flanking sequencee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using a reporter gene, we show that a 2.7-kilobase region of 5' flanking sequence of the nitrate reductase gene is sufficient to confer the light or the sucrose response”
t0“Arabidopsis nitrate reductase gene”
The same questions in other papers
sucrose · molecular: expression · ArabidopsisPositive and negative results
11 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.