Abstract
Ectopic expression of a gibberellin 2-oxidase gene (PcGA2ox1) decreased the content of bioactive gibberellins (GAs) in transgenic wheat, producing a range of dwarf plants with different degrees of severity. In at least one case, a single transformation event gave rise to T(1) plants with different degrees of dwarfism, the phenotypes being stably inherited over at least four generations. The dwarf phenotype, which included dark-green leaves, increased tillering and, in severe cases, a prostrate growth habit, was replicated by the application of a GA biosynthesis inhibitor to the wild type. Ear rachis length, grain set, and grain size were also decreased in the wheat transformants, compared with an azygous (null) line. The extent of post-germination alpha-amylase production in grains reflected the severity of the shoot phenotype of the transformants and both developmental processes were restored to normal by the application of gibberellic acid (GA(3)). Expression of two GA biosynthesis genes (TaGA20ox1 and TaGA3ox2) was up-regulated, and that of two alpha-amylase gene families (alpha-Amy1 and alpha-Amy2) down regulated, in scutella of semi-dwarf lines, compared with controls. The marked decline in transcript abundance of both alpha-amylase gene families in aleurone was associated with a decreased content of bioactive GAs in grains of the semi-dwarf lines.
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Claims 13
Claim · model-written summary
PcGA2ox1 expression decreased bioactive gibberellin content.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- Ectopic expression of a gibberellin 2-oxidase gene (PcGA2ox1)e0
- Gene
- PcGA2ox1e0
- Outcome
- content of bioactive gibberellins (GAs)e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of a gibberellin 2-oxidase gene (PcGA2ox1) decreased the content of bioactive gibberellins (GAs) in transgenic wheat”
t0“wheat”
Claim · model-written summary
PcGA2ox1 expression produced dwarf plants of different severity.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- dwarf plantse0
- Qualifier
- different degrees of severitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“producing a range of dwarf plants with different degrees of severity”
t0“wheat”
Claim · model-written summary
Dwarf phenotypes from one transformation event were inherited for at least four generations.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- phenotypes being stably inheritede0
- Qualifier
- over at least four generationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a single transformation event gave rise to T(1) plants with different degrees of dwarfism, the phenotypes being stably inherited over at least four generations”
t0“wheat”
Claim · model-written summary
Dwarf plants had dark-green leaves and increased tillering.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- dark-green leaves, increased tilleringe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The dwarf phenotype, which included dark-green leaves, increased tillering and, in severe cases, a prostrate growth habit”
t0“wheat”
Claim · model-written summary
Severely dwarf plants had a prostrate growth habit.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- prostrate growth habite0
- Qualifier
- in severe casese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in severe cases, a prostrate growth habit”
t0“wheat”
Claim · model-written summary
A gibberellin biosynthesis inhibitor replicated the dwarf phenotype in wild type plants.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- application of a GA biosynthesis inhibitore0
- Outcome
- replicatede0
- Comparator
- wild typee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was replicated by the application of a GA biosynthesis inhibitor to the wild type”
t0“wheat”
Claim · model-written summary
Transformants had shorter ear rachises than an azygous line.
- Taxon
- wheatTriticum aestivumt0
- Material
- wheat transformantse0
- Outcome
- Ear rachis lengthe0
- Direction
- decreasede0
- Comparator
- azygous (null) linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ear rachis length, grain set, and grain size were also decreased in the wheat transformants, compared with an azygous (null) line”
t0“wheat”
Claim · model-written summary
Transformants had reduced grain set and size relative to an azygous line.
- Taxon
- wheatTriticum aestivumt0
- Material
- wheat transformantse0
- Outcome
- grain set, and grain sizee0
- Direction
- decreasede0
- Comparator
- azygous (null) linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ear rachis length, grain set, and grain size were also decreased in the wheat transformants, compared with an azygous (null) line”
t0“wheat”
Claim · model-written summary
Post-germination alpha-amylase production reflected shoot phenotype severity.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- grainse0
- Gene
- alpha-amylasee0
- Outcome
- post-germination alpha-amylase productione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extent of post-germination alpha-amylase production in grains reflected the severity of the shoot phenotype of the transformants”
t0“wheat”
Claim · model-written summary
Gibberellic acid restored alpha-amylase production and shoot development to normal.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- application of gibberellic acid (GA(3))e0
- Outcome
- developmental processese0
- Direction
- restored to normale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both developmental processes were restored to normal by the application of gibberellic acid (GA(3))”
t0“wheat”
Claim · model-written summary
TaGA20ox1 and TaGA3ox2 expression was up-regulated in semi-dwarf scutella.
- Taxon
- wheatTriticum aestivumt0
- Material
- semi-dwarf linese0
- Organ / stage
- scutellae0
- Gene
- TaGA20ox1 and TaGA3ox2e0
- Outcome
- Expressione0
- Direction
- up-regulatede0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of two GA biosynthesis genes (TaGA20ox1 and TaGA3ox2) was up-regulated, and that of two alpha-amylase gene families (alpha-Amy1 and alpha-Amy2) down regulated, in scutella of semi-dwarf lines, compared with controls”
t0“wheat”
Claim · model-written summary
Alpha-Amy1 and alpha-Amy2 expression was down regulated in semi-dwarf scutella.
- Taxon
- wheatTriticum aestivumt0
- Material
- semi-dwarf linese0
- Organ / stage
- scutellae0
- Gene
- alpha-Amy1 and alpha-Amy2e0
- Outcome
- Expressione0
- Direction
- down regulatede0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of two GA biosynthesis genes (TaGA20ox1 and TaGA3ox2) was up-regulated, and that of two alpha-amylase gene families (alpha-Amy1 and alpha-Amy2) down regulated, in scutella of semi-dwarf lines, compared with controls”
t0“wheat”
Claim · model-written summary
Lower alpha-amylase transcript abundance in aleurone was associated with reduced grain bioactive gibberellins.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- aleuronee0
- Gene
- alpha-amylase gene familiese0
- Compound
- bioactive GAse0
- Outcome
- transcript abundancee0
- Direction
- marked declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The marked decline in transcript abundance of both alpha-amylase gene families in aleurone was associated with a decreased content of bioactive GAs in grains of the semi-dwarf lines”
t0“wheat”
The same questions in other papers
class · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.