Abstract
The polypeptide composition of the membranes from corn (Zea mays L.) seedling roots upon nitrate induction was determined by two-dimensional gel electrophoresis and silver-staining. The synthesis of five polypeptides (49, 48, 35, 33, and 32 kDa) in the tono-plast fraction and four polypeptides (50, 49, 38, and 33 kDa) in the plasma membrane fraction was induced by both 2.5 mM Ca(NO3)2 and 5 mM KNO3. Extensive washing of the membranes with salt and NaOH demonstrated that three induced polypeptides (49, 48, and 35 kDa) in the tonoplast fraction and two induced polypeptides (49 and 33 kDa) in the plasma membrane fraction were integral proteins. After incubation of seedlings in N-free medium for 4 d, the 49 and 32 kDa polypeptides in the tonoplast fraction had disappeared. By the sixth day in N-free medium, the 35 kDa polypeptide had disappeared from the tonoplast fraction. The 50 kDa polypeptide of the plasma membrane fraction was no longer detectable in seedlings incubated for 6 d in N-free medium. The size of the spots corresponding to the 33 kDa polypeptides of both membrane fractions and to the 49 kDa polypeptide of the plasma membrane fraction was reduced following incubation of seedlings in N-free medium. The changes in nitrate-induced polypeptides in both membrane fractions following transfer to N-free medium correlated with a reduced capacity to take up nitrate in the treated seedlings. The results support the conclusion that the nitrate-induced polypeptides may be involved in nitrate transport across the tonoplast and plasma membrane.
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Claims 10
Claim · model-written summary
Both nitrate salts induced five tonoplast polypeptides.
- Taxon
- corn
Zea mays subsp. mayst0 - Organ / stage
- seedling rootst0
- Treatment
- 2.5 mM Ca(NO3)2 and 5 mM KNO3e0
- Outcome
- five polypeptides (49, 48, 35, 33, and 32 kDa) in the tono-plast fractione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The synthesis of five polypeptides (49, 48, 35, 33, and 32 kDa) in the tono-plast fraction and four polypeptides (50, 49, 38, and 33 kDa) in the plasma membrane fraction was induced by both 2.5 mM Ca(NO3)2 and 5 mM KNO3”
t0“corn seedling roots”
Claim · model-written summary
Both nitrate salts induced four plasma membrane polypeptides.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- 2.5 mM Ca(NO3)2 and 5 mM KNO3e0
- Outcome
- four polypeptides (50, 49, 38, and 33 kDa) in the plasma membrane fractione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four polypeptides (50, 49, 38, and 33 kDa) in the plasma membrane fraction was induced by both 2.5 mM Ca(NO3)2 and 5 mM KNO3”
t0“corn seedling roots”
Claim · model-written summary
Three induced tonoplast polypeptides were integral proteins.
- Taxon
- cornZea mays subsp. mayst0
- Outcome
- three induced polypeptides (49, 48, and 35 kDa) in the tonoplast fractione0
- Qualifier
- integral proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three induced polypeptides (49, 48, and 35 kDa) in the tonoplast fraction and two induced polypeptides (49 and 33 kDa) in the plasma membrane fraction were integral proteins”
t0“corn seedling roots”
Claim · model-written summary
Two induced plasma membrane polypeptides were integral proteins.
- Taxon
- cornZea mays subsp. mayst0
- Outcome
- two induced polypeptides (49 and 33 kDa) in the plasma membrane fractione0
- Qualifier
- integral proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two induced polypeptides (49 and 33 kDa) in the plasma membrane fraction were integral proteins”
t0“corn seedling roots”
Claim · model-written summary
After 4 days in N-free medium, two tonoplast polypeptides disappeared.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- N-free medium for 4 de0
- Outcome
- 49 and 32 kDa polypeptides in the tonoplast fractione0
- Direction
- disappearede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After incubation of seedlings in N-free medium for 4 d, the 49 and 32 kDa polypeptides in the tonoplast fraction had disappeared”
t0“corn seedling roots”
Claim · model-written summary
After six days in N-free medium, the 35 kDa tonoplast polypeptide disappeared.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- N-free mediume0
- Outcome
- 35 kDa polypeptidee0
- Direction
- disappearede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By the sixth day in N-free medium, the 35 kDa polypeptide had disappeared from the tonoplast fraction”
t0“corn seedling roots”
Claim · model-written summary
After six days in N-free medium, the 50 kDa plasma membrane polypeptide was undetectable.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- N-free mediume0
- Outcome
- 50 kDa polypeptide of the plasma membrane fractione0
- Direction
- no longer detectablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 50 kDa polypeptide of the plasma membrane fraction was no longer detectable in seedlings incubated for 6 d in N-free medium”
t0“corn seedling roots”
Claim · model-written summary
N-free medium reduced the spots for 33 kDa polypeptides in both fractions and 49 kDa in plasma membrane.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- N-free mediume0
- Outcome
- size of the spots corresponding to the 33 kDa polypeptides of both membrane fractions and to the 49 kDa polypeptide of the plasma membrane fractione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The size of the spots corresponding to the 33 kDa polypeptides of both membrane fractions and to the 49 kDa polypeptide of the plasma membrane fraction was reduced following incubation of seedlings in N-free medium”
t0“corn seedling roots”
Claim · model-written summary
Membrane polypeptide changes after N withdrawal correlated with reduced nitrate uptake capacity.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- transfer to N-free mediume0
- Outcome
- capacity to take up nitratee0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The changes in nitrate-induced polypeptides in both membrane fractions following transfer to N-free medium correlated with a reduced capacity to take up nitrate in the treated seedlings”
t0“corn seedling roots”
Claim · model-written summary
The authors infer nitrate-induced polypeptides may participate in nitrate transport.
- Taxon
- cornZea mays subsp. mayst0
- Gene
- nitrate-induced polypeptidese0
- Outcome
- nitrate transport across the tonoplast and plasma membranee0
- Qualifier
- may be involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrate-induced polypeptides may be involved in nitrate transport across the tonoplast and plasma membrane”
t0“corn seedling roots”
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See all 87 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.