Abstract
Tomato bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis, is transmitted by infected or infested seed and mechanically from plant to plant. Wounds occurring during seedling production and crop maintenance facilitate the dissemination of the pathogen. However, the effects of environmental factors on C. michiganensis subsp. michiganensis translocation and growth as an endophyte have not been fully elucidated. A virulent, stable, constitutively bioluminescent C. michiganensis subsp. michiganensis strain BL-Cmm 17 coupled with an in vivo imaging system allowed visualization of the C. michiganensis subsp. michiganensis colonization process in tomato seedlings in real time. The dynamics of bacterial infection in seedlings through wounds were compared under low (45%) and high (83%) relative humidity. Bacteria multiplied rapidly in cotyledon petioles remaining after clip inoculation and moved in the stem toward both root and shoot. Luminescent signals were also observed in tomato seedling roots over time, and root development was reduced in inoculated plants maintained under both humidity regimes. Wilting was more severe in seedlings under high-humidity regimes. A strong positive correlation between light intensity and bacterial population in planta suggests that bioluminescent C. michiganensis subsp. michiganensis strains will be useful in evaluating the efficacy of bactericides and host resistance.
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Claims 6
Claim · model-written summary
The bioluminescent strain and imaging system visualized colonization in tomato seedlings in real time.
- Taxon
- tomato
Solanum lycopersicume0 - Interactor
- C. michiganensis subsp. michiganensis
Clavibacter michiganensis subsp. michiganensise0pathogen - Outcome
- visualization of the C. michiganensis subsp. michiganensis colonization processe0
- Value
- real timee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A virulent, stable, constitutively bioluminescent C. michiganensis subsp. michiganensis strain BL-Cmm 17 coupled with an in vivo imaging system allowed visualization of the C. michiganensis subsp. michiganensis colonization process in tomato seedlings in real time.”
Claim · model-written summary
Bacteria multiplied in remaining cotyledon petioles and moved toward roots and shoots.
- Taxon
- TomatoSolanum lycopersicumt0
- Interactor
- Clavibacter michiganensis subsp. michiganensisClavibacter michiganensis subsp. michiganensist0pathogen
- Outcome
- Bacteria multiplied rapidly in cotyledon petioles remaining after clip inoculation and moved in the stem toward both root and shoote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacteria multiplied rapidly in cotyledon petioles remaining after clip inoculation and moved in the stem toward both root and shoot.”
t0“Tomato Seedlings by Bioluminescent Clavibacter michiganensis subsp. michiganensis”
Claim · model-written summary
Luminescent signals appeared in tomato seedling roots over time.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- seedling rootse0
- Interactor
- Clavibacter michiganensis subsp. michiganensisClavibacter michiganensis subsp. michiganensist0pathogen
- Outcome
- Luminescent signalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Luminescent signals were also observed in tomato seedling roots over time”
t0“Clavibacter michiganensis subsp. michiganensis”
Claim · model-written summary
Inoculation reduced root development under both humidity regimes.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- inoculatede0
- Interactor
- Clavibacter michiganensis subsp. michiganensisClavibacter michiganensis subsp. michiganensist0pathogen
- Outcome
- root developmente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root development was reduced in inoculated plants maintained under both humidity regimes.”
t0“Tomato Seedlings by Bioluminescent Clavibacter michiganensis subsp. michiganensis”
Claim · model-written summary
Wilting was more severe under high humidity.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- high-humidity regimese0
- Interactor
- Clavibacter michiganensis subsp. michiganensisClavibacter michiganensis subsp. michiganensist0pathogen
- Outcome
- Wiltinge0
- Direction
- more severee0
- Comparator
- low (45%)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wilting was more severe in seedlings under high-humidity regimes.”
e1“low (45%) and high (83%) relative humidity”
t0“Tomato Seedlings by Bioluminescent Clavibacter michiganensis subsp. michiganensis”
Claim · model-written summary
Light intensity correlated positively with bacterial population in planta.
- Taxon
- TomatoSolanum lycopersicumt0
- Interactor
- C. michiganensis subsp. michiganensisClavibacter michiganensis subsp. michiganensise1pathogen
- Outcome
- bacterial population in plantae0
- Direction
- positivee0
- Comparator
- light intensitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A strong positive correlation between light intensity and bacterial population in planta”
e1“bioluminescent C. michiganensis subsp. michiganensis strains”
t0“Tomato”
The same questions in other papers
inoculation · Clavibacter michiganensis subsp. michiganensis · Solanum lycopersicum (tomato)Positive and negative results
4 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.