Abstract
The binding protein (BiP) is an important component of endoplasmic reticulum stress response of cells. Despite extensive studies in cultured cells, a protective function of BiP against stress has not yet been demonstrated in whole multicellular organisms. Here, we have obtained transgenic tobacco (Nicotiana tabacum L. cv Havana) plants constitutively expressing elevated levels of BiP or its antisense cDNA to analyze the protective role of this endoplasmic reticulum lumenal stress protein at the whole plant level. Elevated levels of BiP in transgenic sense lines conferred tolerance to the glycosylation inhibitor tunicamycin during germination and tolerance to water deficit during plant growth. Under progressive drought, the leaf BiP levels correlated with the maintenance of the shoot turgidity and water content. The protective effect of BiP overexpression against water stress was disrupted by expression of an antisense BiP cDNA construct. Although overexpression of BiP prevented cellular dehydration, the stomatal conductance and transpiration rate in droughted sense leaves were higher than in control and antisense leaves. The rate of photosynthesis under water deficit might have caused a degree of greater osmotic adjustment in sense leaves because it remained unaffected during water deprivation, which was in marked contrast with the severe drought-induced decrease in the CO(2) assimilation in control and antisense leaves. In antisense plants, the water stress stimulation of the antioxidative defenses was higher than in control plants, whereas in droughted sense leaves an induction of superoxide dismutase activity was not observed. These results suggest that overexpression of BiP in plants may prevent endogenous oxidative stress.
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Claims 10
Claim · model-written summary
Elevated BiP expression increased germination tolerance to tunicamycin.
- Taxon
- tobacco
Nicotiana tabacume1 - Material
- transgenic sense linese0
- Treatment
- tunicamycine0
- Gene
- BiPe0
- Outcome
- tolerance to the glycosylation inhibitor tunicamycin during germinatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated levels of BiP in transgenic sense lines conferred tolerance to the glycosylation inhibitor tunicamycin during germination”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
Elevated BiP expression increased tolerance to water deficit during growth.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- water deficite0
- Gene
- BiPe0
- Outcome
- tolerance to water deficit during plant growthe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated levels of BiP in transgenic sense lines conferred tolerance to the glycosylation inhibitor tunicamycin during germination and tolerance to water deficit during plant growth.”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
Under progressive drought, leaf BiP levels correlated with shoot turgidity and water content maintenance.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- progressive droughte0
- Gene
- BiPe0
- Outcome
- maintenance of the shoot turgidity and water contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under progressive drought, the leaf BiP levels correlated with the maintenance of the shoot turgidity and water content.”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
Antisense BiP expression disrupted the protective effect of BiP overexpression against water stress.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- expression of an antisense BiP cDNA constructe0
- Gene
- BiPe0
- Outcome
- protective effect of BiP overexpression against water stresse0
- Direction
- disruptede0
- Comparator
- BiP overexpressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protective effect of BiP overexpression against water stress was disrupted by expression of an antisense BiP cDNA construct.”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
BiP overexpression prevented cellular dehydration.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overexpression of BiPe0
- Gene
- BiPe0
- Outcome
- cellular dehydratione0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of BiP prevented cellular dehydration”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
Droughted BiP sense leaves had higher stomatal conductance and transpiration than control and antisense leaves.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- droughted sense leavese0
- Outcome
- stomatal conductance and transpiration ratee0
- Direction
- highere0
- Comparator
- control and antisense leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the stomatal conductance and transpiration rate in droughted sense leaves were higher than in control and antisense leaves.”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
Photosynthesis remained unaffected during water deprivation in sense leaves, contrasting with severe CO2 assimilation decreases in control and antisense leaves.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- water deprivatione0
- Outcome
- rate of photosynthesise1
- Direction
- unaffectede0
- Comparator
- control and antisense leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it remained unaffected during water deprivation, which was in marked contrast with the severe drought-induced decrease in the CO(2) assimilation in control and antisense leaves.”
e1“The rate of photosynthesis under water deficit”
e2“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
Water stress stimulated antioxidative defenses more in antisense than control plants.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- water stresse0
- Outcome
- antioxidative defensese0
- Direction
- highere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In antisense plants, the water stress stimulation of the antioxidative defenses was higher than in control plants”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
Droughted sense leaves did not show induction of superoxide dismutase activity.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- superoxide dismutasee0
- Outcome
- induction of superoxide dismutase activity was not observede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in droughted sense leaves an induction of superoxide dismutase activity was not observed.”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
Claim · model-written summary
The authors suggest BiP overexpression may prevent endogenous oxidative stress.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overexpression of BiPe0
- Gene
- BiPe0
- Outcome
- endogenous oxidative stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that overexpression of BiP in plants may prevent endogenous oxidative stress.”
e1“transgenic tobacco (Nicotiana tabacum L. cv Havana) plants”
The same questions in other papers
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drought · physiology: gas exchange · Nicotiana tabacum (tobacco)Positive and negative results
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8 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- negative
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.