Abstract
Some factors related to cessation of the movement of tobacco mosaic tobamovirus (TMV) in the late phase of a defence response were examined. Mesophyll cells surrounding the local lesions induced by TMV in N. tabacum cv. Xanthi-nc were micro-injected with fluorescent dye 2, 3 and 7 days post-inoculation. At 7 days post-inoculation, twelve out of 20 injections into cells adjacent to the lesion, showed the expected dye-coupling (outflow of fluorescent dye from injected cell to adjacent ones via plasmodesmata) whereas 17–20 out of 20 injections were successful in other cases. Callose inhibitor (tunicamycin), dark treatment and incubation of plants with ascorbic acid, which play a role in blocking plasmodesmata or induction of defence responses, did not seem to have an effect on lesion growth. These data imply that defects in the plasmodesmal function, although not total, may account for the formation of late local defence reaction together with other factors that restrict viral spread in the continuous cell-to-cell mode in mesophyll tissue. Zusammenfassung Untersucht wurden einige Faktoren, die mit dem Stop der Bewegung des Tabakmosaik-Tobamovirus (TMV) in der spaten Phase einer Abwehrreaktion in Verbindung stehen. Mesophyllzellen, die die Lokallasionen umgeben, welche durch TMV bei N. tabacum cv. Xanthi-nc hervorgerufen worden waren, erhielten 2, 3 und 7 Tage nach der Inokulation (d p.i.) Mikroinjektionen mit fluoreszierendem Farbstoff. Zwolf von 20 am 7. d p.i. ausgefuhrten Injektionen in Zellen neben Lasionen bewirkten die erwartete Farbstoffbindung (Ausfliesen fluoreszierenden Farbstoffs von injizierten Zellen in die benachbarten Zellen durch Plasmodesmen). In anderen Fallen waren 17 bis 20 von 20 Infektionen erfolgreich. Der Kallosehemmer Tunicamycin, Dunkelheit und eine Inkubation der Pflanzen mit Ascorbinsaure, die eine Rolle bei der Blockierung von Plasmodesmen oder der Induktion von Abwehrreaktionen spielt, hatten keinen feststellbaren Einflus auf das Wachstum der Lasionen. Diese Ergebnisse zeigen, das Defekte in der Funktion der Plasmodesmen gemeinsam mit anderen Faktoren, die die kontinuierliche Ausbreitung des Virus von Zelle zu Zelle in Mesophyllgewebe begrenzen, fur die Auslosung einer spaten lokalen Abwehrreaktion verantwortlich sein konnen. Dies gilt auch dann, wenn die Funktion der Plasmodesmen nicht vollstandig zum Erliegen kommt.
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Claims 5
Claim · model-written summary
Dye coupling occurred in 12 of 20 cells adjacent to lesions at day seven versus 17 to 20 of 20 in other cases.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Organ / stage
- cells adjacent to the lesione0
- Outcome
- dye-couplinge0
- Value
- twelve out of 20e0
- Qualifier
- 17–20 out of 20e0
- Comparator
- other casese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 7 days post-inoculation, twelve out of 20 injections into cells adjacent to the lesion, showed the expected dye-coupling (outflow of fluorescent dye from injected cell to adjacent ones via plasmodesmata) whereas 17–20 out of 20 injections were successful in other cases.”
t0“Tobacco Mesophyll Cells Surrounding Growing Tobacco Mosaic Tobamovirus-induced Local Lesions”
Claim · model-written summary
Tunicamycin did not appear to affect tobacco lesion growth.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- tunicamycine0
- Outcome
- lesion growthe0
- Direction
- did not seem to have an effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callose inhibitor (tunicamycin), dark treatment and incubation of plants with ascorbic acid, which play a role in blocking plasmodesmata or induction of defence responses, did not seem to have an effect on lesion growth.”
t0“Tobacco Mesophyll Cells Surrounding Growing Tobacco Mosaic Tobamovirus-induced Local Lesions”
Claim · model-written summary
Dark treatment did not appear to affect tobacco lesion growth.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- dark treatmente0
- Outcome
- lesion growthe0
- Direction
- did not seem to have an effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callose inhibitor (tunicamycin), dark treatment and incubation of plants with ascorbic acid, which play a role in blocking plasmodesmata or induction of defence responses, did not seem to have an effect on lesion growth.”
t0“Tobacco Mesophyll Cells Surrounding Growing Tobacco Mosaic Tobamovirus-induced Local Lesions”
Claim · model-written summary
Ascorbic acid incubation did not appear to affect tobacco lesion growth.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- incubation of plants with ascorbic acide0
- Outcome
- lesion growthe0
- Direction
- did not seem to have an effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callose inhibitor (tunicamycin), dark treatment and incubation of plants with ascorbic acid, which play a role in blocking plasmodesmata or induction of defence responses, did not seem to have an effect on lesion growth.”
t0“Tobacco Mesophyll Cells Surrounding Growing Tobacco Mosaic Tobamovirus-induced Local Lesions”
Claim · model-written summary
The authors infer that partial plasmodesmal defects may contribute to late local defense and restricted viral spread.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- formation of late local defence reactione0
- Qualifier
- defects in the plasmodesmal function, although not totale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“defects in the plasmodesmal function, although not total, may account for the formation of late local defence reaction together with other factors that restrict viral spread in the continuous cell-to-cell mode in mesophyll tissue.”
t0“Tobacco Mesophyll Cells Surrounding Growing Tobacco Mosaic Tobamovirus-induced Local Lesions”
The same questions in other papers
tunicamycin · disease · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- positive
ascorbic acid · disease · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- negative
light · disease · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.