Abstract
Salicylic acid (SA) plays a key role in plant resistance to pathogens. Accumulation of SA is induced by wounding in tobacco plants in which the expression of WIPK and SIPK, two mitogen-activated protein kinases, is suppressed. Here, the mechanisms underlying the abnormal accumulation of SA in WIPK/SIPK-suppressed plants have been characterized. SA accumulation started around 12 h after wounding and was inhibited by cycloheximide (CHX), a protein synthesis inhibitor. SA accumulation, however, was enhanced several fold when leaf discs were transferred onto CHX after floating on water for ≥6 h. Temporal and spatial analyses of wound-induced and CHX-enhanced SA accumulation suggested that wounding induces activators for SA accumulation followed by the generation of repressors, and late CHX treatment inhibits the production of repressors more efficiently than that of activators. Microarray analysis revealed that the expression of many disease resistance-related genes, including N, a Resistance (R) gene for Tobacco mosaic virus and R gene-like genes, was up-regulated in wounded WIPK/SIPK-suppressed plants. Expression of the N gene and R gene-like genes peaked earlier than that of most other genes as well as SA accumulation, and was mainly induced in those parts of leaf discs where SA was highly accumulated. Moreover, wound-induced SA accumulation was decreased by the treatments which compromise the function of R proteins. These results indicate that signaling leading to the expression of disease resistance-related genes is activated by wounding in WIPK/SIPK-suppressed plants, and induction of R gene and R gene-like genes might lead to the biosynthesis of SA.
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Claims 9
Claim · model-written summary
Salicylic acid accumulation began about 12 hours after wounding in suppressed tobacco plants.
- Taxon
- tobacco plants
Nicotiana tabacume1 - Treatment
- woundinge0
- Outcome
- SA accumulatione0
- Value
- around 12 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA accumulation started around 12 h after wounding”
e1“tobacco plants”
Claim · model-written summary
Cycloheximide inhibited wound-induced SA accumulation.
- Taxon
- tobacco plantsNicotiana tabacume1
- Treatment
- cycloheximide (CHX)e0
- Outcome
- SA accumulatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA accumulation started around 12 h after wounding and was inhibited by cycloheximide (CHX)”
e1“tobacco plants”
Claim · model-written summary
Late transfer to cycloheximide after at least six hours on water enhanced SA accumulation several fold.
- Taxon
- tobacco plantsNicotiana tabacume1
- Treatment
- transferred onto CHXe0
- Outcome
- SA accumulatione0
- Direction
- enhancede0
- Value
- several folde0
- Qualifier
- after floating on water for ≥6 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA accumulation, however, was enhanced several fold when leaf discs were transferred onto CHX after floating on water for ≥6 h”
e1“tobacco plants”
Claim · model-written summary
Wounding up-regulated many disease resistance-related genes in suppressed plants.
- Taxon
- tobacco plantsNicotiana tabacume1
- Treatment
- woundede0
- Gene
- Ne0
- Outcome
- expression of many disease resistance-related genese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of many disease resistance-related genes, including N, a Resistance (R) gene for Tobacco mosaic virus and R gene-like genes, was up-regulated in wounded WIPK/SIPK-suppressed plants”
e1“tobacco plants”
Claim · model-written summary
N and R gene-like gene expression peaked before most other genes and SA accumulation.
- Taxon
- tobacco plantsNicotiana tabacume1
- Gene
- N gene and R gene-like genese0
- Outcome
- Expressione0
- Direction
- peaked earliere0
- Comparator
- most other genes as well as SA accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the N gene and R gene-like genes peaked earlier than that of most other genes as well as SA accumulation”
e1“tobacco plants”
Claim · model-written summary
N and R gene-like genes were chiefly induced in leaf disc regions with high SA accumulation.
- Taxon
- tobacco plantsNicotiana tabacume1
- Organ / stage
- leaf discse0
- Outcome
- was mainly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was mainly induced in those parts of leaf discs where SA was highly accumulated”
e1“tobacco plants”
Claim · model-written summary
Treatments compromising R protein function decreased wound-induced SA accumulation.
- Taxon
- tobacco plantsNicotiana tabacume1
- Treatment
- treatments which compromise the function of R proteinse0
- Outcome
- wound-induced SA accumulatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wound-induced SA accumulation was decreased by the treatments which compromise the function of R proteins”
e1“tobacco plants”
Claim · model-written summary
The authors infer that wounding activates signalling to disease resistance gene expression.
- Taxon
- tobacco plantsNicotiana tabacume1
- Treatment
- woundinge0
- Outcome
- expression of disease resistance-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“signaling leading to the expression of disease resistance-related genes is activated by wounding in WIPK/SIPK-suppressed plants”
e1“tobacco plants”
Claim · model-written summary
The authors propose R and R-like gene induction may lead to SA biosynthesis.
- Taxon
- tobacco plantsNicotiana tabacume1
- Gene
- R gene and R gene-like genese0
- Outcome
- biosynthesis of SAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induction of R gene and R gene-like genes might lead to the biosynthesis of SA”
e1“tobacco plants”
The same questions in other papers
cycloheximide · physiology: hormones · Nicotiana tabacum (tobacco)Positive and negative results
2 in the opposite direction
- no effect
- negative
mechanical · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
16 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.