Abstract
Species of the genus Monilinia are important causal agents of fruit rot on pome and stone fruits in Serbia. The pathogen is very harmful, especially on small properties and cottage plantations where intensive control measures are not applied. Quince is important host for the pathogens of this genus. During spring 2010, intensive occurrence of mummified fruits overwintering on branches of the quince was observed. The pathogen was isolated using standard phytopathological methods. Pathogenicity of eight obtained isolates was tested by artificial inoculation of injured apple fruits. Identification was performed according to pathogenic, morphological and ecological properties, and was confirmed by Multiplex polimeraze chain reaction, PCR. All the isolates studied caused brown rot on inoculated apple fruits. The isolates form light yellow colonies with lobate margins, with single-celled, transparent, elliptical or oval conidia in chains, regardless temperature or light presence. Sclerotia are observed in 14 days old cultures. The highest growth rate of most of the isolates is at 27?C and in dark. Based on studied pathogenic, morphological and ecological characteristics, it was found that the Monilinia fructigena is causal agent of brown rot of quince. Using specific primers (MO368-5, MO368-8R, MO368-10R, Laxa-R2) for detection of Monilinia species in Multiplex PCR reaction, the expected fragment 402 bp in size was amplified, which confirmed that the studied isolates belonged to the species M. fructigena.
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Claims 7
Claim · model-written summary
Mummified fruits overwintering on quince branches occurred intensively in spring 2010.
- Taxon
- quince
Cydonia oblongae0 - Organ / stage
- fruitse0
- Outcome
- intensive occurrence of mummified fruitse0
- Qualifier
- During spring 2010e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During spring 2010, intensive occurrence of mummified fruits overwintering on branches of the quince was observed”
Claim · model-written summary
All tested isolates caused brown rot on inoculated apple fruits.
- Taxon
- apple
Malus domesticae0 - Organ / stage
- fruitse0
- Treatment
- inoculatede0
- Outcome
- brown rote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the isolates studied caused brown rot on inoculated apple fruits”
Claim · model-written summary
The isolates formed light yellow colonies with lobate margins and single-celled transparent elliptical or oval conidia in chains.
- Taxon
- Monilinia fructigena
Monilinia fructigenae1 - Outcome
- light yellow colonies with lobate margins, with single-celled, transparent, elliptical or oval conidia in chainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The isolates form light yellow colonies with lobate margins, with single-celled, transparent, elliptical or oval conidia in chains, regardless temperature or light presence”
e1“Monilinia fructigena is causal agent of brown rot of quince”
Claim · model-written summary
Sclerotia were observed in 14-day-old cultures.
- Taxon
- Monilinia fructigenaMonilinia fructigenae1
- Outcome
- Sclerotiae0
- Qualifier
- 14 days old culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sclerotia are observed in 14 days old cultures”
e1“Monilinia fructigena is causal agent of brown rot of quince”
Claim · model-written summary
Most isolates grew fastest at 27?C in darkness.
- Taxon
- Monilinia fructigenaMonilinia fructigenae1
- Outcome
- growth ratee0
- Direction
- higheste0
- Value
- 27?Ce0
- Qualifier
- in darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest growth rate of most of the isolates is at 27?C and in dark”
e1“Monilinia fructigena is causal agent of brown rot of quince”
Claim · model-written summary
Monilinia fructigena was identified as the causal agent of quince brown rot.
- Taxon
- quinceCydonia oblongae0
- Interactor
- Monilinia fructigenaMonilinia fructigenae0pathogen
- Outcome
- brown rote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Monilinia fructigena is causal agent of brown rot of quince”
Claim · model-written summary
Multiplex PCR amplified the expected 402 bp fragment, confirming the isolates as M. fructigena.
- Taxon
- M. fructigenaMonilinia fructigenae0
- Outcome
- expected fragmente0
- Value
- 402 bpe0
- Qualifier
- Multiplex PCR reactione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expected fragment 402 bp in size was amplified, which confirmed that the studied isolates belonged to the species M. fructigena”
e1“Using specific primers (MO368-5, MO368-8R, MO368-10R, Laxa-R2) for detection of Monilinia species in Multiplex PCR reaction”