Abstract
A highly efficient regeneration system is a prerequisite step for successful genetic transformation of watermelon cultivars (Citrullus lanatus L.). The objective of this study was to establish efficient in vitro plant regeneration for three watermelon cultivars. To achieve optimal conditions for adventitious shoot induction, the 5-day-old explants (cotyledon base portion, apical portion and hypocotyl) of three cultivars were placed on MB5 media supplemented with different concentrations and combinations of growth regulators (1.0 to 10.0 mg L-1 6-benzyladenine (BA) and 0 to 1.0 mg L-1 indole acetic acid (IAA)); the explants from seedling of different development stages (0 to 10 d) were cultured on MB5 medium containing 2.0 mg L-1 BA and 0.2 mg L-1 IAA for investigating the effect of age on adventitious shoots initiation; besides, 5-day-old seedlings were grown on optimal regeneration medium supplemented with different concentrations of kanamycin for screening the lowest lethal concentration for adventitious shoots. The results show that the basal region of cotyledon showed higher frequency of shoot formation (79.17-83.33%) than the apical region (5.23-8.25%); high percentage of shoots regeneration was induced from 5-day-old cotyledons base portion cultured on MB5 containing 1 or 2 mg L-1 BA; the 100 mg L-1 kanamycin proved to be the optimal concentration for screening the transformants. Our results provide an efficient stable regeneration system for genetic transformation of watermelon. Key words: Watermelon (Citrullus lanatus), cotyledon, growth regulator, kanamycin, regeneration.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
Cotyledon basal regions formed shoots more frequently than apical regions.
- Taxon
- watermelon
Citrullus lanatust0 - Organ / stage
- basal region of cotyledone0
- Treatment
- basal region of cotyledone0
- Outcome
- frequency of shoot formatione0
- Direction
- highere0
- Value
- 79.17-83.33%e0
- Qualifier
- 5.23-8.25%e0
- Comparator
- apical regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the basal region of cotyledon showed higher frequency of shoot formation (79.17-83.33%) than the apical region (5.23-8.25%)”
t0“watermelon”
Claim · model-written summary
Five-day-old cotyledon bases on MB5 with 1 or 2 mg L-1 BA had high shoot regeneration.
- Taxon
- watermelonCitrullus lanatust0
- Organ / stage
- 5-day-old cotyledons base portione0
- Treatment
- MB5 containing 1 or 2 mg L-1 BAe0
- Outcome
- shoots regeneratione0
- Direction
- highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high percentage of shoots regeneration was induced from 5-day-old cotyledons base portion cultured on MB5 containing 1 or 2 mg L-1 BA”
t0“watermelon”
Claim · model-written summary
Kanamycin at 100 mg L-1 was optimal for transformant screening.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- 100 mg L-1 kanamycine0
- Outcome
- optimal concentration for screening the transformantse0
- Value
- 100 mg L-1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 100 mg L-1 kanamycin proved to be the optimal concentration for screening the transformants.”
t0“watermelon”
Claim · model-written summary
The results provide a stable watermelon regeneration system for genetic transformation.
- Taxon
- watermelonCitrullus lanatuse0
- Outcome
- efficient stable regeneration system for genetic transformation of watermelone0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results provide an efficient stable regeneration system for genetic transformation of watermelon.”
The same questions in other papers
N-benzyladenine · growth · Citrullus lanatus (watermelon)Positive and negative results
15 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.