Abstract
Constitutive antisense inhibition of the cytosolic isoform of phosphoglucomutase in the potato (Solanum tuberosum L.) results in restriction of photosynthesis, growth inhibition and modified tuber morphology, and a severe restriction of tuber starch synthesis. Here we describe the consequences of the tuber-specific expression of an Escherichia coli phosphoglucomutase in the cytosol. Analysis of [14C]glucose metabolism by tuber discs isolated from wild type and transformants revealed that the rates of sucrose and starch synthesis were unaltered but that the rate of glycolysis was depressed in the transgenics. The transformant tubers also contained dramatically reduced amino acid content and significantly higher levels of ADP, but were characterized by elevated levels of Krebs cycle intermediates and an unaltered rate of respiration. In addition to these metabolic consequences of the overexpression of the E. coli enzyme, we observed morphological changes in tubers, with the transformants having a smaller number of larger tubers which exhibited delayed rates of sprouting with respect to the wild type. These results are discussed with respect to current models of the regulation of central plant metabolism and tuber dormancy.
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Claims 8
Claim · model-written summary
Sucrose and starch synthesis rates were unchanged in transformant tubers.
- Taxon
- Potato
Solanum tuberosumt0 - Treatment
- transgenicse0
- Outcome
- rates of sucrose and starch synthesise0
- Direction
- unalterede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rates of sucrose and starch synthesis were unaltered but that the rate of glycolysis was depressed in the transgenics”
t0“Potato (Solanum tuberosum L.)”
Claim · model-written summary
Glycolysis rate was depressed in transgenic tubers.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- transgenicse0
- Outcome
- rate of glycolysise0
- Direction
- depressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rate of glycolysis was depressed in the transgenics”
t0“Potato (Solanum tuberosum L.)”
Claim · model-written summary
Transformant tuber amino acid content was dramatically reduced.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- transformant tuberse0
- Outcome
- amino acid contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformant tubers also contained dramatically reduced amino acid content”
t0“Potato (Solanum tuberosum L.)”
Claim · model-written summary
Transformant tuber ADP levels were significantly higher.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- transformant tuberse0
- Outcome
- levels of ADPe0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformant tubers also contained dramatically reduced amino acid content and significantly higher levels of ADP”
t0“Potato (Solanum tuberosum L.)”
Claim · model-written summary
Transformant tubers had elevated Krebs cycle intermediates.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- transformant tuberse1
- Outcome
- levels of Krebs cycle intermediatese0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated levels of Krebs cycle intermediates”
t0“Potato (Solanum tuberosum L.)”
e1“transformant tubers also contained dramatically reduced amino acid content”
Claim · model-written summary
Transformant tuber respiration rate was unchanged.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- rate of respiratione0
- Direction
- unalterede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an unaltered rate of respiration”
t0“Potato (Solanum tuberosum L.)”
Claim · model-written summary
Transformants had fewer, larger tubers.
- Taxon
- PotatoSolanum tuberosumt0
- Material
- transformantse0
- Outcome
- smaller number of larger tuberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformants having a smaller number of larger tubers”
t0“Potato (Solanum tuberosum L.)”
Claim · model-written summary
Transformant tubers sprouted later than wild type.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- transformantse0
- Outcome
- rates of sproutinge0
- Direction
- delayede0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformants having a smaller number of larger tubers which exhibited delayed rates of sprouting with respect to the wild type”
t0“Potato (Solanum tuberosum L.)”
The same questions in other papers
transgenic · quality: sugar starch · Solanum tuberosum (potato)Positive and negative results
11 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.