Abstract
The objective in this work was to improve the existing methodology for the detection of Sclerotinia sclerotiorum in dry bean and soybean seeds through the use of water restrictors in place of 2,4-dichlorophenoxyacetic acid, in PDA substrate containing bromophenol blue and antibiotics (Neon-S). Firstly, the effects of the water restrictors mannitol, sodium chloride, and polyethyleneglycol (PEG6000) at different osmotic potentials were evaluated on the mycelial growth of S. sclerotiorum isolates and on the radicle protrusion of seeds of the species studied. The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested. In the second stage of this work, alternating light and continuous darkness were tested in the detection of fungus in the seeds of both species by the modified Neon method. The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory.
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Claims 5
Claim · model-written summary
PEG up to -0.5 MPa did not affect Sclerotinia mycelial growth.
- Taxon
- dry bean and soybean seedst0
- Treatment
- PEGe0
- Interactor
- Sclerotinia sclerotiorum
Sclerotinia sclerotiorumt0pathogen - Outcome
- mycelial growth of the isolatese0
- Direction
- did not affecte0
- Value
- -0.5 MPae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested”
t0“Sclerotinia sclerotiorum in dry bean and soybean seeds”
Claim · model-written summary
Mannitol up to -1.0 MPa did not affect isolate mycelial growth.
- Taxon
- dry bean and soybean seedst0
- Treatment
- mannitole0
- Outcome
- mycelial growth of the isolatese0
- Direction
- did not affecte0
- Value
- -1.0 MPae0
Not reportedComparator
Verbatim
e0“up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested”
t0“dry bean and soybean seeds”
Claim · model-written summary
Sodium chloride up to -1.0 MPa did not affect isolate mycelial growth.
- Taxon
- dry bean and soybean seedst0
- Treatment
- sodium chloridee0
- Outcome
- mycelial growth of the isolatese0
- Direction
- did not affecte0
- Value
- -1.0 MPae0
Not reportedComparator
Verbatim
e0“-1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested”
t0“dry bean and soybean seeds”
Claim · model-written summary
The tested water restrictors inhibited seed radicle protrusion.
- Taxon
- dry bean and soybean seedst0
- Treatment
- water restrictorse0
- Outcome
- radicle protrusione0
- Direction
- inhibite0
Not reportedComparator
Verbatim
e0“The use of water restrictors with osmotic potentials of up to -0.5 MPa for PEG and -1.0 MPa for mannitol and sodium chloride did not affect the mycelial growth of the isolates tested, and proved to be satisfactory to inhibit the radicle protrusion of the seeds tested”
t0“dry bean and soybean seeds”
Claim · model-written summary
The modified Neon method using mannitol in darkness was effective and reliable for routine pathogen detection.
Verbatim
e0“The methodology composed by solid medium PDA, bromophenol blue (100 ppm), chloramphenicol (50 ppm) and water restrictor mannitol (-1.0 MPa) at a temperature of 20 °C with incubation in darkness, proved to be effective and reliable in the detection of that pathogen in routine analysis laboratory”
t0“dry bean and soybean seeds”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.