Abstract
Summary We initiated a proteomics‐based approach to identify root proteins affected by salinity in pea (Pisum sativum cv. Cutlass). Salinity stress was imposed either on 2‐wk old pea plants by watering with salt water over 6 wk or by germinating and growing pea seeds for 7 days in Petri dishes. Concentrations of NaCl above 75 mM had significant negative effects on growth and development of peas in both systems. Salinity‐induced root proteome‐level changes in pea were investigated by 2‐D electrophoresis of proteins from control, 75 and 150 mM NaCl‐treated plants and seedlings. The majority of the protein spots visualised showed reproducible abundance in root protein extracts from whole plants and seedlings. Of these proteins, 35 spots that exhibited significant changes in abundance due to NaCl treatment were selected for identification using ESI‐Q‐TOF MS/MS. The identities of these proteins, which include pathogenesis‐related (PR) 10 proteins, antioxidant enzymes such as superoxide dismutase (SOD) as well as nucleoside diphosphate kinase (NDPK) are presented, and the roles of some of them in mediating responses of pea to salinity are discussed. This is the first report of salinity‐induced changes in the root proteome of pea that suggests a potential role for PR10 proteins in salinity stress responses. Our findings also suggest the possible existence of a novel signal transduction pathway involving SOD, H 2 O 2 , NDPK and PR10 proteins with a potentially crucial role in abiotic stress responses.
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Claims 6
Claim · model-written summary
NaCl above 75 mM significantly impaired pea growth and development in both growth systems.
- Taxon
- peas
Lathyrus oleraceuse0 - Treatment
- NaCl above 75 mMe0
- Outcome
- growth and developmente0
- Direction
- negative effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concentrations of NaCl above 75 mM had significant negative effects on growth and development of peas in both systems.”
Claim · model-written summary
Most visualized root protein spots had reproducible abundance in whole plants and seedlings.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Organ / stage
- roote0
- Outcome
- reproducible abundance in root protein extractse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The majority of the protein spots visualised showed reproducible abundance in root protein extracts from whole plants and seedlings.”
t0“Pisum sativum”
Claim · model-written summary
Thirty-five root protein spots changed significantly in abundance with NaCl treatment.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- NaCl treatmente0
- Outcome
- changes in abundancee0
- Value
- 35 spotse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“35 spots that exhibited significant changes in abundance due to NaCl treatment”
t0“Pisum sativum”
Claim · model-written summary
The salinity-responsive proteins included PR10 proteins, superoxide dismutase and nucleoside diphosphate kinase.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- pathogenesis‐related (PR) 10 proteins, antioxidant enzymes such as superoxide dismutase (SOD) as well as nucleoside diphosphate kinase (NDPK)e0
- Outcome
- identities of these proteinse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The identities of these proteins, which include pathogenesis‐related (PR) 10 proteins, antioxidant enzymes such as superoxide dismutase (SOD) as well as nucleoside diphosphate kinase (NDPK) are presented”
t0“Pisum sativum”
Claim · model-written summary
The authors suggest that PR10 proteins may contribute to pea salinity responses.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PR10 proteinse0
- Outcome
- salinity stress responsese0
- Qualifier
- potential rolee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggests a potential role for PR10 proteins in salinity stress responses”
t0“Pisum sativum”
Claim · model-written summary
The authors suggest a possible abiotic-stress signaling pathway involving SOD, H 2 O 2 , NDPK and PR10 proteins.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Outcome
- abiotic stress responsese0
- Qualifier
- novel signal transduction pathway involving SOD, H 2 O 2 , NDPK and PR10 proteinse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“possible existence of a novel signal transduction pathway involving SOD, H 2 O 2 , NDPK and PR10 proteins with a potentially crucial role in abiotic stress responses”
t0“Pisum sativum”
The same questions in other papers
sodium chloride · growth · Lathyrus oleraceus (pea)Results differ
23 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
- positive
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