Abstract
Bacteria of the genus Azospirillum increase the grain yield of several grass crops. In this work the effect of inoculating maize plants with genetically engineered Azospirillum brasilense for trehalose biosynthesis was determined. Transformed bacteria with a plasmid harboring a trehalose biosynthesis gene-fusion from Saccharomyces cerevisiae were able to grow up to 0.5 M NaCl and to accumulate trehalose, whereas wild-type A. brasilense did not tolerate osmotic stress or accumulate significant levels of the disaccharide. Moreover, 85% of maize plants inoculated with transformed A. brasilense survived drought stress, in contrast with only 55% of plants inoculated with the wild-type strain. A 73% increase in biomass of maize plants inoculated with transformed A. brasilense compared with inoculation with the wild-type strain was found. In addition, there was a significant increase of leaf and root length in maize plants inoculated with transformed A. brasilense. Therefore, inoculation of maize plants with A. brasilense containing higher levels of trehalose confers drought tolerance and a significant increase in leaf and root biomass. This work opens the possibility that A. brasilense modified with a chimeric trehalose biosynthetic gene from yeast could increase the biomass, grain yield and stress tolerance in other relevant crops.
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Claims 5
Claim · model-written summary
Transformed A. brasilense tolerated up to 0.5 M NaCl, unlike wild type.
- Interactor
- A. brasilense
Azospirillum brasilensee0symbiont - Outcome
- grow up to 0.5 M NaCle0
- Value
- 0.5 M NaCle0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformed bacteria with a plasmid harboring a trehalose biosynthesis gene-fusion from Saccharomyces cerevisiae were able to grow up to 0.5 M NaCl and to accumulate trehalose, whereas wild-type A. brasilense did not tolerate osmotic stress or accumulate significant levels of the disaccharide”
t0“maize plants”
Claim · model-written summary
Transformed A. brasilense accumulated trehalose, unlike wild type.
- Interactor
- A. brasilenseAzospirillum brasilensee0symbiont
- Outcome
- accumulate trehalosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformed bacteria with a plasmid harboring a trehalose biosynthesis gene-fusion from Saccharomyces cerevisiae were able to grow up to 0.5 M NaCl and to accumulate trehalose, whereas wild-type A. brasilense did not tolerate osmotic stress or accumulate significant levels of the disaccharide”
t0“maize plants”
Claim · model-written summary
Maize survival under drought was 85% with transformed inoculant versus 55% with wild type.
- Treatment
- inoculated with transformed A. brasilensee0
- Interactor
- A. brasilenseAzospirillum brasilensee0symbiont
- Outcome
- survived drought stresse0
- Direction
- in contrast with onlye0
- Value
- 85%e0
- Qualifier
- 55%e0
- Comparator
- wild-type straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“85% of maize plants inoculated with transformed A. brasilense survived drought stress, in contrast with only 55% of plants inoculated with the wild-type strain”
Claim · model-written summary
Maize biomass increased 73% with transformed inoculant versus wild type.
- Treatment
- inoculated with transformed A. brasilensee0
- Interactor
- A. brasilenseAzospirillum brasilensee0symbiont
- Outcome
- biomasse0
- Direction
- increasee0
- Value
- 73%e0
- Comparator
- wild-type straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 73% increase in biomass of maize plants inoculated with transformed A. brasilense compared with inoculation with the wild-type strain was found”
Claim · model-written summary
Leaf and root length increased significantly in maize with transformed inoculant.
- Treatment
- inoculated with transformed A. brasilensee0
- Interactor
- A. brasilenseAzospirillum brasilensee0symbiont
- Outcome
- leaf and root lengthe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was a significant increase of leaf and root length in maize plants inoculated with transformed A. brasilense”
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