Abstract
Late embryogenesis abundant (LEA) proteins are involved in tolerance to drought, cold and high salinity in many different organisms. In this report, a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa) using the Rapid Amplification of cDNA Ends (RACE) method. OsLEA3-2 was found to be only expressed in the embryo and can be induced by abiotic stresses. The coding protein localizes to the nucleus and overexpression of OsLEA3-2 in yeast improved growth performance compared with control under salt- and osmotic-stress conditions. OsLEA3-2 was also inserted into pHB vector and overexpressed in Arabidopsis and rice. The transgenic Arabidopsis seedlings showed better growth on MS media supplemented with 150 mM mannitol or 100 mM NaCl as compared with wild type plants. The transgenic rice also showed significantly stronger growth performance than control under salinity or osmotic stress conditions and were able to recover after 20 days of drought stress. In vitro analysis showed that OsLEA3-2 was able to protect LDH from aggregation on freezing and inactivation on desiccation. These results indicated that OsLEA3-2 plays an important role in tolerance to abiotic stresses.
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Claims 12
Claim · model-written summary
OsLEA3-2 is expressed only in the embryo of rice.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- embryoe1
- Gene
- OsLEA3-2e0
- Outcome
- expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e1“OsLEA3-2 was found to be only expressed in the embryo and can be induced by abiotic stresses”
Claim · model-written summary
OsLEA3-2 expression can be induced by abiotic stresses.
- Taxon
- riceOryza sativae0
- Treatment
- abiotic stressese1
- Outcome
- expressede1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e1“OsLEA3-2 was found to be only expressed in the embryo and can be induced by abiotic stresses”
Claim · model-written summary
The OsLEA3-2 protein localizes to the nucleus.
- Taxon
- riceOryza sativae0
- Gene
- OsLEA3-2e0
- Outcome
- localizes to the nucleuse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e2“The coding protein localizes to the nucleus”
Claim · model-written summary
Overexpression of OsLEA3-2 in yeast improved growth compared with control under salt and osmotic stress.
- Taxon
- riceOryza sativae0
- Treatment
- overexpression of OsLEA3-2 in yeaste1
- Gene
- OsLEA3-2e0
- Outcome
- growth performancee1
- Direction
- improvede1
- Qualifier
- salt- and osmotic-stress conditionse1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e1“overexpression of OsLEA3-2 in yeast improved growth performance compared with control under salt- and osmotic-stress conditions”
Claim · model-written summary
Transgenic Arabidopsis expressing OsLEA3-2 grew better than wild type on medium with 150 mM mannitol.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- transgenice1
- Treatment
- 150 mM mannitole1
- Gene
- OsLEA3-2e0
- Outcome
- growthe1
- Direction
- bettere1
- Comparator
- wild type plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e1“The transgenic Arabidopsis seedlings showed better growth on MS media supplemented with 150 mM mannitol or 100 mM NaCl as compared with wild type plants”
Claim · model-written summary
Transgenic Arabidopsis expressing OsLEA3-2 grew better than wild type on medium with 100 mM NaCl.
- Taxon
- ArabidopsisArabidopsise1
- Material
- transgenice1
- Treatment
- 100 mM NaCle1
- Gene
- OsLEA3-2e0
- Outcome
- growthe1
- Direction
- bettere1
- Comparator
- wild type plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e1“The transgenic Arabidopsis seedlings showed better growth on MS media supplemented with 150 mM mannitol or 100 mM NaCl as compared with wild type plants”
Claim · model-written summary
Transgenic rice overexpressing OsLEA3-2 grew significantly stronger than control under salinity.
- Taxon
- riceOryza sativae2
- Material
- transgenic ricee2
- Treatment
- salinitye2
- Outcome
- growth performancee2
- Direction
- strongere2
- Comparator
- controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The transgenic rice also showed significantly stronger growth performance than control under salinity or osmotic stress conditions”
Claim · model-written summary
Transgenic rice overexpressing OsLEA3-2 grew significantly stronger than control under osmotic stress.
- Taxon
- riceOryza sativae2
- Material
- transgenic ricee2
- Treatment
- osmotic stresse2
- Outcome
- growth performancee2
- Direction
- strongere2
- Comparator
- controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The transgenic rice also showed significantly stronger growth performance than control under salinity or osmotic stress conditions”
Claim · model-written summary
Transgenic rice overexpressing OsLEA3-2 was able to recover after 20 days of drought stress.
- Taxon
- riceOryza sativae0
- Treatment
- drought stresse3
- Outcome
- able to recovere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e3“were able to recover after 20 days of drought stress”
Claim · model-written summary
In vitro, OsLEA3-2 protected LDH from aggregation on freezing.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e1“In vitro analysis showed that OsLEA3-2 was able to protect LDH from aggregation on freezing and inactivation on desiccation”
Claim · model-written summary
In vitro, OsLEA3-2 protected LDH from inactivation on desiccation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e1“In vitro analysis showed that OsLEA3-2 was able to protect LDH from aggregation on freezing and inactivation on desiccation”
Claim · model-written summary
OsLEA3-2 plays an important role in tolerance to abiotic stresses.
- Taxon
- riceOryza sativae0
- Gene
- OsLEA3-2e4
- Outcome
- tolerance to abiotic stressese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein producing full-length gene OsLEA3-2 was identified in rice (Oryza sativa)”
e4“These results indicated that OsLEA3-2 plays an important role in tolerance to abiotic stresses”
The same questions in other papers
cold · soil: physics · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.