Abstract
The effects of light and glucose in the nutrient medium on the nucleic acid metabolism of excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons were determined. The rates of synthesis as affected by light and glucose were determined by brief exposures to C(14)-labeled orotic acid. The nucleic acids were fractionated by homogenizing in Tris-HCl buffer and centrifuging to obtain soluble and microsomal RNA (20,000 x g supernatant) and a particulate nucleic acid fraction (20,000 x g precipitate) or by extracting in phenol followed by 10% NaCl extraction at 100 degrees . The phenol extract was analyzed by density gradient centrifugation. Light and glucose caused parallel changes in nucleic acid levels of the various fractions, in orotic acid-6-C(14) absorption and in rates of synthesis of nucleic acids. Light and glucose appear to enhance binding of the ribosome nucleic acid so that it becomes less extractable in Tris-HCl buffer or phenol. The bound nucleic acids were labeled at a slightly higher rate than the total nucleic acids extracted by Tris-HCl or phenol. However, light treatment for 48 hours promoted a very high labeling rate in the soluble, low molecular weight nucleic acid as shown by density gradient centrifugation of the phenol extractable fraction.It was concluded that a part of the nucleic acid changes were brought about by light acting through the photosynthetic production of carbohydrate. This conclusion was strengthened by the observation that herbicide inhibitors of photosynthesis and limited atmospheric CO(2) concentrations partially inhibited the nucleic acid changes. However, glucose did not cause changes in nucleic acid levels as large as those caused by light and changes were observed to occur in light even though the endogenous sugar levels were maintained at a low level by the inhibition of photosynthesis with herbicides. The data indicated that light may produce changes in nucleic acid levels by other mechanisms additional to those regulating the sugar level in the tissue.
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Claims 13
Claim · model-written summary
Light caused parallel changes in nucleic acid levels of the various fractions
- Taxon
- Gossypium hirsutum
Gossypium hirsutume0 - Organ / stage
- cotyledonse0
- Treatment
- Lighte1
- Outcome
- nucleic acid levelse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e1“Light and glucose caused parallel changes in nucleic acid levels of the various fractions”
Claim · model-written summary
Glucose caused parallel changes in nucleic acid levels of the various fractions
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Organ / stage
- cotyledonse0
- Treatment
- glucosee1
- Outcome
- nucleic acid levelse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e1“Light and glucose caused parallel changes in nucleic acid levels of the various fractions”
Claim · model-written summary
Glucose caused smaller changes in nucleic acid levels than light
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Treatment
- glucosee2
- Outcome
- nucleic acid levelse2
- Comparator
- lighte2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e2“glucose did not cause changes in nucleic acid levels as large as those caused by light”
Claim · model-written summary
Bound nucleic acids were labeled at a slightly higher rate than total nucleic acids extracted by Tris-HCl or phenol
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Outcome
- labelede3
- Direction
- highere3
- Qualifier
- slightlye3
- Comparator
- total nucleic acids extracted by Tris-HCl or phenole3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e3“The bound nucleic acids were labeled at a slightly higher rate than the total nucleic acids extracted by Tris-HCl or phenol”
Claim · model-written summary
Light treatment for 48 hours promoted a very high labeling rate in the soluble, low molecular weight nucleic acid
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Treatment
- light treatment for 48 hourse4
- Outcome
- labeling ratee4
- Direction
- promotede4
- Value
- very highe4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e4“light treatment for 48 hours promoted a very high labeling rate in the soluble, low molecular weight nucleic acid”
Claim · model-written summary
Herbicide inhibitors of photosynthesis partially inhibited the nucleic acid changes caused by light
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Treatment
- herbicide inhibitors of photosynthesise5
- Outcome
- nucleic acid changese5
- Direction
- partially inhibitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e5“herbicide inhibitors of photosynthesis and limited atmospheric CO(2) concentrations partially inhibited the nucleic acid changes”
Claim · model-written summary
Limited atmospheric CO2 concentrations partially inhibited the nucleic acid changes
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Treatment
- limited atmospheric CO(2) concentrationse5
- Outcome
- nucleic acid changese5
- Direction
- partially inhibitede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e5“herbicide inhibitors of photosynthesis and limited atmospheric CO(2) concentrations partially inhibited the nucleic acid changes”
Claim · model-written summary
Nucleic acid changes occurred in light even when endogenous sugar levels were kept low by herbicide inhibition of photosynthesis
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Treatment
- inhibition of photosynthesis with herbicidese6
- Outcome
- changese6
- Qualifier
- endogenous sugar levels were maintained at a low levele6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e6“changes were observed to occur in light even though the endogenous sugar levels were maintained at a low level by the inhibition of photosynthesis with herbicides”
Claim · model-written summary
Part of the nucleic acid changes caused by light is brought about by light acting through photosynthetic carbohydrate production
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Outcome
- nucleic acid changese7
- Qualifier
- acting through the photosynthetic production of carbohydratee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e7“a part of the nucleic acid changes were brought about by light acting through the photosynthetic production of carbohydrate”
Claim · model-written summary
Light may change nucleic acid levels by mechanisms other than those regulating tissue sugar level
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Outcome
- nucleic acid levelse6
- Qualifier
- by other mechanisms additional to those regulating the sugar level in the tissuee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e6“light may produce changes in nucleic acid levels by other mechanisms additional to those regulating the sugar level in the tissue”
Claim · model-written summary
Light and glucose may enhance binding of ribosome nucleic acid so that it is less extractable in Tris-HCl buffer or phenol
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Outcome
- less extractablee6
- Qualifier
- enhance binding of the ribosome nucleic acide6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e6“Light and glucose appear to enhance binding of the ribosome nucleic acid so that it becomes less extractable in Tris-HCl buffer or phenol”
Claim · model-written summary
Light caused parallel changes in orotic acid-6-C(14) absorption and rates of nucleic acid synthesis
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Treatment
- Lighte7
- Outcome
- orotic acid-6-C(14) absorption and in rates of synthesis of nucleic acidse7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e7“Light and glucose caused parallel changes in nucleic acid levels of the various fractions, in orotic acid-6-C(14) absorption and in rates of synthesis of nucleic acids”
Claim · model-written summary
Glucose caused parallel changes in orotic acid-6-C(14) absorption and rates of nucleic acid synthesis
- Taxon
- Gossypium hirsutumGossypium hirsutume0
- Treatment
- glucosee7
- Outcome
- orotic acid-6-C(14) absorption and in rates of synthesis of nucleic acidse7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excised 8-day cotton (Gossypium hirsutum var. Acala 44) cotyledons”
e7“Light and glucose caused parallel changes in nucleic acid levels of the various fractions, in orotic acid-6-C(14) absorption and in rates of synthesis of nucleic acids”
The same questions in other papers
co2 · molecular: metabolites · Gossypium hirsutum (cotton)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
herbicide · molecular: metabolites · Gossypium hirsutum (cotton)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.