Abstract
Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max L. Merr. Var Hobbit) leaves compared with normal seed-pod-bearing plants. The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activity of 1353 units/mg protein using p-nitrophenylphosphate as the substrate. Isoelectric focusing demonstrated that the purified protein co-migrated with a majority of the activity that increased in leaves following seed-pod removal. Immunoblot analysis demonstrated that at least part of the increased activity was due to an increased abundance of the phosphatase protein. In situ enzyme activity staining localized most of the total phosphatase activity to vascular tissues, the leaf paraveinal mesophyll cell layer, and the lower epidermis. This distribution and the response to seed-pod removal paralleled previous results for soybean vegetative storage protein (VSP) [alpha] and [beta]. However, in a native polyacrylamide gel the VSP detected by immunological staining of electrophoretically transferred protein did not migrate with the majority of the phosphatase activity. Fractionation of crude leaf protein on concanavalin A-Sepharose yielded a fraction containing 97% of the total VSP but only 0.1% of the total acid phosphatase activity.
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Claims 11
Claim · model-written summary
Fruit removal increased soybean leaf acid phosphatase activity tenfold.
- Taxon
- soybean
Glycine maxe0 - Material
- Hobbite0
- Organ / stage
- leavese0
- Treatment
- Fruit removal for 5 weeks after floweringe0
- Gene
- acid phosphatasee0
- Outcome
- acid phosphatase activitye0
- Direction
- increasede0
- Value
- 10-folde0
- Comparator
- normal seed-pod-bearing plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fruit removal for 5 weeks after flowering increased acid phosphatase activity 10-fold in soybean (Glycine max L. Merr. Var Hobbit) leaves compared with normal seed-pod-bearing plants”
Claim · model-written summary
The major leaf acid phosphatase was purified over 2700-fold.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- leavese0
- Gene
- acid phosphatasee0
- Outcome
- major acid phosphatase activitye0
- Value
- 2700-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major acid phosphatase activity in leaves was purified over 2700-fold, yielding a single polypeptide of 51 kD with a specific activity of 1353 units/mg protein using p-nitrophenylphosphate as the substrate”
t0“Soybean”
Claim · model-written summary
Purification yielded a single 51 kD polypeptide.
- Taxon
- SoybeanGlycine maxt0
- Gene
- acid phosphataset1
- Outcome
- single polypeptidee0
- Value
- 51 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“yielding a single polypeptide of 51 kD with a specific activity of 1353 units/mg protein”
t0“Soybean”
t1“Acid Phosphatase”
Claim · model-written summary
The purified enzyme had a specific activity of 1353 units/mg protein.
- Taxon
- SoybeanGlycine maxt0
- Gene
- Acid Phosphataset1
- Outcome
- specific activitye0
- Value
- 1353 units/mg proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a specific activity of 1353 units/mg protein using p-nitrophenylphosphate as the substrate”
t0“Soybean”
t1“Acid Phosphatase”
Claim · model-written summary
The purified protein co-migrated with most phosphatase activity increased after pod removal.
- Taxon
- SoybeanGlycine maxt0
- Gene
- Acid Phosphataset1
- Outcome
- co-migrated with a majority of the activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the purified protein co-migrated with a majority of the activity that increased in leaves following seed-pod removal”
t0“Soybean”
t1“Acid Phosphatase”
Claim · model-written summary
Increased phosphatase protein abundance accounted for at least part of the increased activity.
- Taxon
- SoybeanGlycine maxt0
- Gene
- phosphatase proteine0
- Outcome
- increased activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at least part of the increased activity was due to an increased abundance of the phosphatase protein”
t0“Soybean”
Claim · model-written summary
Most phosphatase activity localized to vascular tissues, paraveinal mesophyll and lower epidermis.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- vascular tissues, the leaf paraveinal mesophyll cell layer, and the lower epidermise0
- Outcome
- total phosphatase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“localized most of the total phosphatase activity to vascular tissues, the leaf paraveinal mesophyll cell layer, and the lower epidermis”
t0“Soybean”
Claim · model-written summary
The phosphatase distribution and response paralleled vegetative storage protein alpha and beta.
- Taxon
- soybeanGlycine maxe0
- Gene
- vegetative storage protein (VSP) [alpha] and [beta]e0
- Outcome
- distribution and the response to seed-pod removale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This distribution and the response to seed-pod removal paralleled previous results for soybean vegetative storage protein (VSP) [alpha] and [beta]”
Claim · model-written summary
Vegetative storage protein did not co-migrate with most phosphatase activity.
- Taxon
- SoybeanGlycine maxt0
- Gene
- VSPe0
- Outcome
- did not migrate with the majority of the phosphatase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the VSP detected by immunological staining of electrophoretically transferred protein did not migrate with the majority of the phosphatase activity”
t0“Soybean”
Claim · model-written summary
The concanavalin A fraction contained 97% of total VSP.
- Taxon
- SoybeanGlycine maxt0
- Gene
- VSPe0
- Outcome
- total VSPe0
- Value
- 97%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“yielded a fraction containing 97% of the total VSP but only 0.1% of the total acid phosphatase activity”
t0“Soybean”
Claim · model-written summary
The concanavalin A fraction contained only 0.1% of total acid phosphatase activity.
- Taxon
- SoybeanGlycine maxt0
- Gene
- acid phosphatasee0
- Outcome
- total acid phosphatase activitye0
- Value
- 0.1%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“yielded a fraction containing 97% of the total VSP but only 0.1% of the total acid phosphatase activity”
t0“Soybean”
The same questions in other papers
stage · soil: biology · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.