Abstract
Ferredoxin is a nuclear-encoded protein that is involved in a variety of electron transfer reactions in both photosynthetic and non-photosynthetic plastids. We show here that the expression of the ferredoxin A gene (FedA) in Arabidopsis thaliana is light-regulated, with its mRNA level increased 4.5-fold by transfer of dark-grown seedlings to white light for 3 h. A portion of this light regulation is mediated by phytochrome through a very low fluence type of response. In addition, it is likely that another photoreceptor(s) is also involved. The FedA promoter confers a light- and tissue-regulated expression pattern when fused to the beta-glucuronidase (GUS) and luciferase reporter genes, indicating that the gene is transcriptionally regulated. No evidence of cis-acting light-regulatory elements within the 5' untranslated leader region of the gene was detected. Nevertheless, elements within this leader are required for full activity since its deletion reduces expression both in the light and dark by 25-fold. This region includes a sequence, ACAAAA, which is also present in the 5' untranslated leader of the other three ferredoxin leaders that have been sequenced and in the leaders of 31 other plant genes or about 8% of all plant genes in the GenBank database. In addition to mediating light regulation, the FedA promoter also directs GUS expression in aerial tissues at 70-fold higher levels than in roots. GUS activity staining in aerial tissues is observed in both photosynthetic and non-photosynthetic cells. These data indicate that the FedA promoter also carries the information for expression of this gene in a highly tissue- and cell-specific manner.
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Claims 10
Claim · model-written summary
White light increased FedA mRNA 4.5-fold after 3 hours in dark-grown Arabidopsis seedlings.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- white light for 3 he0
- Gene
- FedAe1
- Outcome
- mRNA levele0
- Direction
- increasede0
- Value
- 4.5-folde0
- Comparator
- dark-grown seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its mRNA level increased 4.5-fold by transfer of dark-grown seedlings to white light for 3 h”
e1“ferredoxin A gene (FedA) in Arabidopsis thaliana”
Claim · model-written summary
Phytochrome mediated part of FedA light regulation through a very low fluence response.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- phytochromee0
- Outcome
- light regulatione0
- Qualifier
- very low fluence type of responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A portion of this light regulation is mediated by phytochrome through a very low fluence type of response”
e1“FedA) in Arabidopsis thaliana”
Claim · model-written summary
Another photoreceptor likely contributes to FedA light regulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Outcome
- light regulatione1
- Qualifier
- another photoreceptor(s)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it is likely that another photoreceptor(s) is also involved”
e1“light regulation is mediated by phytochrome through a very low fluence type of response”
e2“ferredoxin A gene (FedA) in Arabidopsis thaliana”
t0“Arabidopsis”
Claim · model-written summary
The FedA promoter conferred light- and tissue-regulated reporter expression, supporting transcriptional regulation.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- FedAe0
- Outcome
- light- and tissue-regulated expression patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The FedA promoter confers a light- and tissue-regulated expression pattern when fused to the beta-glucuronidase (GUS) and luciferase reporter genes, indicating that the gene is transcriptionally regulated”
t0“Arabidopsis”
Claim · model-written summary
No cis-acting light-regulatory element was detected in the FedA 5-prime untranslated leader.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- FedAe1
- Outcome
- cis-acting light-regulatory elements within the 5' untranslated leader regione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No evidence of cis-acting light-regulatory elements within the 5' untranslated leader region of the gene was detected”
t0“Arabidopsis ferredoxin A gene”
e1“ferredoxin A gene (FedA) in Arabidopsis thaliana”
Claim · model-written summary
Deleting the FedA leader reduced expression in light and dark by 25-fold.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- deletione0
- Gene
- FedAe1
- Outcome
- expressione0
- Direction
- reducese0
- Value
- 25-folde0
- Qualifier
- both in the light and darke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its deletion reduces expression both in the light and dark by 25-fold”
t0“Arabidopsis ferredoxin A gene”
e1“ferredoxin A gene (FedA) in Arabidopsis thaliana”
Claim · model-written summary
The ACAAAA leader sequence was shared with three other sequenced ferredoxin leaders.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ACAAAAe0
- Outcome
- present in the 5' untranslated leader of the other three ferredoxin leaderse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACAAAA, which is also present in the 5' untranslated leader of the other three ferredoxin leaders that have been sequenced”
t0“Arabidopsis”
Claim · model-written summary
The ACAAAA leader sequence was present in 31 other plant genes, about 8% of plant genes in GenBank.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ACAAAAe1
- Outcome
- in the leaders of 31 other plant genese0
- Value
- about 8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and in the leaders of 31 other plant genes or about 8% of all plant genes in the GenBank database”
t0“Arabidopsis”
e1“a sequence, ACAAAA”
Claim · model-written summary
The FedA promoter directed GUS expression 70-fold higher in aerial tissues than roots.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- aerial tissuese0
- Gene
- FedAe0
- Outcome
- GUS expressione0
- Direction
- highere0
- Value
- 70-folde0
- Comparator
- rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the FedA promoter also directs GUS expression in aerial tissues at 70-fold higher levels than in roots”
t0“Arabidopsis”
Claim · model-written summary
GUS staining occurred in both photosynthetic and non-photosynthetic aerial cells.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- aerial tissuese0
- Outcome
- GUS activity staininge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS activity staining in aerial tissues is observed in both photosynthetic and non-photosynthetic cells”
t0“Arabidopsis”
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See all 197 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.