Abstract
Using a cDNA expression cassette in combination with agroinoculation of potato leaf discs we have investigated the role the protein encoded by ORF0 of Potato leafroll virus (PLRV) and have shown its importance for virus accumulation. Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells. They did not, however, affect translation of ORF1 and ORF2. We therefore conclude that ORF0 of PLRV produces a protein essential for virus accumulation, a hitherto undescribed finding.
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Claims 3
Claim · model-written summary
Two ORF0 mutations prevented expression of the corresponding protein.
- Taxon
- Potato
Solanum tuberosumt0 - Treatment
- Two mutations introduced into ORF0e0
- Gene
- ORF0e0
- Outcome
- expression of the corresponding proteine0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein”
t0“Potato”
Claim · model-written summary
The two ORF0 mutations abolished virus accumulation in plant cells.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- Two mutations introduced into ORF0e0
- Gene
- ORF0e0
- Outcome
- virus accumulation in plant cellse0
- Direction
- completely abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells”
t0“Potato”
Claim · model-written summary
The mutations did not affect translation of ORF1 and ORF2.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- ORF1 and ORF2e0
- Outcome
- translation of ORF1 and ORF2e0
- Direction
- did not, however, affecte0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They did not, however, affect translation of ORF1 and ORF2.”
t0“Potato leafroll virus”
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