Paper · 1972 · Plant and Cell Physiology

Effect of ammonium nutrition on the activity of nitrate reductase in the roots of apple seedlings

G. J. T. Frith

5
claims
10
verbatim quotes
32
citations
2
research areas
—
Not open access

Abstract

Active extracts of nitrate reductase were prepared from the roots of apple seedlings c.v. Granny Smith which were grown in nutrient solution under controlled enviromental conditions. The nutrient solutions contained various ratios of nitrate and ammonium ions but all the treatments contained a total of 112 ppm nitrogen. Maximum nitrate reductase activity in the roots was obtained when plants were supplied with nitrate as the sole source of nitrogen. Roots grown in solution containing only ammonium nitrogen had little or no activity. When plants were supplied with both forms of nitrogen in the nutrient solution, the presence of ammonium ions markedly lowered the activity of nitrate reductase in the roots. Plants supplied with 98 ppm nitrate nitrogen plus 14 ppm ammonium nitrogen had activities only half those of plants grown in nitrate alone. Plants supplied with equal amounts of ammonium and nitrate nitrogen had activities less than one sixth those of plants grown in nitrate alone.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nitrate alone gave maximum apple root nitrate reductase activity.

Taxon
apple seedlingsMalus domesticat0
Treatment
nitrate as the sole source of nitrogene0
Outcome
nitrate reductase activity in the rootse0
Direction
Maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Maximum nitrate reductase activity in the roots was obtained when plants were supplied with nitrate as the sole source of nitrogen”

✓ located verbatim in the source · abstract, characters 322–452 · show in text ↑
t0

“apple seedlings”

✓ located verbatim in the source · title, characters 82–97 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Ammonium alone yielded little or no root nitrate reductase activity.

Taxon
apple seedlingsMalus domesticat0
Treatment
only ammonium nitrogene0
Outcome
activitye0
Direction
little or noe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Roots grown in solution containing only ammonium nitrogen had little or no activity”

✓ located verbatim in the source · abstract, characters 454–537 · show in text ↑
t0

“apple seedlings”

✓ located verbatim in the source · title, characters 82–97 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Ammonium in mixed nitrogen nutrition markedly lowered apple root nitrate reductase activity.

Taxon
apple seedlingsMalus domesticat0
Treatment
ammonium ionse0
Outcome
activity of nitrate reductase in the rootse0
Direction
lowerede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“the presence of ammonium ions markedly lowered the activity of nitrate reductase in the roots”

✓ located verbatim in the source · abstract, characters 619–712 · show in text ↑
t0

“apple seedlings”

✓ located verbatim in the source · title, characters 82–97 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 98 ppm nitrate plus 14 ppm ammonium, activity was half that under nitrate alone.

Taxon
apple seedlingsMalus domesticat0
Treatment
98 ppm nitrate nitrogen plus 14 ppm ammonium nitrogene0
Outcome
activitiese0
Direction
only halfe0
Value
halfe0
Comparator
plants grown in nitrate alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Plants supplied with 98 ppm nitrate nitrogen plus 14 ppm ammonium nitrogen had activities only half those of plants grown in nitrate alone”

✓ located verbatim in the source · abstract, characters 714–852 · show in text ↑
t0

“apple seedlings”

✓ located verbatim in the source · title, characters 82–97 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Equal ammonium and nitrate produced less than one sixth the activity of nitrate alone.

Taxon
apple seedlingsMalus domesticat0
Treatment
equal amounts of ammonium and nitrate nitrogene0
Outcome
activitiese0
Direction
less thane0
Value
one sixthe0
Comparator
plants grown in nitrate alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Plants supplied with equal amounts of ammonium and nitrate nitrogen had activities less than one sixth those of plants grown in nitrate alone”

✓ located verbatim in the source · abstract, characters 854–995 · show in text ↑
t0

“apple seedlings”

✓ located verbatim in the source · title, characters 82–97 · show in text ↑

nitrogen · physiology: enzyme activity · Malus domestica (apple)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.