Abstract
ABSTRACT Fusarium subglutinans has been associated with mango floral and vegetative malformation, although confusion exists regarding the etiology of the disease. A wild-type isolate of F. subglutinans causing mango malformation disease was transformed with the GUS (beta-glucuronidase) reporter and hygromycin resistance genes. Five stable transformants were isolated containing varying copy numbers at different integration sites. Specific GUS activity was quantified for the transformants, whereas no activity was recorded for the wild-type isolate. The transformants and the wild-type isolate were inoculated into healthy mango floral and vegetative buds. Typical symptoms of misshapen shoots with short internodes, stubby leaves, and bunchy, malformed inflorescences were observed 6 to 8 weeks following inoculation. The presence of GUS-stained mycelium of the pathogen viewed microscopically within infected plant organs provided unequivocal evidence that F. subglutinans is indeed a causal agent of mango malformation disease.
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Claims 4
Claim · model-written summary
Five stable transformed isolates had variable gene copy numbers and integration sites.
- Taxon
- mango
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Mangifera indicae1 - Gene
- GUS (beta-glucuronidase) reporter and hygromycin resistance genese2
- Outcome
- Five stable transformantse0
- Value
- Fivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_from_India.jpg?width=500)
Verbatim
e0“Five stable transformants were isolated containing varying copy numbers at different integration sites.”
e1“mango floral and vegetative malformation”
e2“GUS (beta-glucuronidase) reporter and hygromycin resistance genes”
Claim · model-written summary
Transformants had specific GUS activity whereas the wild-type isolate had none.
- Taxon
- mangoMangifera indicae1
- Outcome
- Specific GUS activitye0
- Comparator
- wild-type isolatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Specific GUS activity was quantified for the transformants, whereas no activity was recorded for the wild-type isolate.”
e1“mango floral and vegetative malformation”
Claim · model-written summary
Inoculated mango buds developed characteristic malformation symptoms after 6 to 8 weeks.
- Taxon
- mangoMangifera indicae0
- Treatment
- inoculatede0
- Interactor
- Fusarium subglutinans
Fusarium subglutinanse1pathogen - Outcome
- Typical symptoms of misshapen shoots with short internodes, stubby leaves, and bunchy, malformed inflorescencese0
- Value
- 6 to 8 weekse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transformants and the wild-type isolate were inoculated into healthy mango floral and vegetative buds. Typical symptoms of misshapen shoots with short internodes, stubby leaves, and bunchy, malformed inflorescences were observed 6 to 8 weeks following inoculation.”
e1“Fusarium subglutinans has been associated with mango floral and vegetative malformation”
Claim · model-written summary
GUS-stained pathogen mycelium in infected mango organs supported F. subglutinans as a causal agent.
- Taxon
- mangoMangifera indicae0
- Interactor
- F. subglutinansFusarium subglutinanse0pathogen
- Outcome
- presence of GUS-stained mycelium of the pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of GUS-stained mycelium of the pathogen viewed microscopically within infected plant organs provided unequivocal evidence that F. subglutinans is indeed a causal agent of mango malformation disease.”
The same questions in other papers
inoculation · Fusarium subglutinans · Mangifera indica (mango)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.