Abstract
In the present study, out of 264 phosphate (P) solubilizing Bacillus strains isolated from apple rhizosphere, only twelve isolates were found to be efficient (showed most of the plant growth promoting activity) which were further characterized at molecular level using 16S rDNA partial gene sequencing. Out of 12 isolates, MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) isolate which also showed indole acetic acid production (51.83 μg/ml), siderophore production, ammonia production, antagonistic property (against Rhizoctonia solani and Fusarium oxysporum), hydrolytic enzymes productions (protease, chitinase and cellulase), 1-aminocyclopropane-1-carboxylate (ACC) deaminase production (7.7 μm αKB mg(-1) h(-1)). The in-vitro seed germination assay showed that Bacillus (twelve isolates) inoculated seeds showed more seed germination and seedling vigor rate as compared to uninoculated control treatment. For the genetic diversity studies of efficient 12 strains, the polyphasic approach using 16S-rDNA, Repetitive element sequence (rep) based PCR (ERIC-PCR and BOX-PCR) were used. Based on 16S rDNA partial gene sequencing the isolated Bacillus genus was divide into four groups. First group (five isolates), second group (two isolates), third group (three isolates) and fourth group (two isolates) which showed close genetic relatedness to the B. subtilis, B. pumulis, B. megaterium and B. amyloliquefaciens, respectively. The rep PCR fingerprinting showed variability between and within the species. The large variability was showed by ERIC-PCR whereas some variability was showed by BOX-PCR. The results clearly showed that 16S rRNA gene sequencing is unable to discriminate the isolates at strain level. But rep-PCR fingerprinting is excellent tool to characterize and discriminate the strains at the genomic level.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 15
Claim · model-written summary
Twelve of 264 apple rhizosphere Bacillus isolates were efficient plant growth promoters.
- Taxon
- apple
Malus domesticae0 - Interactor
- Bacilluse0symbiont
- Outcome
- plant growth promoting activitye0
- Value
- twelve isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“out of 264 phosphate (P) solubilizing Bacillus strains isolated from apple rhizosphere, only twelve isolates were found to be efficient (showed most of the plant growth promoting activity)”
Claim · model-written summary
MZPSB 207 was the most efficient phosphate solubilizer, at 864.71 μg/ml.
- Taxon
- appleMalus domesticae1
- Interactor
- MZPSB 207e0symbiont
- Outcome
- P-solubilizinge0
- Value
- 864.71 μg/mle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) isolate”
e1“apple rhizosphere”
Claim · model-written summary
MZPSB 207 produced indole acetic acid at 51.83 μg/ml.
- Taxon
- appleMalus domesticae1
- Interactor
- MZPSB 207e0symbiont
- Outcome
- indole acetic acid productione0
- Value
- 51.83 μg/mle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) isolate which also showed indole acetic acid production (51.83 μg/ml)”
e1“apple rhizosphere”
Claim · model-written summary
MZPSB 207 produced siderophores and ammonia.
- Taxon
- appleMalus domesticae1
- Interactor
- MZPSB 207e0symbiont
- Outcome
- siderophore production, ammonia productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) isolate which also showed indole acetic acid production (51.83 μg/ml), siderophore production, ammonia production”
e1“apple rhizosphere”
Claim · model-written summary
MZPSB 207 showed antagonism against Rhizoctonia solani.
- Taxon
- appleMalus domesticae2
- Interactor
- Rhizoctonia solani
Rhizoctonia solanie0pathogen - Second interactor
- MZPSB 207e1biocontrol_agent
- Outcome
- antagonistic propertye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“antagonistic property (against Rhizoctonia solani and Fusarium oxysporum)”
e1“MZPSB 207 was found to be most efficient”
e2“apple rhizosphere”
Claim · model-written summary
MZPSB 207 showed antagonism against Fusarium oxysporum.
- Taxon
- appleMalus domesticae2
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Second interactor
- MZPSB 207e1biocontrol_agent
- Outcome
- antagonistic propertye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“antagonistic property (against Rhizoctonia solani and Fusarium oxysporum)”
e1“MZPSB 207 was found to be most efficient”
e2“apple rhizosphere”
Claim · model-written summary
MZPSB 207 produced protease, chitinase, and cellulase.
- Taxon
- appleMalus domesticae2
- Interactor
- MZPSB 207e1symbiont
- Outcome
- hydrolytic enzymes productionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydrolytic enzymes productions (protease, chitinase and cellulase)”
e1“MZPSB 207 was found to be most efficient”
e2“apple rhizosphere”
Claim · model-written summary
MZPSB 207 produced ACC deaminase at 7.7 μm αKB mg(-1) h(-1).
- Taxon
- appleMalus domesticae2
- Interactor
- MZPSB 207e1symbiont
- Outcome
- 1-aminocyclopropane-1-carboxylate (ACC) deaminase productione0
- Value
- 7.7 μm αKB mg(-1) h(-1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1-aminocyclopropane-1-carboxylate (ACC) deaminase production (7.7 μm αKB mg(-1) h(-1))”
e1“MZPSB 207 was found to be most efficient”
e2“apple rhizosphere”
Claim · model-written summary
Bacillus inoculation increased seed germination relative to uninoculated control.
- Taxon
- appleMalus domesticae1
- Treatment
- Bacillus (twelve isolates) inoculatede0
- Interactor
- Bacilluse0symbiont
- Outcome
- seed germinatione0
- Direction
- moree0
- Comparator
- uninoculated control treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in-vitro seed germination assay showed that Bacillus (twelve isolates) inoculated seeds showed more seed germination and seedling vigor rate as compared to uninoculated control treatment.”
e1“apple rhizosphere”
Claim · model-written summary
Bacillus inoculation increased seedling vigor relative to uninoculated control.
- Taxon
- appleMalus domesticae1
- Treatment
- Bacillus (twelve isolates) inoculatede0
- Interactor
- Bacilluse0symbiont
- Outcome
- seedling vigor ratee0
- Direction
- moree0
- Comparator
- uninoculated control treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in-vitro seed germination assay showed that Bacillus (twelve isolates) inoculated seeds showed more seed germination and seedling vigor rate as compared to uninoculated control treatment.”
e1“apple rhizosphere”
Claim · model-written summary
16S sequencing separated the Bacillus isolates into four groups related to four named species.
- Taxon
- appleMalus domesticae1
- Interactor
- Bacilluse0symbiont
- Outcome
- four groupse0
- Value
- four groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Based on 16S rDNA partial gene sequencing the isolated Bacillus genus was divide into four groups. First group (five isolates), second group (two isolates), third group (three isolates) and fourth group (two isolates) which showed close genetic relatedness to the B. subtilis, B. pumulis, B. megaterium and B. amyloliquefaciens, respectively.”
e1“apple rhizosphere”
Claim · model-written summary
Rep-PCR fingerprints varied between and within Bacillus species.
- Taxon
- appleMalus domesticae1
- Interactor
- Bacilluse1symbiont
- Outcome
- variability between and within the speciese0
- Comparator
- between and within the speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rep PCR fingerprinting showed variability between and within the species.”
e1“Bacillus strains isolated from apple rhizosphere”
Claim · model-written summary
ERIC-PCR showed large variability, whereas BOX-PCR showed some variability.
- Taxon
- appleMalus domesticae1
- Interactor
- Bacilluse1symbiont
- Outcome
- large variabilitye0
- Comparator
- BOX-PCRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The large variability was showed by ERIC-PCR whereas some variability was showed by BOX-PCR.”
e1“Bacillus strains isolated from apple rhizosphere”
Claim · model-written summary
16S rRNA sequencing could not discriminate isolates at strain level.
- Taxon
- appleMalus domesticae1
- Interactor
- Bacilluse1symbiont
- Outcome
- discriminate the isolates at strain levele0
- Value
- 16S rRNA gene sequencinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“16S rRNA gene sequencing is unable to discriminate the isolates at strain level.”
e1“Bacillus strains isolated from apple rhizosphere”
Claim · model-written summary
Rep-PCR fingerprints characterized and discriminated strains at genomic level.
- Taxon
- appleMalus domesticae1
- Interactor
- Bacilluse1symbiont
- Outcome
- characterize and discriminate the strains at the genomic levele0
- Value
- rep-PCR fingerprintinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rep-PCR fingerprinting is excellent tool to characterize and discriminate the strains at the genomic level.”
e1“Bacillus strains isolated from apple rhizosphere”
The same questions in other papers
inoculation · growth · Malus domestica (apple)Positive and negative results
30 papers in the same direction as this paper · 11 in the opposite direction
- negative
- negative
- negative
38 papers more in this group
See all 42 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.