Abstract
A screen for Arabidopsis mutants that were insensitive to methyl jasmonate (MeJA) in an assay for seedling root growth yielded only alleles of previously isolated mutants jar1 and coi1, with one exception. Mapping of the locus and morphological characterization of the new mutant suggested it might be allelic to axr1, which had not previously been reported to show resistance to MeJA. The F(1) from a cross of the new mutant with axr1-3 did not show complementation, confirming that these are the same genes. The new allele is called axr1-24. In addition to MeJA and indole-3-acetic acid (IAA), axr1-24 had decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine, epi-brassinolide, and abscisic acid. Both axr1-24 and the previously characterized axr1-3 allele were shown to be susceptible to the opportunistic pathogen Pythium irregulare, a trait found in other jasmonate response mutants, including jar1-1. The double mutant jar1-1/axr1-3 was more resistant to inhibition of root growth by MeJA and was more susceptible to P. irregulare infection than either single mutant, suggesting these genes might act in independent response pathways. In contrast, resistance to IAA in the double mutant was not different from axr1-3. Northern-blot analysis showed that IAA induced the jasmonate-responsive lipoxygenase 2, AOS, and AtVSP gene transcripts and induction was strongly impaired in axr1-3. However, transcript induction by MeJA was only minimally affected in axr1-3. This study demonstrates that in addition to auxin signaling, the AXR1 locus is involved in MeJA response, providing a mechanistic link between jasmonate and auxin-signaling pathways.
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Claims 19
Claim · model-written summary
The methyl jasmonate insensitivity screen yielded jar1 and coi1 alleles and one exceptional mutant.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- jar1 and coi1e0
- Outcome
- alleles of previously isolated mutants jar1 and coi1, with one exceptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A screen for Arabidopsis mutants that were insensitive to methyl jasmonate (MeJA) in an assay for seedling root growth yielded only alleles of previously isolated mutants jar1 and coi1, with one exception.”
t0“Arabidopsis”
Claim · model-written summary
The new mutant did not complement axr1-3, establishing allelism.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- axr1-3e0
- Outcome
- did not show complementatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The F(1) from a cross of the new mutant with axr1-3 did not show complementation, confirming that these are the same genes.”
t0“Arabidopsis”
Claim · model-written summary
The new allele was designated axr1-24.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- axr1-24e0
- Outcome
- axr1-24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The new allele is called axr1-24.”
t0“Arabidopsis”
Claim · model-written summary
axr1-24 showed decreased sensitivity to MeJA.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MeJAe0
- Gene
- axr1-24e0
- Outcome
- decreased sensitivitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to MeJA and indole-3-acetic acid (IAA), axr1-24 had decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine, epi-brassinolide, and abscisic acid.”
t0“Arabidopsis”
Claim · model-written summary
axr1-24 showed decreased sensitivity to indole-3-acetic acid (IAA).
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- indole-3-acetic acid (IAA)e0
- Gene
- axr1-24e0
- Outcome
- decreased sensitivitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to MeJA and indole-3-acetic acid (IAA), axr1-24 had decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine, epi-brassinolide, and abscisic acid.”
t0“Arabidopsis”
Claim · model-written summary
axr1-24 showed decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 1-aminocyclopropane-1-carboxylic acide0
- Gene
- axr1-24e0
- Outcome
- decreased sensitivitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to MeJA and indole-3-acetic acid (IAA), axr1-24 had decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine, epi-brassinolide, and abscisic acid.”
t0“Arabidopsis”
Claim · model-written summary
axr1-24 showed decreased sensitivity to 6-benzylamino-purine.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- 6-benzylamino-purinee0
- Gene
- axr1-24e0
- Outcome
- decreased sensitivitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to MeJA and indole-3-acetic acid (IAA), axr1-24 had decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine, epi-brassinolide, and abscisic acid.”
t0“Arabidopsis”
Claim · model-written summary
axr1-24 showed decreased sensitivity to epi-brassinolide.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- epi-brassinolidee0
- Gene
- axr1-24e0
- Outcome
- decreased sensitivitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to MeJA and indole-3-acetic acid (IAA), axr1-24 had decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine, epi-brassinolide, and abscisic acid.”
t0“Arabidopsis”
Claim · model-written summary
axr1-24 showed decreased sensitivity to abscisic acid.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- abscisic acide0
- Gene
- axr1-24e0
- Outcome
- decreased sensitivitye0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition to MeJA and indole-3-acetic acid (IAA), axr1-24 had decreased sensitivity to 1-aminocyclopropane-1-carboxylic acid, 6-benzylamino-purine, epi-brassinolide, and abscisic acid.”
t0“Arabidopsis”
Claim · model-written summary
axr1-24 was susceptible to Pythium irregulare.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- Pythium irregulareGlobisporangium irregularee0pathogen
- Gene
- axr1-24e0
- Outcome
- susceptible to the opportunistic pathogen Pythium irregularee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both axr1-24 and the previously characterized axr1-3 allele were shown to be susceptible to the opportunistic pathogen Pythium irregulare, a trait found in other jasmonate response mutants, including jar1-1.”
t0“Arabidopsis”
Claim · model-written summary
axr1-3 was susceptible to Pythium irregulare.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- Pythium irregulareGlobisporangium irregularee0pathogen
- Gene
- axr1-3e0
- Outcome
- susceptible to the opportunistic pathogen Pythium irregularee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both axr1-24 and the previously characterized axr1-3 allele were shown to be susceptible to the opportunistic pathogen Pythium irregulare, a trait found in other jasmonate response mutants, including jar1-1.”
t0“Arabidopsis”
Claim · model-written summary
The double mutant was more resistant to MeJA inhibition of root growth than either single mutant.
- Taxon
- ArabidopsisArabidopsist0
- Material
- jar1-1/axr1-3e0
- Treatment
- MeJAe0
- Outcome
- inhibition of root growth by MeJAe0
- Direction
- more resistante0
- Comparator
- either single mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The double mutant jar1-1/axr1-3 was more resistant to inhibition of root growth by MeJA and was more susceptible to P. irregulare infection than either single mutant, suggesting these genes might act in independent response pathways.”
t0“Arabidopsis”
Claim · model-written summary
The double mutant was more susceptible to P. irregulare infection than either single mutant.
- Taxon
- ArabidopsisArabidopsist0
- Material
- jar1-1/axr1-3e0
- Interactor
- P. irregulareGlobisporangium irregularee0pathogen
- Outcome
- P. irregulare infectione0
- Direction
- more susceptiblee0
- Comparator
- either single mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The double mutant jar1-1/axr1-3 was more resistant to inhibition of root growth by MeJA and was more susceptible to P. irregulare infection than either single mutant, suggesting these genes might act in independent response pathways.”
t0“Arabidopsis”
Claim · model-written summary
The two genes may act in independent response pathways.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- jar1-1/axr1-3e0
- Outcome
- independent response pathwayse0
- Qualifier
- suggesting these genes might acte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The double mutant jar1-1/axr1-3 was more resistant to inhibition of root growth by MeJA and was more susceptible to P. irregulare infection than either single mutant, suggesting these genes might act in independent response pathways.”
t0“Arabidopsis”
Claim · model-written summary
Double-mutant resistance to IAA did not differ from axr1-3.
- Taxon
- ArabidopsisArabidopsist0
- Material
- double mutante0
- Outcome
- resistance to IAAe0
- Direction
- not differente0
- Comparator
- axr1-3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In contrast, resistance to IAA in the double mutant was not different from axr1-3.”
t0“Arabidopsis”
Claim · model-written summary
IAA induced lipoxygenase 2, AOS and AtVSP transcripts.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- IAAe0
- Gene
- lipoxygenase 2, AOS, and AtVSPe0
- Outcome
- gene transcriptse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Northern-blot analysis showed that IAA induced the jasmonate-responsive lipoxygenase 2, AOS, and AtVSP gene transcripts and induction was strongly impaired in axr1-3.”
t0“Arabidopsis”
Claim · model-written summary
IAA induction of these transcripts was strongly impaired in axr1-3.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- IAAe0
- Gene
- axr1-3e0
- Outcome
- inductione0
- Direction
- strongly impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Northern-blot analysis showed that IAA induced the jasmonate-responsive lipoxygenase 2, AOS, and AtVSP gene transcripts and induction was strongly impaired in axr1-3.”
t0“Arabidopsis”
Claim · model-written summary
MeJA-induced transcript expression was minimally affected in axr1-3.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MeJAe0
- Gene
- axr1-3e0
- Outcome
- transcript inductione0
- Direction
- minimally affectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, transcript induction by MeJA was only minimally affected in axr1-3.”
t0“Arabidopsis”
Claim · model-written summary
AXR1 is involved in MeJA response, linking jasmonate and auxin signaling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AXR1e0
- Outcome
- MeJA responsee0
- Qualifier
- mechanistic link between jasmonate and auxin-signaling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study demonstrates that in addition to auxin signaling, the AXR1 locus is involved in MeJA response, providing a mechanistic link between jasmonate and auxin-signaling pathways.”
t0“Arabidopsis”
The same questions in other papers
indole-3-acetic acid · molecular: expression · ArabidopsisPositive and negative results
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.