Abstract
A forward molecular genetics approach was used to isolate gh3.9-1 and D-fbr7 from populations of Arabidopsis activation-tagged T-DNA mutants in a germination/development-based selection scheme to identify fumonisin B1-resistant (fbr) mutants. The characterization of the functions of affected genes in gh3.9-1 and D-fbr7 is the primary focus of the studies presented in this thesis. The GH3.9 gene, belonging to the Arabidopsis Group II primary auxin-responsive GH3 gene family, was found to be affected by the T-DNA insertion in the gh3.9-1 mutant. Phenotypic characterization studies performed with gh3.9-1 mutant along with GH3.9 RNAi lines and additional T-DNA insertions identified in the same region, revealed that GH3.9 might be involved in root development-related processes, as the mutants displayed a long-root length phenotype. GH3.9 expression analysis revealed that GH3.9 transcript accumulation was repressed in seedlings treated with exogenous IAA treatment. Furthermore, gh3.9-1 T-DNA insertions and RNAi lines showed sensitivity to IAA-mediated root growth inhibition and resistance towards Methyl Jasmonate-mediated root growth inhibition. These results have provided new functions for GH3.9 in roots, where it likely controls auxin activity through its amino acid conjugation activity. D-fbr7 was identified as a novel FB1-resistant ( fbr) mutant that was subsequently also found to be defective in embryo development. Different physiological assays conducted to determine resistance of D-fbr7 to FB1-induced cell death at different stages of plant development showed that at both seedling and mature development stages, D-fbr7 displayed resistance to FB1 and FB1-induced senescence. Expression analysis revealed that CLE19, member of CLAVATA3-LIKE (CLE) gene family is involved in causing the fbr phenotype and small RNAs identified in the intergenic region adjacent to D-fbr7 might be involved in conferring embryo lethality. These small RNAs identified by web-based computational approaches were validated by northern expression analysis. These studies have provided evidence of novel roles for CLE19 and small RNAs in affecting plant development.
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Claims 13
Claim · model-written summary
The GH3.9 gene was affected by the T-DNA insertion in gh3.9-1.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- gh3.9-1e0
- Gene
- GH3.9e0
- Outcome
- affected by the T-DNA insertione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GH3.9 gene, belonging to the Arabidopsis Group II primary auxin-responsive GH3 gene family, was found to be affected by the T-DNA insertion in the gh3.9-1 mutant”
Claim · model-written summary
GH3.9 mutants had a long root phenotype, suggesting GH3.9 involvement in root development.
- Taxon
- ArabidopsisArabidopsist0
- Material
- gh3.9-1 mutante0
- Organ / stage
- roote0
- Gene
- GH3.9e0
- Outcome
- long-root length phenotypee0
- Direction
- long-roote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gh3.9-1 mutant along with GH3.9 RNAi lines and additional T-DNA insertions identified in the same region, revealed that GH3.9 might be involved in root development-related processes, as the mutants displayed a long-root length phenotype”
t0“Arabidopsis”
Claim · model-written summary
Exogenous IAA repressed GH3.9 transcript accumulation in seedlings.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seedlingse0
- Treatment
- exogenous IAA treatmente0
- Gene
- GH3.9e0
- Outcome
- GH3.9 transcript accumulatione0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GH3.9 transcript accumulation was repressed in seedlings treated with exogenous IAA treatment”
t0“Arabidopsis”
Claim · model-written summary
gh3.9-1 insertion and RNAi lines were sensitive to IAA-mediated root growth inhibition.
- Taxon
- ArabidopsisArabidopsist0
- Material
- gh3.9-1 T-DNA insertions and RNAi linese0
- Treatment
- IAAe0
- Gene
- gh3.9-1e0
- Outcome
- sensitivity to IAA-mediated root growth inhibitione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gh3.9-1 T-DNA insertions and RNAi lines showed sensitivity to IAA-mediated root growth inhibition”
t0“Arabidopsis”
Claim · model-written summary
gh3.9-1 insertion and RNAi lines resisted methyl jasmonate mediated root growth inhibition.
- Taxon
- ArabidopsisArabidopsist0
- Material
- gh3.9-1 T-DNA insertions and RNAi linese0
- Treatment
- Methyl Jasmonatee0
- Gene
- gh3.9-1e0
- Outcome
- resistance towards Methyl Jasmonate-mediated root growth inhibitione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gh3.9-1 T-DNA insertions and RNAi lines showed sensitivity to IAA-mediated root growth inhibition and resistance towards Methyl Jasmonate-mediated root growth inhibition”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that GH3.9 likely controls auxin activity through amino acid conjugation in roots.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rootse0
- Gene
- GH3.9e0
- Outcome
- auxin activitye0
- Qualifier
- through its amino acid conjugation activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GH3.9 in roots, where it likely controls auxin activity through its amino acid conjugation activity”
t0“Arabidopsis”
Claim · model-written summary
D-fbr7 was a novel FB1-resistant mutant.
- Taxon
- ArabidopsisArabidopsist0
- Material
- D-fbr7e0
- Outcome
- FB1-resistante0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D-fbr7 was identified as a novel FB1-resistant ( fbr) mutant”
t0“Arabidopsis”
Claim · model-written summary
D-fbr7 was defective in embryo development.
- Taxon
- ArabidopsisArabidopsist0
- Material
- D-fbr7e0
- Organ / stage
- embryoe0
- Outcome
- defective in embryo developmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D-fbr7 was identified as a novel FB1-resistant ( fbr) mutant that was subsequently also found to be defective in embryo development”
t0“Arabidopsis”
Claim · model-written summary
D-fbr7 resisted FB1-induced cell death at seedling and mature stages.
- Taxon
- ArabidopsisArabidopsist0
- Material
- D-fbr7e0
- Organ / stage
- seedling and mature development stagese0
- Outcome
- resistance of D-fbr7 to FB1-induced cell deathe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at both seedling and mature development stages, D-fbr7 displayed resistance to FB1 and FB1-induced senescence”
e1“resistance of D-fbr7 to FB1-induced cell death at different stages of plant development”
t0“Arabidopsis”
Claim · model-written summary
D-fbr7 resisted FB1-induced senescence at seedling and mature stages.
- Taxon
- ArabidopsisArabidopsist0
- Material
- D-fbr7e0
- Organ / stage
- seedling and mature development stagese0
- Outcome
- FB1-induced senescencee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at both seedling and mature development stages, D-fbr7 displayed resistance to FB1 and FB1-induced senescence”
t0“Arabidopsis”
Claim · model-written summary
Expression analysis implicated CLE19 in the fumonisin resistant phenotype.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CLE19e0
- Outcome
- fbr phenotypee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression analysis revealed that CLE19, member of CLAVATA3-LIKE (CLE) gene family is involved in causing the fbr phenotype”
t0“Arabidopsis”
Claim · model-written summary
Small RNAs adjacent to D-fbr7 may cause embryo lethality.
- Taxon
- ArabidopsisArabidopsist0
- Material
- D-fbr7e0
- Gene
- small RNAse0
- Outcome
- embryo lethalitye0
- Qualifier
- might be involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“small RNAs identified in the intergenic region adjacent to D-fbr7 might be involved in conferring embryo lethality”
t0“Arabidopsis”
Claim · model-written summary
Northern expression analysis validated the identified small RNAs.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- small RNAse0
- Outcome
- validated by northern expression analysise0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These small RNAs identified by web-based computational approaches were validated by northern expression analysis”
t0“Arabidopsis”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.