Abstract
Seed priming is a technique of controlled hydration and drying that results in more rapid germination when the seeds are reimbibed. Advancement of radicle meristem cells into the S and G2 phases of the cell cycle, increasing the percentage of nuclei having a 4C DNA content, has been reported to occur during priming. It has been suggested that the efficiency of priming is related to the accumulation of 4C nuclei in the radicle meristem, but the extent of cell cycle activity varied among different treatments and seed lots. A wide range of priming treatments across temperatures, water potentials and durations can be compared on a common basis using the hydrothermal priming time model. Flow cytometry was used to monitor cell cycle activity in a number of tomato (Lycopersicon esculentum Mill.) seed lots during priming in relation to the accumulation of hydrothermal priming time and the subsequent germination rate response. In some seed lots, the percentage of 4C nuclei in the radicle meristems prior to emergence increased in proportion to accumulated hydrothermal priming time, while in other lots, no increase in nuclear DNA content was detected. All lots, however, demonstrated rapid radicle emergence following priming. Thus, replicative DNA synthesis in radicle meristem nuclei often occurred during seed priming, but an increase in the percentage of 4C nuclei was not essential for germination advancement.
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Claims 4
Claim · model-written summary
Some tomato seed lots increased 4C nuclei with priming time.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- hydrothermal priming timee0
- Outcome
- percentage of 4C nucleie0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In some seed lots, the percentage of 4C nuclei in the radicle meristems prior to emergence increased in proportion to accumulated hydrothermal priming time”
e1“tomato (Lycopersicon esculentum Mill.) seed lots”
Claim · model-written summary
Other lots had no increase in nuclear DNA content.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- nuclear DNA contente0
- Direction
- no increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while in other lots, no increase in nuclear DNA content was detected”
e1“tomato (Lycopersicon esculentum Mill.) seed lots”
Claim · model-written summary
All lots emerged rapidly after priming.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- priminge0
- Outcome
- rapid radicle emergencee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All lots, however, demonstrated rapid radicle emergence following priming”
e1“tomato (Lycopersicon esculentum Mill.) seed lots”
Claim · model-written summary
Increasing 4C nuclei was not essential for germination advancement.
- Taxon
- tomatoSolanum lycopersicume1
- Outcome
- germination advancemente0
- Qualifier
- an increase in the percentage of 4C nuclei was not essentiale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in the percentage of 4C nuclei was not essential for germination advancement”
e1“tomato (Lycopersicon esculentum Mill.) seed lots”
The same questions in other papers
seed treatment · growth · Solanum lycopersicum (tomato)Positive and negative results
120 papers in the same direction as this paper · 10 in the opposite direction
- positive
- no effect
- no effect
127 papers more in this group
See all 131 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.