Abstract
Using in silico methods, several putative phytohormone-responsive cis-elements in the Oryza sativa non-symbiotic haemoglobin (NSHB) 1-4 and Arabidopsis thaliana NSHB1-2 promoters have been identified. An OsNSHB2 promoter::GUS reporter gene fusion shows tissue-specific expression in A. thaliana. GUS expression was observed in roots, the vasculature of young leaves, in flowers, and in the pedicel/stem junction. In transient assays, activity of the OsNSHB2 promoter was significantly up-regulated in the presence of the cytokinin, 6-benzylaminopurine (BA). Deletion analyses indicated that the full-length promoter was required for maximal trans-activation in the presence of cytokinin. Mutation of the single cytokinin-regulated ARR1-binding element abolished promoter activation in response to cytokinin. Constitutive expression of ARR1 under the control of the 35S cauliflower mosaic virus promoter enhanced wild-type OsNSHB2 promoter activity, but had no effect on the activity of the mutated promoter in the absence of cytokinin. However, overexpression of ARR1 in the presence of cytokinin resulted in super-activation of the wild-type promoter. The mutated promoter was only moderately activated in the presence of cytokinin and ARR1, indicating that the OsNSHB2 promoter can be regulated by the ARR1 protein, but requires other cytokinin-induced factors for optimal activation. This is the first report that identifies a trans-acting factor involved in the activation of a NSHB gene.
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Claims 12
Claim · model-written summary
Several putative phytohormone-responsive cis-elements were identified in the rice NSHB promoters by in silico methods.
- Taxon
- Oryza sativa
Oryza sativat0 - Outcome
- putative phytohormone-responsive cis-elementse0
- Qualifier
- Using in silico methodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using in silico methods, several putative phytohormone-responsive cis-elements in the Oryza sativa non-symbiotic haemoglobin (NSHB) 1-4 and Arabidopsis thaliana NSHB1-2 promoters have been identified”
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
Claim · model-written summary
The OsNSHB2 promoter fused to GUS is expressed in roots, young leaf vasculature and flowers of Arabidopsis.
- Taxon
- Oryza sativaOryza sativat0
- Material
- OsNSHB2 promoter::GUS reporter gene fusione1
- Organ / stage
- roots, the vasculature of young leaves, in flowerse2
- Outcome
- GUS expressione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e1“An OsNSHB2 promoter::GUS reporter gene fusion shows tissue-specific expression in A. thaliana”
e2“GUS expression was observed in roots, the vasculature of young leaves, in flowers, and in the pedicel/stem junction”
Claim · model-written summary
The OsNSHB2 promoter::GUS fusion shows GUS expression at the pedicel/stem junction.
- Taxon
- Oryza sativaOryza sativat0
- Organ / stage
- pedicel/stem junctione2
- Outcome
- GUS expressione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e2“GUS expression was observed in roots, the vasculature of young leaves, in flowers, and in the pedicel/stem junction”
Claim · model-written summary
Cytokinin significantly up-regulated OsNSHB2 promoter activity in transient assays.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- cytokinin, 6-benzylaminopurine (BA)e3
- Outcome
- activity of the OsNSHB2 promotere3
- Direction
- up-regulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e3“activity of the OsNSHB2 promoter was significantly up-regulated in the presence of the cytokinin, 6-benzylaminopurine (BA)”
Claim · model-written summary
The full-length OsNSHB2 promoter gave maximal trans-activation with cytokinin compared with deletion analyses.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- Deletion analysese4
- Outcome
- maximal trans-activatione4
- Direction
- maximale4
- Qualifier
- in the presence of cytokinine4
- Comparator
- full-length promotere4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e4“Deletion analyses indicated that the full-length promoter was required for maximal trans-activation in the presence of cytokinin”
Claim · model-written summary
Mutation of the ARR1-binding element abolished OsNSHB2 promoter activation in response to cytokinin.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- Mutation of the single cytokinin-regulated ARR1-binding elemente5
- Gene
- ARR1e5
- Outcome
- promoter activation in response to cytokinine5
- Direction
- abolishede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e5“Mutation of the single cytokinin-regulated ARR1-binding element abolished promoter activation in response to cytokinin”
Claim · model-written summary
Constitutive ARR1 expression enhanced wild-type OsNSHB2 promoter activity.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- Constitutive expression of ARR1 under the control of the 35S cauliflower mosaic virus promotere6
- Gene
- ARR1e6
- Outcome
- wild-type OsNSHB2 promoter activitye6
- Direction
- enhancede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e6“Constitutive expression of ARR1 under the control of the 35S cauliflower mosaic virus promoter enhanced wild-type OsNSHB2 promoter activity”
Claim · model-written summary
Constitutive ARR1 expression had no effect on the mutated promoter activity without cytokinin.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- Constitutive expression of ARR1 under the control of the 35S cauliflower mosaic virus promotere6
- Gene
- ARR1e6
- Outcome
- activity of the mutated promotere7
- Direction
- no effecte7
- Qualifier
- in the absence of cytokinine7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e6“Constitutive expression of ARR1 under the control of the 35S cauliflower mosaic virus promoter enhanced wild-type OsNSHB2 promoter activity”
e7“had no effect on the activity of the mutated promoter in the absence of cytokinin”
Claim · model-written summary
Overexpression of ARR1 with cytokinin super-activated the wild-type OsNSHB2 promoter.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- overexpression of ARR1 in the presence of cytokinine1
- Gene
- ARR1e1
- Outcome
- the wild-type promotere1
- Direction
- super-activatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e1“overexpression of ARR1 in the presence of cytokinin resulted in super-activation of the wild-type promoter”
Claim · model-written summary
The mutated OsNSHB2 promoter was only moderately activated by cytokinin and ARR1.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- cytokinin and ARR1e2
- Outcome
- mutated promotere2
- Direction
- only moderately activatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e2“The mutated promoter was only moderately activated in the presence of cytokinin and ARR1”
Claim · model-written summary
The OsNSHB2 promoter is regulated by ARR1 but requires other cytokinin-induced factors for optimal activation.
- Taxon
- Oryza sativaOryza sativat0
- Gene
- ARR1 proteine2
- Outcome
- optimal activatione2
- Qualifier
- requires other cytokinin-induced factorse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e2“the OsNSHB2 promoter can be regulated by the ARR1 protein, but requires other cytokinin-induced factors for optimal activation”
Claim · model-written summary
This study is the first report of a trans-acting factor involved in activating an NSHB gene.
- Taxon
- Oryza sativaOryza sativat0
- Gene
- NSHB genee2
- Outcome
- trans-acting factor involved in the activatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1”
e2“This is the first report that identifies a trans-acting factor involved in the activation of a NSHB gene”
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.