Abstract
Perception of pathogen (or microbe)-associated molecular patterns (PAMPs/MAMPs) by pattern recognition receptors (PRRs) is a key component of plant innate immunity. The Arabidopsis PRR EF-Tu receptor (EFR) recognizes the bacterial PAMP elongation factor Tu (EF-Tu) and its derived peptide elf18. Previous work revealed that transgenic expression of AtEFR in Solanaceae confers elf18 responsiveness and broad-spectrum bacterial disease resistance. In this study, we developed a set of bioassays to study the activation of PAMP-triggered immunity (PTI) in wheat. We generated transgenic wheat (Triticum aestivum) plants expressing AtEFR driven by the constitutive rice actin promoter and tested their response to elf18. We show that transgenic expression of AtEFR in wheat confers recognition of elf18, as measured by the induction of immune marker genes and callose deposition. When challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzae, transgenic EFR wheat lines had reduced lesion size and bacterial multiplication. These results demonstrate that AtEFR can be transferred successfully from dicot to monocot species, further revealing that immune signalling pathways are conserved across these distant phyla. As novel PRRs are identified, their transfer between plant families represents a useful strategy for enhancing resistance to pathogens in crops.
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Claims 4
Claim · model-written summary
AtEFR expression in wheat enabled elf18 recognition, indicated by immune marker gene induction.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- transgenic expression of AtEFRe0
- Gene
- AtEFRe0
- Outcome
- induction of immune marker genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic expression of AtEFR in wheat confers recognition of elf18, as measured by the induction of immune marker genes and callose deposition”
Claim · model-written summary
AtEFR expression in wheat enabled elf18 recognition, indicated by callose deposition.
- Taxon
- wheatTriticum aestivume0
- Treatment
- transgenic expression of AtEFRe0
- Gene
- AtEFRe0
- Outcome
- callose depositione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic expression of AtEFR in wheat confers recognition of elf18, as measured by the induction of immune marker genes and callose deposition”
Claim · model-written summary
EFR wheat lines had smaller lesions after Pseudomonas syringae pv. oryzae challenge.
- Taxon
- wheatTriticum aestivume0
- Material
- transgenic EFR wheat linese0
- Treatment
- challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzaee0
- Interactor
- Pseudomonas syringae pv. oryzaePseudomonas coronafaciens pv. oryzaee0pathogen
- Outcome
- lesion sizee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzae, transgenic EFR wheat lines had reduced lesion size and bacterial multiplication”
Claim · model-written summary
EFR wheat lines had reduced bacterial multiplication after Pseudomonas syringae pv. oryzae challenge.
- Taxon
- wheatTriticum aestivume0
- Material
- transgenic EFR wheat linese0
- Treatment
- challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzaee0
- Interactor
- Pseudomonas syringae pv. oryzaePseudomonas coronafaciens pv. oryzaee0pathogen
- Outcome
- bacterial multiplicatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When challenged with the cereal bacterial pathogen Pseudomonas syringae pv. oryzae, transgenic EFR wheat lines had reduced lesion size and bacterial multiplication”
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