Abstract
Peroxisomes house critical metabolic reactions that are essential for seedling development. As seedlings mature, metabolic requirements change, and peroxisomal contents are remodeled. The resident peroxisomal protease LON2 is positioned to degrade obsolete or damaged peroxisomal proteins, but data supporting such a role in plants have remained elusive. Arabidopsis thaliana lon2 mutants display defects in peroxisomal metabolism and matrix protein import but appear to degrade matrix proteins normally. To elucidate LON2 functions, we executed a forward-genetic screen for lon2 suppressors, which revealed multiple mutations in key autophagy genes. Disabling core autophagy-related gene (ATG) products prevents autophagy, a process through which cytosolic constituents, including organelles, can be targeted for vacuolar degradation. We found that atg2, atg3, and atg7 mutations suppressed lon2 defects in auxin metabolism and matrix protein processing and rescued the abnormally large size and small number of lon2 peroxisomes. Moreover, analysis of lon2 atg mutants uncovered an apparent role for LON2 in matrix protein turnover. Our data suggest that LON2 facilitates matrix protein degradation during peroxisome content remodeling, provide evidence for the existence of pexophagy in plants, and indicate that peroxisome destruction via autophagy is enhanced when LON2 is absent.
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Claims 6
Claim · model-written summary
Mutations in autophagy genes were identified as lon2 suppressors.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- autophagy genese0
- Outcome
- lon2 suppressorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a forward-genetic screen for lon2 suppressors, which revealed multiple mutations in key autophagy genes.”
t0“Disrupting Autophagy Restores Peroxisome Function to anArabidopsis lon2Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation”
Claim · model-written summary
atg2, atg3 and atg7 mutations suppressed lon2 auxin metabolism and matrix protein processing defects.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- atg2, atg3, and atg7 mutationse0
- Gene
- atg2, atg3, and atg7e0
- Outcome
- lon2 defects in auxin metabolism and matrix protein processinge0
- Direction
- suppressede0
- Comparator
- lon2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“atg2, atg3, and atg7 mutations suppressed lon2 defects in auxin metabolism and matrix protein processing”
t0“Disrupting Autophagy Restores Peroxisome Function to anArabidopsis lon2Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation”
Claim · model-written summary
atg mutations rescued abnormal lon2 peroxisome size and number.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- atg2, atg3, and atg7 mutationse1
- Outcome
- abnormally large size and small number of lon2 peroxisomese0
- Direction
- rescuede0
- Comparator
- lon2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rescued the abnormally large size and small number of lon2 peroxisomes.”
t0“Disrupting Autophagy Restores Peroxisome Function to anArabidopsis lon2Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation”
e1“atg2, atg3, and atg7 mutations suppressed lon2 defects in auxin metabolism and matrix protein processing and rescued the abnormally large size and small number of lon2 peroxisomes.”
Claim · model-written summary
LON2 appears to mediate matrix protein turnover.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- LON2e0
- Outcome
- matrix protein turnovere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of lon2 atg mutants uncovered an apparent role for LON2 in matrix protein turnover.”
t0“Disrupting Autophagy Restores Peroxisome Function to anArabidopsis lon2Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation”
Claim · model-written summary
LON2 facilitates matrix protein degradation during peroxisome remodeling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- LON2e0
- Outcome
- matrix protein degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LON2 facilitates matrix protein degradation during peroxisome content remodeling”
t0“Disrupting Autophagy Restores Peroxisome Function to anArabidopsis lon2Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation”
Claim · model-written summary
Autophagic peroxisome destruction is enhanced without LON2.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- LON2e0
- Outcome
- peroxisome destruction via autophagye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peroxisome destruction via autophagy is enhanced when LON2 is absent.”
t0“Disrupting Autophagy Restores Peroxisome Function to anArabidopsis lon2Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation”
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