Abstract
beta-Mannosidase and endo-beta-mannanase are involved in the mobilization of the mannan-containing cell walls of the tomato seed endosperm. The activities of both enzymes increase in a similar temporal manner in the micropylar and lateral endosperm during and following germination. This increase in enzyme activities in the micropylar endosperm is not markedly reduced in seeds imbibed in abscisic acid although, in the lateral endosperm, endo-beta-mannanase activity is more suppressed by this inhibitor than is the activity of beta-mannosidase. Gibberellin-deficient (gib-1) mutants of tomato do not germinate unless imbibed in gibberellin; low beta-mannosidase activity, and no endo-beta-mannanase activity is present in seeds imbibed in water, but both enzymes increase strongly in activity in the seeds imbibed in the growth regulator. For production of full activity of both beta-mannosidase and endo-beta-mannanase in the endosperm, this tissue must be in contact with the embryo for at least the first 6 h of imbibition, which is indicative of a stimulus diffusing from the embryo to the endosperm during this time. These results suggest some correlation between the activities of beta-mannosidase and endo-beta-mannanase, particularly in the micropylar endosperm, in populations of tomato seeds imbibed in water, abscisic acid and gibberellin. However, when individual micropylar endosperm parts are used to examine the effect of the growth regulators and of imbibition in water on the production of the two enzymes, it is apparent that within these individual seed parts there may be large differences in the amount of enzyme activity present. Micropylar endosperms with high endo-beta-mannanase activity do not necessarily have high beta-mannosidase activity, and vice versa, which is indicative of a lack of co-ordination of the activities of these two enzymes within individuals of a population.
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Claims 13
Claim · model-written summary
Both enzyme activities increase similarly through germination in micropylar and lateral endosperm.
- Organ / stage
- micropylar and lateral endosperme0
- Outcome
- activities of both enzymese0
- Direction
- increasee0
Not reportedTaxon
Verbatim
e0“The activities of both enzymes increase in a similar temporal manner in the micropylar and lateral endosperm during and following germination.”
Claim · model-written summary
Abscisic acid does not markedly reduce the micropylar enzyme activity increase.
- Organ / stage
- micropylar endosperme0
- Treatment
- seeds imbibed in abscisic acide0
- Outcome
- increase in enzyme activitiese0
- Direction
- not markedly reducede0
Not reportedTaxonComparator
Verbatim
e0“This increase in enzyme activities in the micropylar endosperm is not markedly reduced in seeds imbibed in abscisic acid”
Claim · model-written summary
In lateral endosperm, abscisic acid suppresses endo-beta-mannanase more than beta-mannosidase.
- Organ / stage
- lateral endosperme0
- Treatment
- this inhibitore0
- Gene
- endo-beta-mannanasee0
- Outcome
- endo-beta-mannanase activitye0
- Direction
- more suppressede0
- Comparator
- beta-mannosidasee0
Not reportedTaxon
Verbatim
e0“in the lateral endosperm, endo-beta-mannanase activity is more suppressed by this inhibitor than is the activity of beta-mannosidase.”
Claim · model-written summary
Gibberellin-deficient gib-1 tomato mutants fail to germinate without gibberellin.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- gib-1e0
- Outcome
- do not germinatee0
- Qualifier
- unless imbibed in gibberelline0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gibberellin-deficient (gib-1) mutants of tomato do not germinate unless imbibed in gibberellin”
Claim · model-written summary
Gib-1 seeds imbibed in water have low beta-mannosidase activity.
- Organ / stage
- seedse0
- Treatment
- imbibed in watere0
- Gene
- beta-mannosidasee0
- Outcome
- low beta-mannosidase activitye0
Not reportedTaxon
Verbatim
e0“low beta-mannosidase activity, and no endo-beta-mannanase activity is present in seeds imbibed in water”
Claim · model-written summary
Gib-1 seeds imbibed in water have no endo-beta-mannanase activity.
- Organ / stage
- seedse0
- Treatment
- imbibed in watere0
- Gene
- endo-beta-mannanasee0
- Outcome
- no endo-beta-mannanase activitye0
Not reportedTaxon
Verbatim
e0“low beta-mannosidase activity, and no endo-beta-mannanase activity is present in seeds imbibed in water”
Claim · model-written summary
Both enzyme activities increase strongly in gib-1 seeds given the growth regulator.
- Organ / stage
- seedse0
- Treatment
- the growth regulatore0
- Outcome
- both enzymese0
- Direction
- increase stronglye0
Not reportedTaxonComparator
Verbatim
e0“both enzymes increase strongly in activity in the seeds imbibed in the growth regulator.”
Claim · model-written summary
Full activity of both endosperm enzymes requires embryo contact for the first six hours of imbibition.
- Organ / stage
- endosperme0
- Outcome
- full activity of both beta-mannosidase and endo-beta-mannanasee0
- Qualifier
- in contact with the embryo for at least the first 6 h of imbibitione0
Not reportedTaxon
Verbatim
e0“For production of full activity of both beta-mannosidase and endo-beta-mannanase in the endosperm, this tissue must be in contact with the embryo for at least the first 6 h of imbibition”
Claim · model-written summary
The authors infer a signal diffuses from embryo to endosperm during early imbibition.
- Organ / stage
- embryoe0
- Outcome
- a stimulus diffusing from the embryo to the endosperme0
- Qualifier
- during this timee0
Not reportedTaxon
Verbatim
e0“a stimulus diffusing from the embryo to the endosperm during this time.”
Claim · model-written summary
Across tomato seed populations, beta-mannosidase and endo-beta-mannanase activities show some correlation.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- micropylar endosperme0
- Gene
- beta-mannosidasee0
- Outcome
- activities of beta-mannosidase and endo-beta-mannanasee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some correlation between the activities of beta-mannosidase and endo-beta-mannanase, particularly in the micropylar endosperm, in populations of tomato seeds imbibed in water, abscisic acid and gibberellin.”
Claim · model-written summary
Individual micropylar endosperms varied greatly in enzyme activity.
Not reportedTaxonDirection
Verbatim
e0“within these individual seed parts there may be large differences in the amount of enzyme activity present.”
Claim · model-written summary
High endo-beta-mannanase activity in an individual micropylar endosperm did not necessarily accompany high beta-mannosidase activity.
Not reportedTaxonDirection
Verbatim
e0“Micropylar endosperms with high endo-beta-mannanase activity do not necessarily have high beta-mannosidase activity, and vice versa”
Claim · model-written summary
The authors infer that enzyme activities are not coordinated within individual seeds.
- Outcome
- lack of co-ordination of the activities of these two enzymese0
- Qualifier
- within individuals of a populatione0
Not reportedTaxon
Verbatim
e0“a lack of co-ordination of the activities of these two enzymes within individuals of a population.”
The same questions in other papers
class · physiology: enzyme activity · Solanum lycopersicum (tomato)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.