Abstract
The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers. Because radioactive assays alone cannot distinguish IBA from its metabolites, the detected transport from applied [3H]IBA may have resulted from the transport of IBA metabolites, including IAA. To test this hypothesis, we used a mass spectrometry-based method to quantify the transport of IBA in Arabidopsis hypocotyls by following the movement of [13C1]IBA and the [13C1]IAA derived from [13C1]IBA. We also assayed [13C6]IAA transport in a parallel control experiment. We found that the amount of transported [13C1]IBA was dramatically lower than [13C6]IAA, and the IBA transport was not reduced by the auxin transport inhibitor N-1-naphthylphthalamic acid. Significant amounts of the applied [13C1]IBA were converted to [13C1]IAA during transport, but [13C1]IBA transport was independent of IBA-to-IAA conversion. We also found that most of the [13C1]IBA was converted to ester-linked [13C1]IBA at the apical end of hypocotyls, and ester-linked [13C1]IBA was also found in the basal end at a level higher than free [13C1]IBA. In contrast, most of the [13C6]IAA was converted to amide-linked [13C6]IAA at the apical end of hypocotyls, but very little conjugated [13C6]IAA was found in the basal end. Our results demonstrate that the polar transport of IBA is much lower than IAA in Arabidopsis hypocotyls, and the transport mechanism is distinct from IAA transport. These experiments also establish a method for quantifying the movement of small molecules in plants using stable isotope labeling.
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Claims 11
Claim · model-written summary
Transported [13C1]IBA amount was dramatically lower than transported [13C6]IAA in Arabidopsis hypocotyls.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- [13C1]IBAe1
- Outcome
- amount of transportede1
- Direction
- lowere1
- Comparator
- [13C6]IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e1“We found that the amount of transported [13C1]IBA was dramatically lower than [13C6]IAA”
Claim · model-written summary
The auxin transport inhibitor N-1-naphthylphthalamic acid did not reduce IBA transport in Arabidopsis hypocotyls.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- auxin transport inhibitor N-1-naphthylphthalamic acide2
- Outcome
- IBA transporte2
- Direction
- not reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e2“the IBA transport was not reduced by the auxin transport inhibitor N-1-naphthylphthalamic acid”
Claim · model-written summary
Significant amounts of applied [13C1]IBA were converted to [13C1]IAA during transport in Arabidopsis hypocotyls.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- converted to [13C1]IAAe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e3“Significant amounts of the applied [13C1]IBA were converted to [13C1]IAA during transport”
Claim · model-written summary
[13C1]IBA transport was independent of IBA-to-IAA conversion.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- [13C1]IBA transporte4
- Direction
- independent ofe4
- Comparator
- IBA-to-IAA conversione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e4“[13C1]IBA transport was independent of IBA-to-IAA conversion”
Claim · model-written summary
Most applied [13C1]IBA was converted to ester-linked [13C1]IBA at the apical end of hypocotyls.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- apical end of hypocotylse5
- Outcome
- ester-linked [13C1]IBAe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e5“most of the [13C1]IBA was converted to ester-linked [13C1]IBA at the apical end of hypocotyls”
Claim · model-written summary
Ester-linked [13C1]IBA in the basal end of hypocotyls was higher than free [13C1]IBA.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- basal ende6
- Outcome
- ester-linked [13C1]IBAe6
- Direction
- highere6
- Comparator
- free [13C1]IBAe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e6“ester-linked [13C1]IBA was also found in the basal end at a level higher than free [13C1]IBA”
Claim · model-written summary
Most [13C6]IAA was converted to amide-linked [13C6]IAA at the apical end of hypocotyls.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- apical end of hypocotylse7
- Outcome
- converted to amide-linked [13C6]IAAe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e7“most of the [13C6]IAA was converted to amide-linked [13C6]IAA at the apical end of hypocotyls”
Claim · model-written summary
Very little conjugated [13C6]IAA was found in the basal end of Arabidopsis hypocotyls.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- basal ende1
- Outcome
- conjugated [13C6]IAAe1
- Qualifier
- very littlee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e1“very little conjugated [13C6]IAA was found in the basal end”
Claim · model-written summary
Polar transport of IBA is much lower than that of IAA in Arabidopsis hypocotyls.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- polar transport of IBAe1
- Direction
- lowere1
- Comparator
- IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e1“the polar transport of IBA is much lower than IAA in Arabidopsis hypocotyls”
Claim · model-written summary
The transport mechanism of IBA is distinct from IAA transport.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- transport mechanisme1
- Qualifier
- distinct from IAA transporte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e1“the transport mechanism is distinct from IAA transport”
Claim · model-written summary
Stable isotope labeling establishes a method for quantifying the movement of small molecules in Arabidopsis hypocotyls, checked against a parallel control experiment.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- quantifying the movement of small moleculese1
- Comparator
- parallel control experimente2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polar transport of the natural auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) has been described in Arabidopsis (Arabidopsis thaliana) hypocotyls using radioactive tracers”
e1“These experiments also establish a method for quantifying the movement of small molecules in plants using stable isotope labeling”
e2“We also assayed [13C6]IAA transport in a parallel control experiment”
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