Abstract
Protein synthesis is very sensitive to NaCl. However, the molecular targets responsible for this sensitivity have not been described. A cDNA library of the halotolerant plant sugar beet was functionally screened in a sodium-sensitive yeast strain. We obtained a cDNA clone (BveIF1A) encoding the eukaryotic translation initiation factor eIF1A. BveIF1A was able to partially complement the yeast eIF1A-deficient strain. Overexpression of the sugar beet eIF1A specifically increased the sodium and lithium salt tolerance of yeast. This phenotype was not accompanied by changes in sodium or potassium homeostasis. Under salt stress conditions, yeast cells expressing BveIF1A presented a higher rate of amino acid incorporation into proteins than control cells. In an in vitro protein synthesis system from wheat germ, the BveIF1A recombinant protein improved translation in the presence of NaCl. Finally, transgenic Arabidopsis plants expressing BveIF1A exhibited increased tolerance to NaCl. These results suggest that the translation initiation factor eIF1A is an important determinant of sodium tolerance in yeast and plants.
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Claims 6
Claim · model-written summary
Overexpression of sugar beet eIF1A increased sodium and lithium salt tolerance of yeast.
- Taxon
- sugar beet
Beta vulgaris subsp. vulgarise0 - Material
- yeaste1
- Treatment
- Overexpression of the sugar beet eIF1Ae1
- Gene
- sugar beet eIF1Ae1
- Outcome
- sodium and lithium salt tolerancee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA library of the halotolerant plant sugar beet was functionally screened in a sodium-sensitive yeast strain”
e1“Overexpression of the sugar beet eIF1A specifically increased the sodium and lithium salt tolerance of yeast”
Claim · model-written summary
The eIF1A-associated increase in salt tolerance was not accompanied by changes in sodium or potassium homeostasis.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Outcome
- sodium or potassium homeostasise2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA library of the halotolerant plant sugar beet was functionally screened in a sodium-sensitive yeast strain”
e2“This phenotype was not accompanied by changes in sodium or potassium homeostasis”
Claim · model-written summary
Under salt stress, yeast cells expressing BveIF1A had a higher rate of amino acid incorporation into proteins than control cells.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Material
- yeast cells expressing BveIF1Ae3
- Treatment
- Under salt stress conditionse3
- Gene
- BveIF1Ae3
- Outcome
- rate of amino acid incorporation into proteinse3
- Direction
- highere3
- Comparator
- control cellse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA library of the halotolerant plant sugar beet was functionally screened in a sodium-sensitive yeast strain”
e3“Under salt stress conditions, yeast cells expressing BveIF1A presented a higher rate of amino acid incorporation into proteins than control cells”
Claim · model-written summary
The BveIF1A recombinant protein improved translation in the presence of NaCl in a wheat germ in vitro system.
- Taxon
- wheat
Triticum aestivume4 - Treatment
- in the presence of NaCle4
- Gene
- BveIF1A recombinant proteine4
- Outcome
- translatione4
- Direction
- improvede4
- Setting
- in vitro protein synthesis systeme4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“In an in vitro protein synthesis system from wheat germ, the BveIF1A recombinant protein improved translation in the presence of NaCl”
Claim · model-written summary
Transgenic Arabidopsis plants expressing BveIF1A had increased tolerance to NaCl.
- Taxon
- Arabidopsis
Arabidopsise5 - Material
- transgenic Arabidopsis plantse5
- Treatment
- NaCle5
- Gene
- BveIF1Ae5
- Outcome
- tolerancee5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA library of the halotolerant plant sugar beet was functionally screened in a sodium-sensitive yeast strain”
e5“transgenic Arabidopsis plants expressing BveIF1A exhibited increased tolerance to NaCl”
Claim · model-written summary
BveIF1A partially complemented the yeast eIF1A-deficient strain.
- Taxon
- sugar beetBeta vulgaris subsp. vulgarise0
- Gene
- BveIF1Ae6
- Outcome
- partially complemente6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA library of the halotolerant plant sugar beet was functionally screened in a sodium-sensitive yeast strain”
e6“BveIF1A was able to partially complement the yeast eIF1A-deficient strain”
The same questions in other papers
salinity · molecular: metabolites · Beta vulgaris (beet)Positive and negative results
6 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.