Abstract
We performed high-throughput screening using the potato virus X (PVX) system to overexpress Nicotiana benthamiana genes in planta and identify positive regulators of cell death. This screening identified NbCD1, a novel class II ethylene-responsive element binding factor (ERF), as a potent inducer of the hypersensitive response (HR)-like cell death. NbCD1 expression was induced by treatments with INF1 elicitor and a non-host pathogen Pseudomonas cichorii. NbCD1 exhibited transcriptional repressor activity through its EAR motif, and this motif was necessary for NbCD1 to cause cell death. We identified 58 genes that displayed altered transcription following NbCD1 overexpression. NbCD1 overexpression downregulated the expression of HSR203, a negative regulator of hypersensitive death. Conditional expression of NbCD1 in Arabidopsis also caused cell death, indicating that NbCD1 downstream cascades are conserved in dicot plants. To further confirm the role of NbCD1 in defense, we used virus-induced gene silencing to demonstrate that NbCD1 is required for non-host resistance of N. benthamiana to the bacterial pathogen P. cichorii. Our data point to a model of transcriptional regulatory cascades. NbCD1 positively regulates cell death and contributes to non-host resistance, possibly by downregulating the expression of other defense response genes.
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Claims 9
Claim · model-written summary
This screening identified NbCD1, a novel class II ethylene-responsive element binding factor (ERF), as a potent inducer of the hypersensitive response (HR)-like cell death.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- NbCD1e1
- Outcome
- hypersensitive response (HR)-like cell deathe1
- Value
- NbCD1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana genes in planta”
e1“This screening identified NbCD1, a novel class II ethylene-responsive element binding factor (ERF), as a potent inducer of the hypersensitive response (HR)-like cell death”
Claim · model-written summary
NbCD1 expression was induced by treatments with INF1 elicitor.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- treatments with INF1 elicitore1
- Gene
- NbCD1e1
- Outcome
- NbCD1 expressione1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana genes in planta”
e1“NbCD1 expression was induced by treatments with INF1 elicitor”
Claim · model-written summary
NbCD1 exhibited transcriptional repressor activity through its EAR motif.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Gene
- NbCD1e1
- Outcome
- transcriptional repressor activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana genes in planta”
e1“NbCD1 exhibited transcriptional repressor activity through its EAR motif”
Claim · model-written summary
this motif was necessary for NbCD1 to cause cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Gene
- NbCD1e1
- Outcome
- cell deathe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana genes in planta”
e1“this motif was necessary for NbCD1 to cause cell death”
Claim · model-written summary
We identified 58 genes that displayed altered transcription following NbCD1 overexpression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Gene
- NbCD1e1
- Outcome
- genes that displayed altered transcriptione1
- Value
- 58 genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana genes in planta”
e1“We identified 58 genes that displayed altered transcription following NbCD1 overexpression”
Claim · model-written summary
NbCD1 overexpression downregulated the expression of HSR203.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- NbCD1 overexpressione1
- Gene
- NbCD1e1
- Outcome
- expression of HSR203e1
- Direction
- downregulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana genes in planta”
e1“NbCD1 overexpression downregulated the expression of HSR203”
Claim · model-written summary
Pseudomonas cichorii induced NbCD1 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- Pseudomonas cichoriie1
- Interactor
- Pseudomonas cichorii
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Pseudomonas cichoriie1pathogen - Gene
- NbCD1e1
- Outcome
- NbCD1 expressione1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Nicotiana benthamiana genes in planta”
e1“NbCD1 expression was induced by treatments with INF1 elicitor and a non-host pathogen Pseudomonas cichorii”
Claim · model-written summary
Conditional NbCD1 expression caused cell death in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- Conditional expression of NbCD1e1
- Gene
- NbCD1e1
- Outcome
- cell deathe1
- Direction
- causede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Conditional expression of NbCD1 in Arabidopsis also caused cell death”
Claim · model-written summary
NbCD1 was required for non-host resistance to P. cichorii.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Interactor
- P. cichoriiPseudomonas cichoriie1pathogen
- Gene
- NbCD1e1
- Outcome
- non-host resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“NbCD1 is required for non-host resistance of N. benthamiana to the bacterial pathogen P. cichorii”
The same questions in other papers
resistance inducer · molecular: expression · Nicotiana benthamianaResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.