Abstract
To study the impact of translational regulation during heavy metal poisoning, Arabidopsis thaliana cell cultures were submitted to sublethal cadmium stress. At the concentration used, cadmium had a minimal impact on the growth of the culture but induced an accumulation of high molecular weight polysomes without de novo production of new ribosomes together with a reduction of protein synthesis. In addition, cadmium stress induces phosphorylation of eukaryotic initiation factor 2α by GCN2 and, in planta, gcn2 mutants are more sensitive to cadmium stress, suggesting a role for this translational regulation mechanism in the response to cadmium stress. Microarray analysis of total and polysomal RNAs in control and cadmium-treated cells reveals a large class of genes for which a variation in total RNA abundance is not linked to a variation in polysomal loading, suggesting that transcription and translation are uncoupled and that these genes are not recruited at the initiation step of translation.
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Claims 6
Claim · model-written summary
Sublethal cadmium had a minimal impact on the growth of Arabidopsis cell cultures.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- sublethal cadmium stresse0
- Outcome
- growth of the culturee1
- Direction
- minimal impacte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In Arabidopsis thaliana”
e0“Arabidopsis thaliana cell cultures were submitted to sublethal cadmium stress”
e1“At the concentration used, cadmium had a minimal impact on the growth of the culture but induced an accumulation of high molecular weight polysomes without de novo production of new ribosomes together with a reduction of protein synthesis”
Claim · model-written summary
Sublethal cadmium induced an accumulation of high molecular weight polysomes without de novo production of new ribosomes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- sublethal cadmium stresse0
- Outcome
- high molecular weight polysomese1
- Direction
- an accumulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In Arabidopsis thaliana”
e0“Arabidopsis thaliana cell cultures were submitted to sublethal cadmium stress”
e1“At the concentration used, cadmium had a minimal impact on the growth of the culture but induced an accumulation of high molecular weight polysomes without de novo production of new ribosomes together with a reduction of protein synthesis”
Claim · model-written summary
Sublethal cadmium reduced protein synthesis in Arabidopsis cell cultures.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- sublethal cadmium stresse0
- Outcome
- protein synthesise1
- Direction
- a reductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In Arabidopsis thaliana”
e0“Arabidopsis thaliana cell cultures were submitted to sublethal cadmium stress”
e1“At the concentration used, cadmium had a minimal impact on the growth of the culture but induced an accumulation of high molecular weight polysomes without de novo production of new ribosomes together with a reduction of protein synthesis”
Claim · model-written summary
Cadmium stress induced phosphorylation of eukaryotic initiation factor 2α by GCN2.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cadmium stresse2
- Gene
- GCN2e2
- Outcome
- phosphorylation of eukaryotic initiation factor 2αe2
- Direction
- inducese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In Arabidopsis thaliana”
e2“cadmium stress induces phosphorylation of eukaryotic initiation factor 2α by GCN2”
Claim · model-written summary
gcn2 mutants were more sensitive to cadmium stress in planta.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- gcn2 mutantse3
- Treatment
- cadmium stresse3
- Outcome
- sensitive to cadmium stresse3
- Direction
- moree3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In Arabidopsis thaliana”
e3“in planta, gcn2 mutants are more sensitive to cadmium stress”
Claim · model-written summary
For many genes, variation in total RNA abundance was not linked to variation in polysomal loading in cadmium-treated cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cadmium-treated cellse4
- Outcome
- polysomal loadinge4
- Direction
- is not linkede4
- Comparator
- controle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In Arabidopsis thaliana”
e4“Microarray analysis of total and polysomal RNAs in control and cadmium-treated cells reveals a large class of genes for which a variation in total RNA abundance is not linked to a variation in polysomal loading”
The same questions in other papers
heavy metal · growth · Arabidopsis thaliana (thale cress)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.