Abstract
Somatic embryogenesis is an obvious experimental evidence of totipotency, and is used as a model system for studying the mechanisms of de-differentiation and re-differentiation of plant cells. Although Arabidopsis is widely used as a model plant for genetic and molecular biological studies, there is no available tissue culture system for inducing somatic embryogenesis from somatic cells in this plant. We established a new tissue culture system using stress treatment to induce somatic embryogenesis in Arabidopsis. In this system, stress treatment induced formation of somatic embryos from shoot-apical-tip and floral-bud explants. The somatic embryos grew into young plantlets with normal morphology, including cotyledons, hypocotyls, and roots, and some embryo-specific genes (ABI3 and FUS3) were expressed in these embryos. Several stresses (osmotic, heavy metal ion, and dehydration stress) induced somatic embryogenesis, but the optimum stress treatment differed between different stressors. When we used mannitol to cause osmotic stress, the optimal conditions for somatic embryogenesis were 6-9 h of culture on solid B5 medium containing 0.7 m mannitol, after which the explants were transferred to B5 medium containing 2,4-dichlorophenoxyacetic acid (2,4-D, 4.5 microm), but no mannitol. Using this tissue culture system, we induced somatic embryogenesis in three major ecotypes of Arabidopsis thaliana-Ws, Col, and Ler.
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Claims 13
Claim · model-written summary
Stress treatment induced somatic embryos from shoot apical tip explants.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- shoot-apical-tipe0
- Treatment
- stress treatmente0
- Outcome
- formation of somatic embryose0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress treatment induced formation of somatic embryos from shoot-apical-tip and floral-bud explants”
t0“Arabidopsis thaliana”
Claim · model-written summary
Stress treatment induced somatic embryos from floral bud explants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- floral-bud explantse0
- Treatment
- stress treatmente0
- Outcome
- formation of somatic embryose0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress treatment induced formation of somatic embryos from shoot-apical-tip and floral-bud explants”
t0“Arabidopsis thaliana”
Claim · model-written summary
Somatic embryos became normally formed plantlets.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- young plantlets with normal morphologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The somatic embryos grew into young plantlets with normal morphology, including cotyledons, hypocotyls, and roots”
t0“Arabidopsis thaliana”
Claim · model-written summary
ABI3 was expressed in the somatic embryos.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ABI3e0
- Outcome
- were expressed in these embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some embryo-specific genes (ABI3 and FUS3) were expressed in these embryos”
t0“Arabidopsis thaliana”
Claim · model-written summary
FUS3 was expressed in the somatic embryos.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- FUS3e0
- Outcome
- were expressed in these embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some embryo-specific genes (ABI3 and FUS3) were expressed in these embryos”
t0“Arabidopsis thaliana”
Claim · model-written summary
Osmotic stress induced somatic embryogenesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- osmotice0
- Outcome
- somatic embryogenesise0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several stresses (osmotic, heavy metal ion, and dehydration stress) induced somatic embryogenesis”
t0“Arabidopsis thaliana”
Claim · model-written summary
Heavy metal ion stress induced somatic embryogenesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- heavy metal ione0
- Outcome
- somatic embryogenesise0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several stresses (osmotic, heavy metal ion, and dehydration stress) induced somatic embryogenesis”
t0“Arabidopsis thaliana”
Claim · model-written summary
Dehydration stress induced somatic embryogenesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- dehydration stresse0
- Outcome
- somatic embryogenesise0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several stresses (osmotic, heavy metal ion, and dehydration stress) induced somatic embryogenesis”
t0“Arabidopsis thaliana”
Claim · model-written summary
Optimal stress treatment differed among stressors.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- optimum stress treatment differede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimum stress treatment differed between different stressors”
t0“Arabidopsis thaliana”
Claim · model-written summary
A six to nine hour mannitol treatment was optimal for somatic embryogenesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- 6-9 h of culture on solid B5 medium containing 0.7 m mannitole0
- Outcome
- somatic embryogenesise0
- Direction
- optimale0
- Qualifier
- transferred to B5 medium containing 2,4-dichlorophenoxyacetic acid (2,4-D, 4.5 microm)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimal conditions for somatic embryogenesis were 6-9 h of culture on solid B5 medium containing 0.7 m mannitol, after which the explants were transferred to B5 medium containing 2,4-dichlorophenoxyacetic acid (2,4-D, 4.5 microm)”
t0“Arabidopsis thaliana”
Claim · model-written summary
The culture system induced somatic embryogenesis in Ws.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- Wse0
- Outcome
- somatic embryogenesise0
- Value
- three major ecotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using this tissue culture system, we induced somatic embryogenesis in three major ecotypes of Arabidopsis thaliana-Ws, Col, and Ler”
Claim · model-written summary
The culture system induced somatic embryogenesis in Col.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- Cole0
- Outcome
- somatic embryogenesise0
- Value
- three major ecotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using this tissue culture system, we induced somatic embryogenesis in three major ecotypes of Arabidopsis thaliana-Ws, Col, and Ler”
Claim · model-written summary
The culture system induced somatic embryogenesis in Ler.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- Lere0
- Outcome
- somatic embryogenesise0
- Value
- three major ecotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using this tissue culture system, we induced somatic embryogenesis in three major ecotypes of Arabidopsis thaliana-Ws, Col, and Ler”
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See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.