Abstract
The oligopeptide transporter (OPT) family contains nine members in Arabidopsis. While there is some evidence that AtOPTs mediate the uptake of tetra- and pentapeptides, OPT homologs in rice (Oryza sativa; OsGT1) and Indian mustard (Brassica juncea; BjGT1) have been described as transporters of glutathione derivatives. This study investigates the possibility that two members of the AtOPT family, AtOPT6 and AtOPT7, may also transport glutathione and its conjugates. Complementation of the hgt1met1 yeast double mutant by plant homologs of the yeast glutathione transporter HGT1 (AtOPT6, AtOPT7, OsGT1, BjGT1) did not restore the growth phenotype, unlike complementation by HGT1. By contrast, complementation by AtOPT6 restored growth of the hgt1 yeast mutant on a medium containing reduced (GSH) or oxidized glutathione as the sole sulfur source and induced uptake of [3H]GSH, whereas complementation by AtOPT7 did not. In these conditions, AtOPT6-dependent GSH uptake in yeast was mediated by a high affinity (Km = 400 microm) and a low affinity (Km = 5 mm) phase. It was strongly competed for by an excess oxidized glutathione and glutathione-N-ethylmaleimide conjugate. Growth assays of yeasts in the presence of cadmium (Cd) suggested that AtOPT6 may transport Cd and Cd/GSH conjugate. Reporter gene experiments showed that AtOPT6 is mainly expressed in dividing areas of the plant (cambium, areas of lateral root initiation). RNA blots on cell suspensions and real-time reverse transcription-PCR on Arabidopsis plants indicated that AtOPT6 expression is strongly induced by primisulfuron and, to a lesser extent, by abscisic acid but not by Cd. Altogether, the data show that the substrate specificity and the physiological functions of AtOPT members may be diverse. In addition to peptide transport, AtOPT6 is able to transport glutathione derivatives and metal complexes, and may be involved in stress resistance.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Plant homologs of the yeast glutathione transporter HGT1 (AtOPT6, AtOPT7, OsGT1, BjGT1) did not restore growth of the hgt1met1 yeast double mutant, unlike HGT1.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- plant homologs of the yeast glutathione transporter HGT1 (AtOPT6, AtOPT7, OsGT1, BjGT1)e1
- Gene
- AtOPT6, AtOPT7, OsGT1, BjGT1e1
- Outcome
- growth phenotypee1
- Direction
- did not restoree1
- Comparator
- complementation by HGT1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e1“Complementation of the hgt1met1 yeast double mutant by plant homologs of the yeast glutathione transporter HGT1 (AtOPT6, AtOPT7, OsGT1, BjGT1) did not restore the growth phenotype, unlike complementation by HGT1”
Claim · model-written summary
AtOPT6 complementation restored growth of the hgt1 yeast mutant on medium with reduced or oxidized glutathione as the sole sulfur source, whereas AtOPT7 did not.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- complementation by AtOPT6e1
- Gene
- AtOPT6e1
- Outcome
- growth of the hgt1 yeast mutante1
- Direction
- restorede1
- Qualifier
- on a medium containing reduced (GSH) or oxidized glutathione as the sole sulfur sourcee1
- Comparator
- complementation by AtOPT7e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e1“complementation by AtOPT6 restored growth of the hgt1 yeast mutant on a medium containing reduced (GSH) or oxidized glutathione as the sole sulfur source”
e2“whereas complementation by AtOPT7 did not”
Claim · model-written summary
AtOPT6 complementation induced uptake of [3H]GSH in yeast.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- complementation by AtOPT6e3
- Gene
- AtOPT6e3
- Outcome
- uptake of [3H]GSHe3
- Direction
- inducede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e3“complementation by AtOPT6 restored growth of the hgt1 yeast mutant on a medium containing reduced (GSH) or oxidized glutathione as the sole sulfur source and induced uptake of [3H]GSH”
Claim · model-written summary
AtOPT6-dependent GSH uptake in yeast had a high affinity phase with Km = 400 microm.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtOPT6e4
- Outcome
- AtOPT6-dependent GSH uptake in yeaste4
- Value
- Km = 400 microme4
- Qualifier
- a high affinitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e4“AtOPT6-dependent GSH uptake in yeast was mediated by a high affinity (Km = 400 microm) and a low affinity (Km = 5 mm) phase”
Claim · model-written summary
AtOPT6-dependent GSH uptake in yeast had a low affinity phase with Km = 5 mm.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtOPT6e4
- Outcome
- AtOPT6-dependent GSH uptake in yeaste4
- Value
- Km = 5 mme4
- Qualifier
- a low affinitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e4“AtOPT6-dependent GSH uptake in yeast was mediated by a high affinity (Km = 400 microm) and a low affinity (Km = 5 mm) phase”
Claim · model-written summary
Excess oxidized glutathione and glutathione-N-ethylmaleimide conjugate strongly competed for AtOPT6-dependent GSH uptake in yeast.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- excess oxidized glutathione and glutathione-N-ethylmaleimide conjugatee6
- Outcome
- AtOPT6-dependent GSH uptakee4
- Direction
- decreaseinferrede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e4“AtOPT6-dependent GSH uptake in yeast was mediated by a high affinity (Km = 400 microm) and a low affinity (Km = 5 mm) phase”
e6“It was strongly competed for by an excess oxidized glutathione and glutathione-N-ethylmaleimide conjugate”
Claim · model-written summary
Growth assays in cadmium suggested that AtOPT6 may transport Cd and Cd/GSH conjugate.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- cadmium (Cd)e7
- Gene
- AtOPT6e7
- Compound
- Cd/GSH conjugatee7
- Outcome
- Growth assays of yeastse7
- Qualifier
- may transport Cd and Cd/GSH conjugatee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e7“Growth assays of yeasts in the presence of cadmium (Cd) suggested that AtOPT6 may transport Cd and Cd/GSH conjugate”
Claim · model-written summary
AtOPT6 is mainly expressed in dividing areas of the plant, including the cambium and areas of lateral root initiation.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- dividing areas of the plant (cambium, areas of lateral root initiation)e1
- Gene
- AtOPT6e1
- Outcome
- mainly expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“real-time reverse transcription-PCR on Arabidopsis plants”
e1“Reporter gene experiments showed that AtOPT6 is mainly expressed in dividing areas of the plant (cambium, areas of lateral root initiation)”
Claim · model-written summary
Primisulfuron strongly induced AtOPT6 expression in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- primisulfurone1
- Gene
- AtOPT6e1
- Outcome
- AtOPT6 expressione1
- Direction
- strongly inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“real-time reverse transcription-PCR on Arabidopsis plants”
e1“indicated that AtOPT6 expression is strongly induced by primisulfuron and, to a lesser extent, by abscisic acid but not by Cd”
Claim · model-written summary
Abscisic acid induced AtOPT6 expression, to a lesser extent than primisulfuron.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- abscisic acide1
- Gene
- AtOPT6e1
- Outcome
- AtOPT6 expressione1
- Direction
- inducede1
- Qualifier
- to a lesser extente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“real-time reverse transcription-PCR on Arabidopsis plants”
e1“indicated that AtOPT6 expression is strongly induced by primisulfuron and, to a lesser extent, by abscisic acid but not by Cd”
Claim · model-written summary
Cadmium did not induce AtOPT6 expression.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Cde1
- Gene
- AtOPT6e1
- Outcome
- AtOPT6 expressione1
- Direction
- no_changeinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“real-time reverse transcription-PCR on Arabidopsis plants”
e1“indicated that AtOPT6 expression is strongly induced by primisulfuron and, to a lesser extent, by abscisic acid but not by Cd”
Claim · model-written summary
AtOPT6 may be involved in stress resistance.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtOPT6e1
- Outcome
- stress resistancee1
- Qualifier
- may be involvede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contains nine members in Arabidopsis”
e1“AtOPT6 is able to transport glutathione derivatives and metal complexes, and may be involved in stress resistance”
The same questions in other papers
cadmium atom · molecular: expression · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.