Abstract
Degradation of ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco, EC.4.1.1.39), due to elevation in atmospheric temperature, and extent of enzyme damage, due to varietal differences, were studied by [ S]‐methionine pulse‐chase, transcript level of the large subunit (LSU), and protease activity in two rice cultivars. The cultivars N 22 and IR 8 are certified as thermotolerant and thermosensitive, respectively. Differential responses were observed in both cultivars, with the N 22 showing greater thermostability of the Rubisco protein up to 45°C, whereas IR 8 was thermolabile. Elevation of the temperature to 50°C favored the degradation of the protein in both cultivars. The transcript level of the LSU, as studied by Northern blot hybridization, also showed thermostability in N 22, whereas it was thermosensitive in IR 8. Protease activity, as measured by Western blot analysis using purified Rubisco, revealed the same trend in both cultivars and was correlated with protein turnover by [ S]‐methionine and Northern blot analysis. The results indicate that genetic differences exist in two rice cultivars and that the heat‐tolerant cultivar has a protective mechanism against thermal degradation of Rubisco.
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Claims 6
Claim · model-written summary
N 22 showed greater Rubisco thermostability up to 45°C than thermolabile IR 8.
- Taxon
- rice
Oryza sativat0 - Material
- N 22e0
- Gene
- Rubiscoe0
- Outcome
- thermostability of the Rubisco proteine0
- Direction
- greatere0
- Value
- 45°Ce0
- Comparator
- IR 8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N 22 showing greater thermostability of the Rubisco protein up to 45°C, whereas IR 8 was thermolabile.”
t0“rice”
Claim · model-written summary
At 50°C Rubisco degradation was favored in both cultivars.
- Taxon
- riceOryza sativat0
- Treatment
- Elevation of the temperature to 50°Ce0
- Outcome
- degradation of the proteine0
- Direction
- favorede0
- Value
- 50°Ce0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevation of the temperature to 50°C favored the degradation of the protein in both cultivars.”
t0“rice”
Claim · model-written summary
LSU transcript levels were thermostable in N 22.
- Taxon
- riceOryza sativat0
- Material
- N 22e0
- Gene
- LSUe0
- Outcome
- transcript level of the LSUe0
- Direction
- thermostabilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of the LSU, as studied by Northern blot hybridization, also showed thermostability in N 22”
t0“rice”
Claim · model-written summary
LSU transcript levels were thermosensitive in IR 8.
- Taxon
- riceOryza sativat0
- Material
- IR 8e0
- Gene
- LSUe0
- Outcome
- thermosensitivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript level of the LSU, as studied by Northern blot hybridization, also showed thermostability in N 22, whereas it was thermosensitive in IR 8.”
t0“rice”
Claim · model-written summary
Protease activity showed the same cultivar trend and correlated with protein turnover.
- Taxon
- riceOryza sativat0
- Material
- both cultivarse0
- Gene
- Rubiscoe0
- Outcome
- Protease activitye0
- Qualifier
- protein turnovere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protease activity, as measured by Western blot analysis using purified Rubisco, revealed the same trend in both cultivars and was correlated with protein turnover”
t0“rice”
Claim · model-written summary
Authors infer a protective mechanism against Rubisco thermal degradation in the heat-tolerant cultivar.
- Taxon
- riceOryza sativat0
- Material
- heat‐tolerant cultivare0
- Gene
- Rubiscoe0
- Outcome
- protective mechanism against thermal degradation of Rubiscoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heat‐tolerant cultivar has a protective mechanism against thermal degradation of Rubisco.”
t0“rice”
The same questions in other papers
altitude · quality: protein · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.