Abstract
Abscisic acid (ABA) regulates the plant's adaptive responses to abiotic stresses. Over-expression of the 9-cis-epoxycarotenoid dioxygenase gene (SgNCED1) in the transgenic tobaccos increased ABA content and tolerance to drought and salt stresses. H2O2 and nitric oxide (NO) contents were enhanced in guard cells and mesophyll cells of the transgenic plants, accompanied with increased transcripts and activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). The enhancements of H2O2 and NO and transcripts and activities of antioxidant enzymes in the transgenic plants were blocked by pre-treatments with inhibitor of ABA biosynthesis, scavengers of H2O2 and NO, and inhibitors of NADPH oxidase and NO synthase-like (NOS-like). The elevated production of NO in the transgenic plants was blocked by scavenger of H2O2 and inhibitors of NADPH oxidase, whereas H2O2 level was not affected by scavenger of NO and inhibitor of NOS-like, indicating that H2O2 is essential for the elevated production of NO. The results demonstrate that the increased drought and salt tolerance in the transgenic plants is associated with ABA-induced production of H2O2 via NADPH oxidase and NO via NOS-like, which sequentially induce transcripts and activities of SOD, CAT, APX and GR.
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Claims 9
Claim · model-written summary
SgNCED1 overexpression increased tobacco ABA content and drought and salt tolerance.
- Taxon
- tobaccos
Nicotiana tabacume0 - Treatment
- Over-expression of the 9-cis-epoxycarotenoid dioxygenase gene (SgNCED1)e0
- Gene
- SgNCED1e0
- Outcome
- ABA content and tolerance to drought and salt stressese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of the 9-cis-epoxycarotenoid dioxygenase gene (SgNCED1) in the transgenic tobaccos increased ABA content and tolerance to drought and salt stresses.”
Claim · model-written summary
Transgenic tobacco had enhanced H2O2 and NO contents in guard and mesophyll cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- guard cells and mesophyll cellse0
- Outcome
- H2O2 and nitric oxide (NO) contentse0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“H2O2 and nitric oxide (NO) contents were enhanced in guard cells and mesophyll cells of the transgenic plants”
Claim · model-written summary
Transgenic tobacco had increased antioxidant enzyme transcripts and activities.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR)e0
- Outcome
- transcripts and activities of antioxidant enzymese0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“increased transcripts and activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR)”
Claim · model-written summary
ABA biosynthesis inhibition and ROS and NO scavenging or production inhibition blocked enhanced H2O2 and NO in transgenic tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- pre-treatments with inhibitor of ABA biosynthesis, scavengers of H2O2 and NO, and inhibitors of NADPH oxidase and NO synthase-like (NOS-like)e0
- Outcome
- enhancements of H2O2 and NOe0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“The enhancements of H2O2 and NO and transcripts and activities of antioxidant enzymes in the transgenic plants were blocked by pre-treatments with inhibitor of ABA biosynthesis, scavengers of H2O2 and NO, and inhibitors of NADPH oxidase and NO synthase-like (NOS-like).”
Claim · model-written summary
The same pretreatments blocked enhanced antioxidant enzyme transcripts and activities.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- pre-treatments with inhibitor of ABA biosynthesis, scavengers of H2O2 and NO, and inhibitors of NADPH oxidase and NO synthase-like (NOS-like)e0
- Outcome
- transcripts and activities of antioxidant enzymese0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“The enhancements of H2O2 and NO and transcripts and activities of antioxidant enzymes in the transgenic plants were blocked by pre-treatments with inhibitor of ABA biosynthesis, scavengers of H2O2 and NO, and inhibitors of NADPH oxidase and NO synthase-like (NOS-like).”
Claim · model-written summary
H2O2 scavenging and NADPH oxidase inhibition blocked elevated NO in transgenic tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- scavenger of H2O2 and inhibitors of NADPH oxidasee0
- Outcome
- elevated production of NOe0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“The elevated production of NO in the transgenic plants was blocked by scavenger of H2O2 and inhibitors of NADPH oxidase”
Claim · model-written summary
NO scavenging and NOS-like inhibition did not affect H2O2 in transgenic tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- scavenger of NO and inhibitor of NOS-likee0
- Outcome
- H2O2 levele0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“H2O2 level was not affected by scavenger of NO and inhibitor of NOS-like”
Claim · model-written summary
The authors infer that H2O2 is essential to elevated NO production.
- Taxon
- tobaccoNicotiana tabacumt0
- Compound
- H2O2e0
- Outcome
- elevated production of NOe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“H2O2 is essential for the elevated production of NO”
Claim · model-written summary
The authors associate increased tobacco drought and salt tolerance with an ABA-H2O2-NO-antioxidant signaling sequence.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- increased drought and salt tolerancee0
- Qualifier
- ABA-induced production of H2O2 via NADPH oxidase and NO via NOS-likee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tobacco”
e0“increased drought and salt tolerance in the transgenic plants is associated with ABA-induced production of H2O2 via NADPH oxidase and NO via NOS-like, which sequentially induce transcripts and activities of SOD, CAT, APX and GR”
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