Abstract
UV RESISTANCE LOCUS8 (UVR8) signaling involves CONSTITUTIVELY PHOTOMORPHOGENIC1, the ELONGATED HYPOCOTYL5 (HY5) transcription factor, and the closely related HY5 HOMOLOG. Some UV-B responses mediated by UVR8 are also regulated by nitric oxide (NO), a bioactive molecule that orchestrates a wide range of processes in plants. In this study, we investigated the participation of the UVR8 pathway and its interaction with NO in UV-B-induced stomatal movements in Arabidopsis (Arabidopsis thaliana). Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsberg erecta closed in response to increasing UV-B fluence rates, with maximal closure after 3-h exposure to 5.46 μmol m⁻² s⁻¹ UV-B. Both hydrogen peroxide (H₂O₂) and NO increased in response to UV-B, and stomatal closure was maintained by NO up to 24 h after the beginning of exposure. Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, did not close in response to UV-B, although H₂O₂ still increased. When the uvr8-1 null mutant was exposed to UV-B, stomata remained open, irrespective of the fluence rate. Neither NO nor H₂O₂ increased in stomata of the uvr8-1 mutant. However, the NO donor S-nitrosoglutathione induced closure of uvr8-1 stomata to the same extent as in the wild type. Experiments with mutants in UVR8 signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-induced stomatal closure. This research provides evidence that the UVR8 pathway regulates stomatal closure by a mechanism involving both H₂O₂ and NO generation in response to UV-B exposure.
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Claims 10
Claim · model-written summary
UV-B exposure closed Arabidopsis stomata with maximal closure after three hours at 5.46 μmol m⁻² s⁻¹.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- UV-Be0
- Outcome
- Stomatae0
- Direction
- closede0
- Value
- 5.46 μmol m⁻² s⁻¹e0
- Qualifier
- 3-h exposuree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomata in abaxial epidermal strips of Arabidopsis ecotype Landsberg erecta closed in response to increasing UV-B fluence rates, with maximal closure after 3-h exposure to 5.46 μmol m⁻² s⁻¹ UV-B”
Claim · model-written summary
Hydrogen peroxide increased in response to UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-Be0
- Outcome
- hydrogen peroxide (H₂O₂)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both hydrogen peroxide (H₂O₂) and NO increased in response to UV-B”
t0“Arabidopsis”
Claim · model-written summary
Nitric oxide increased in response to UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-Be0
- Outcome
- NOe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both hydrogen peroxide (H₂O₂) and NO increased in response to UV-B”
t0“Arabidopsis”
Claim · model-written summary
Nitric oxide maintained stomatal closure up to 24 hours.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- NOe0
- Outcome
- stomatal closuree0
- Qualifier
- up to 24 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stomatal closure was maintained by NO up to 24 h after the beginning of exposure”
t0“Arabidopsis”
Claim · model-written summary
NO dioxygenase plants did not close stomata under UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-Be0
- Gene
- bacterial NO dioxygenasee0
- Outcome
- Stomatae0
- Direction
- did not closee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, did not close in response to UV-B”
t0“Arabidopsis”
Claim · model-written summary
Hydrogen peroxide still increased in NO dioxygenase plants under UV-B.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- UV-Be0
- Gene
- bacterial NO dioxygenasee0
- Outcome
- H₂O₂e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomata of plants expressing bacterial NO dioxygenase, which prevents NO accumulation, did not close in response to UV-B, although H₂O₂ still increased”
t0“Arabidopsis”
Claim · model-written summary
UV-B exposed uvr8-1 stomata remained open at all fluence rates.
- Taxon
- ArabidopsisArabidopsist0
- Material
- uvr8-1 null mutante0
- Treatment
- UV-Be0
- Outcome
- stomatae0
- Direction
- remained opene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the uvr8-1 null mutant was exposed to UV-B, stomata remained open, irrespective of the fluence rate”
t0“Arabidopsis”
Claim · model-written summary
NO and H₂O₂ did not increase in uvr8-1 stomata.
- Taxon
- ArabidopsisArabidopsist0
- Material
- uvr8-1 mutante0
- Outcome
- NO nor H₂O₂e0
- Direction
- Neithere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither NO nor H₂O₂ increased in stomata of the uvr8-1 mutant”
t0“Arabidopsis”
Claim · model-written summary
S-nitrosoglutathione closed uvr8-1 stomata to the wild-type extent.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- S-nitrosoglutathionee0
- Outcome
- uvr8-1 stomatae0
- Direction
- induced closuree0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NO donor S-nitrosoglutathione induced closure of uvr8-1 stomata to the same extent as in the wild type”
t0“Arabidopsis”
Claim · model-written summary
COP1, HY5 and HY5 HOMOLOG were implicated in UV-B stomatal closure.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOGe0
- Outcome
- UV-B-induced stomatal closuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experiments with mutants in UVR8 signaling components implicated CONSTITUTIVELY PHOTOMORPHOGENIC1, HY5, and HY5 HOMOLOG in UV-B-induced stomatal closure”
t0“Arabidopsis”
The same questions in other papers
uv · physiology: gas exchange · ArabidopsisPositive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.