Abstract
Wcor15, a member of the wheat cold-responsive (Cor) gene family, has been isolated and characterized. The deduced polypeptide WCOR15 (MW=14.7 kDa) showed high homology to the previously identified wheat and barley COR proteins. Southern blot analysis using diploid, tetraploid and hexaploid wheat and diploid Aegilops species showed that the wheat and related wild genomes possessed multiple copies of Wcor15 homologues. Five copies were assigned to the homologous group 2 chromosomes by nulli-tetrasomic analysis. Northern blot analysis showed that expression of Wcor15 was specifically induced by low-temperature. Homologous transcripts accumulated in leaves, and light markedly increased their steady-state levels. Bombardment-mediated transient expression analysis of a chimeric CaMV 35S::Wcor15-GFP construct showed protein-targeting to epidermal guard cell chloroplasts in excised spiderwort leaves. A promoter of Wcor15 contained at least three CRT/DRE-like sequence motifs found in Arabidopsis Cor genes and induced the reporter GUS gene expression in leaves of transgenic tobacco plants under low-temperature and light conditions. These results suggest that the functional Cor gene system involving the CRT/DRE cis-element is conserved in both monocotyledonous and dicotyledonous plants.
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Claims 10
Claim · model-written summary
Wcor15 was isolated and characterized.
- Taxon
- wheat
Triticum aestivume0 - Gene
- Wcor15e0
- Outcome
- Wcor15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wcor15, a member of the wheat cold-responsive (Cor) gene family, has been isolated and characterized.”
Claim · model-written summary
WCOR15 polypeptide showed high homology to wheat and barley COR proteins.
- Taxon
- wheatTriticum aestivume0
- Gene
- WCOR15e0
- Outcome
- high homologye0
- Value
- 14.7 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced polypeptide WCOR15 (MW=14.7 kDa) showed high homology to the previously identified wheat and barley COR proteins.”
Claim · model-written summary
Wheat and Aegilops genomes contained multiple Wcor15 homologues.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Wcor15e0
- Outcome
- multiple copies of Wcor15 homologuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wheat and related wild genomes possessed multiple copies of Wcor15 homologues.”
t0“wheat (Triticum aestivum L.)”
Claim · model-written summary
Five copies mapped to homologous group 2 chromosomes.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- copiese0
- Value
- Five copiese0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e0“Five copies were assigned to the homologous group 2 chromosomes”
Claim · model-written summary
Low temperature specifically induced Wcor15 expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- low-temperaturee0
- Gene
- Wcor15e0
- Outcome
- expression of Wcor15e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e0“expression of Wcor15 was specifically induced by low-temperature”
Claim · model-written summary
Homologous transcripts accumulated in leaves.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- leavese0
- Outcome
- Homologous transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e0“Homologous transcripts accumulated in leaves”
Claim · model-written summary
Light markedly increased transcript levels.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- lighte0
- Outcome
- steady-state levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e0“light markedly increased their steady-state levels”
Claim · model-written summary
Wcor15 GFP protein targeted guard cell chloroplasts in spiderwort leaves.
- Taxon
- spiderwort
Tradescantia fluminensise0 - Organ / stage
- leavese0
- Gene
- Wcor15-GFPe0
- Outcome
- protein-targeting to epidermal guard cell chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wcor15-GFP construct showed protein-targeting to epidermal guard cell chloroplasts in excised spiderwort leaves”
Claim · model-written summary
Wcor15 promoter contained at least three CRT/DRE-like motifs.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Wcor15e0
- Outcome
- CRT/DRE-like sequence motifse0
- Value
- at least threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e0“A promoter of Wcor15 contained at least three CRT/DRE-like sequence motifs”
Claim · model-written summary
The Wcor15 promoter induced GUS expression in transgenic tobacco leaves under low temperature and light.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- low-temperature and light conditionse0
- Gene
- GUSe0
- Outcome
- GUS gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“induced the reporter GUS gene expression in leaves of transgenic tobacco plants under low-temperature and light conditions.”
The same questions in other papers
cold · molecular: expression · Triticum aestivum (wheat)Results differ
33 papers in the same direction as this paper · 15 in the opposite direction
- positive
- positive
- positive
45 papers more in this group
See all 49 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.