Paper · 2011 · International Journal of Agricultural Technology

Biocontrol activity of Trichoderma viride and Pseudomonas fluorescens against Phytophthora infestans under greenhouse conditions

E. D. Zegeye; Amutha Santhanam; D. Gorfu; et al.

7
claims
15
verbatim quotes
43
citations
2
research areas
—
Not open access

Abstract

The biocontrol potential of Trichoderma viride-ES1 and Pseudomonas fluorescens-Bak150 against potato late blight pathogen, Phytophthora infestans, were studied in vitro and under greenhouse conditions. In vitro antagonism test carried out between T. viride and P. infestans showed a radial growth inhibition of the pathogen by 36.7% and a complete overgrowth of T. viride on P. infestans later, whereas P. fluorescens inhibited the radial growth of the pathogen by 88%. Foliar spray method used in the greenhouse trials involved foliar spray of healthy potato plants with suspensions of (i) T. viride only, (ii) P. fluorescens only, (iii) mixed culture of T. viride and P. fluorescens, and (iv) Mancozeb, followed by spraying the pathogen inoculum three days later. The infected leaf area was measured weekly and area under the disease progress curve (AUDPC) was calculated and compared among the treatments. The result showed that T. viride (AUDPC=260) and P. fluorescens (AUDPC=765.1) significantly (P<0.05) reduced the disease compared to the untreated check (AUDPC=1045). T. viride was found to be more efficient than P. fluorescens and mixed culture. No significant difference was observed between the mixed culture and the inoculated/untreated check. This study revealed that the foliar application of T. viride-ES1 has good potential in controlling the late blight disease of potato.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In vitro, T. viride inhibited radial growth of Phytophthora infestans by 36.7%.

Treatment
T. viridee1
Interactor
Phytophthora infestansPhytophthora infestanse0pathogen
Second interactor
T. virideTrichoderma viridee1biocontrol_agent
Outcome
radial growth inhibition of the pathogene1
Direction
inhibitione1
Value
36.7%e1
Setting
In vitroe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗
Phytophthora infestans
Phytophthora infestans · InteractorHoward F. Schwartz, Colorado State University, United States · CC BY 3.0 us · Wikimedia Commons ↗
Trichoderma viride
Trichoderma viride · Second interactorKatja Schulz · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The biocontrol potential of Trichoderma viride-ES1 and Pseudomonas fluorescens-Bak150 against potato late blight pathogen, Phytophthora infestans”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
e1

“In vitro antagonism test carried out between T. viride and P. infestans showed a radial growth inhibition of the pathogen by 36.7%”

✓ located verbatim in the source · abstract, characters 202–332 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In vitro, P. fluorescens inhibited radial growth of Phytophthora infestans by 88%.

Treatment
P. fluorescense1
Interactor
Phytophthora infestansPhytophthora infestanse0pathogen
Second interactor
P. fluorescensPseudomonas fluorescense1biocontrol_agent
Outcome
radial growth of the pathogene1
Direction
inhibitede1
Value
88%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗
Phytophthora infestans
Phytophthora infestans · InteractorHoward F. Schwartz, Colorado State University, United States · CC BY 3.0 us · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · Second interactorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The biocontrol potential of Trichoderma viride-ES1 and Pseudomonas fluorescens-Bak150 against potato late blight pathogen, Phytophthora infestans”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
e1

“whereas P. fluorescens inhibited the radial growth of the pathogen by 88%”

✓ located verbatim in the source · abstract, characters 395–468 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

In the greenhouse, foliar T. viride significantly reduced late blight (AUDPC 260) compared with the untreated check (1045).

Treatment
T. viridee1
Interactor
Phytophthora infestansPhytophthora infestanse0pathogen
Second interactor
T. virideTrichoderma viridee1biocontrol_agent
Outcome
AUDPCe1
Direction
reducede1
Value
AUDPC=260e1
Comparator
untreated checke1
Setting
greenhouse conditionst0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗
Phytophthora infestans
Phytophthora infestans · InteractorHoward F. Schwartz, Colorado State University, United States · CC BY 3.0 us · Wikimedia Commons ↗
Trichoderma viride
Trichoderma viride · Second interactorKatja Schulz · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The biocontrol potential of Trichoderma viride-ES1 and Pseudomonas fluorescens-Bak150 against potato late blight pathogen, Phytophthora infestans”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
t0

“under greenhouse conditions”

✓ located verbatim in the source · title, characters 101–128 · show in text ↑
e1

“T. viride (AUDPC=260) and P. fluorescens (AUDPC=765.1) significantly (P<0.05) reduced the disease compared to the untreated check (AUDPC=1045)”

✓ located verbatim in the source · abstract, characters 932–1074 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

In the greenhouse, foliar P. fluorescens significantly reduced late blight (AUDPC 765.1) compared with the untreated check (1045).

Treatment
P. fluorescense1
Interactor
Phytophthora infestansPhytophthora infestanse0pathogen
Second interactor
P. fluorescensPseudomonas fluorescense1biocontrol_agent
Outcome
AUDPCe1
Direction
reducede1
Value
AUDPC=765.1e1
Comparator
untreated checke1
Setting
greenhouse conditionst0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗
Phytophthora infestans
Phytophthora infestans · InteractorHoward F. Schwartz, Colorado State University, United States · CC BY 3.0 us · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · Second interactorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The biocontrol potential of Trichoderma viride-ES1 and Pseudomonas fluorescens-Bak150 against potato late blight pathogen, Phytophthora infestans”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
t0

“under greenhouse conditions”

✓ located verbatim in the source · title, characters 101–128 · show in text ↑
e1

“T. viride (AUDPC=260) and P. fluorescens (AUDPC=765.1) significantly (P<0.05) reduced the disease compared to the untreated check (AUDPC=1045)”

✓ located verbatim in the source · abstract, characters 932–1074 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

T. viride was more efficient than P. fluorescens and the mixed culture in controlling late blight.

Treatment
T. viridee1
Outcome
more efficiente1
Direction
more efficiente1
Comparator
P. fluorescens and mixed culturee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The biocontrol potential of Trichoderma viride-ES1 and Pseudomonas fluorescens-Bak150 against potato late blight pathogen, Phytophthora infestans”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
e1

“T. viride was found to be more efficient than P. fluorescens and mixed culture”

✓ located verbatim in the source · abstract, characters 1076–1154 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The mixed culture did not differ significantly from the inoculated, untreated check.

Treatment
mixed culturee1
Outcome
No significant differencee1
Direction
No significant differencee1
Comparator
inoculated/untreated checke1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The biocontrol potential of Trichoderma viride-ES1 and Pseudomonas fluorescens-Bak150 against potato late blight pathogen, Phytophthora infestans”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
e1

“No significant difference was observed between the mixed culture and the inoculated/untreated check”

✓ located verbatim in the source · abstract, characters 1156–1255 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Foliar application of T. viride-ES1 has good potential for controlling potato late blight.

Outcome
good potential in controlling the late blight diseasee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the foliar application of T. viride-ES1 has good potential in controlling the late blight disease of potato”

✓ located verbatim in the source · abstract, characters 1282–1389 · show in text ↑

Pseudomonas fluorescens · Phytophthora infestans · Solanum tuberosum (potato)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Trichoderma viride · Phytophthora infestans · Solanum tuberosum (potato)Results agree

8 papers: 8 positive, 0 negative or no effect

7 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.