Abstract
BACKGROUND: Soybean cyst nematode (Heterodera glycines, SCN) is the most economically damaging pathogen of soybean (Glycine max) in the U.S. The Rhg1 locus is repeatedly observed as the quantitative trait locus with the greatest impact on SCN resistance. The Glyma18g02680.1 gene at the Rhg1 locus that encodes an apparent leucine-rich repeat transmembrane receptor-kinase (LRR-kinase) has been proposed to be the SCN resistance gene, but its function has not been confirmed. Generation of fertile transgenic soybean lines is difficult but methods have been published that test SCN resistance in transgenic roots generated with Agrobacterium rhizogenes. RESULTS: We report use of artificial microRNA (amiRNA) for gene silencing in soybean, refinements to transgenic root SCN resistance assays, and functional tests of the Rhg1 locus LRR-kinase gene. A nematode demographics assay monitored infecting nematode populations for their progress through developmental stages two weeks after inoculation, as a metric for SCN resistance. Significant differences were observed between resistant and susceptible control genotypes. Introduction of the Rhg1 locus LRR-kinase gene (genomic promoter/coding region/terminator; Peking/PI 437654-derived SCN-resistant source), into rhg1- SCN-susceptible plant lines carrying the resistant-source Rhg4+ locus, provided no significant increases in SCN resistance. Use of amiRNA to reduce expression of the LRR-kinase gene from the Rhg1 locus of Fayette (PI 88788 source of Rhg1) also did not detectably alter resistance to SCN. However, silencing of the LRR-kinase gene did have impacts on root development. CONCLUSION: The nematode demographics assay can expedite testing of transgenic roots for SCN resistance. amiRNAs and the pSM103 vector that drives interchangeable amiRNA constructs through a soybean polyubiqutin promoter (Gmubi), with an intron-GFP marker for detection of transgenic roots, may have widespread use in legume biology. Studies in which expression of the Rhg1 locus LRR-kinase gene from different resistance sources was either reduced or complemented did not reveal significant impacts on SCN resistance.
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Claims 4
Claim · model-written summary
The nematode demographics assay in transgenic roots found significant differences between resistant and susceptible control genotypes of soybean.
- Taxon
- Glycine max
Glycine maxe0 - Interactor
- Heterodera glycines
Heterodera glycinese0pathogen - Outcome
- significant differencese2
- Comparator
- resistant and susceptible control genotypese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean cyst nematode (Heterodera glycines, SCN) is the most economically damaging pathogen of soybean (Glycine max) in the U.S.”
e1“A nematode demographics assay monitored infecting nematode populations for their progress through developmental stages two weeks after inoculation, as a metric for SCN resistance”
e2“Significant differences were observed between resistant and susceptible control genotypes”
Claim · model-written summary
Introducing the Rhg1 locus LRR-kinase gene into rhg1- SCN-susceptible soybean lines gave no significant increase in SCN resistance.
- Taxon
- Glycine maxGlycine maxe0
- Treatment
- Introduction of the Rhg1 locus LRR-kinase genee3
- Interactor
- SCNe3pathogen
- Gene
- LRR-kinase genee3
- Outcome
- SCN resistancee3
- Direction
- no significant increasese3
- Comparator
- rhg1- SCN-susceptible plant linese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean cyst nematode (Heterodera glycines, SCN) is the most economically damaging pathogen of soybean (Glycine max) in the U.S.”
e3“Introduction of the Rhg1 locus LRR-kinase gene (genomic promoter/coding region/terminator; Peking/PI 437654-derived SCN-resistant source), into rhg1- SCN-susceptible plant lines carrying the resistant-source Rhg4+ locus, provided no significant increases in SCN resistance”
Claim · model-written summary
Reducing LRR-kinase gene expression at the Rhg1 locus of Fayette by amiRNA did not detectably alter resistance to SCN.
- Taxon
- Glycine maxGlycine maxe0
- Material
- Fayettee4
- Treatment
- Use of amiRNA to reduce expression of the LRR-kinase genee4
- Interactor
- SCNe4pathogen
- Outcome
- resistance to SCNe4
- Direction
- did not detectably altere4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean cyst nematode (Heterodera glycines, SCN) is the most economically damaging pathogen of soybean (Glycine max) in the U.S.”
e4“Use of amiRNA to reduce expression of the LRR-kinase gene from the Rhg1 locus of Fayette (PI 88788 source of Rhg1) also did not detectably alter resistance to SCN”
Claim · model-written summary
Silencing of the LRR-kinase gene had impacts on soybean root development.
- Taxon
- Glycine maxGlycine maxe0
- Treatment
- silencing of the LRR-kinase genee5
- Outcome
- root developmente5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean cyst nematode (Heterodera glycines, SCN) is the most economically damaging pathogen of soybean (Glycine max) in the U.S.”
e5“However, silencing of the LRR-kinase gene did have impacts on root development”
The same questions in other papers
transgenic · disease · Glycine max (soybean)Positive and negative results
6 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
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