Abstract
Nitrogen (N), auxin and cytokinins (CTKs) play important roles in regulating the growth of rice tiller buds. However, little is known about the underlying mechanisms and the relationships between them in the regulation of tiller bud growth in rice. In the present study, N and auxin indole-3-acetic acid (IAA) were used to regulate tiller bud growth in Nanjing 44 rice, a japonica cultivar. The tiller buds grew rapidly after external application of 40 mg L -1 N; significantly elevated CTK levels were found in tiller buds and nodes preceding bud growth. However, external application of IAA inhibited the growth of tiller buds and reversed the increase in CTK levels in tiller buds and nodes induced by N. According to previous studies, the rice adenosine phosphate isopentenyltransferase (OsIPT ) genes encode a key enzyme in CTK biosynthesis. To determine the site of CTK synthesis involved in bud growth, we examined the expression patterns of the OsIPT genes in tiller buds, tiller nodes and roots. Our results indicate that the CTKs that promote tiller bud growth are biosynthesised mainly in the tiller nodes, and there is little or no contribution from the roots. N and IAA regulated the expression of the OsIPT genes and inhibited the biosynthesis of CTKs in the tiller nodes, thus regulating the growth of rice tiller buds.
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Claims 8
Claim · model-written summary
External 40 mg L -1 N caused rapid tiller bud growth.
- Taxon
- rice
Oryza sativat0 - Organ / stage
- tiller budse0
- Treatment
- external application of 40 mg L -1 Ne0
- Outcome
- tiller budse0
- Direction
- grew rapidlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tiller buds grew rapidly after external application of 40 mg L -1 N”
t0“rice”
Claim · model-written summary
N application significantly elevated CTK levels in tiller buds and nodes before bud growth.
- Taxon
- riceOryza sativat0
- Organ / stage
- tiller buds and nodese0
- Treatment
- Nt1
- Outcome
- CTK levelse0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significantly elevated CTK levels were found in tiller buds and nodes preceding bud growth”
t0“rice”
t1“The relationship between nitrogen, auxin and cytokinin in the growth regulation of rice (Oryza sativa L.) tiller buds”
Claim · model-written summary
IAA application inhibited tiller bud growth.
- Taxon
- riceOryza sativat0
- Treatment
- external application of IAAe0
- Outcome
- growth of tiller budse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“external application of IAA inhibited the growth of tiller buds”
t0“rice”
Claim · model-written summary
IAA reversed N-induced CTK elevation in buds and nodes.
- Taxon
- riceOryza sativat0
- Organ / stage
- tiller buds and nodese0
- Treatment
- IAAe1
- Outcome
- CTK levelse0
- Direction
- reversed the increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reversed the increase in CTK levels in tiller buds and nodes induced by N”
t0“rice”
t1“The relationship between nitrogen, auxin and cytokinin in the growth regulation of rice (Oryza sativa L.) tiller buds”
e1“external application of IAA inhibited the growth of tiller buds”
Claim · model-written summary
Growth-promoting CTKs were synthesized mainly in tiller nodes.
- Taxon
- riceOryza sativat0
- Organ / stage
- tiller nodese0
- Outcome
- CTKs that promote tiller bud growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the CTKs that promote tiller bud growth are biosynthesised mainly in the tiller nodes”
t0“rice”
Claim · model-written summary
Roots contributed little or nothing to growth-promoting CTKs.
- Taxon
- riceOryza sativat0
- Organ / stage
- rootse0
- Outcome
- contribution from the rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is little or no contribution from the roots”
t0“rice”
Claim · model-written summary
N and IAA regulated OsIPT gene expression.
- Taxon
- riceOryza sativat0
- Treatment
- N and IAAe0
- Gene
- OsIPT genese0
- Outcome
- expression of the OsIPT genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N and IAA regulated the expression of the OsIPT genes”
t0“rice”
Claim · model-written summary
N and IAA inhibited CTK biosynthesis in tiller nodes.
- Taxon
- riceOryza sativat0
- Organ / stage
- tiller nodese0
- Treatment
- N and IAAe0
- Outcome
- biosynthesis of CTKse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N and IAA regulated the expression of the OsIPT genes and inhibited the biosynthesis of CTKs in the tiller nodes”
t0“rice”
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indole-3-acetic acid · yield · Oryza sativa (rice)Results differ
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1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.