Abstract
Nitric oxide (NO) is a key redox-active, small molecule involved in various aspects of plant growth and development. Here, we report the identification of an NO accumulation mutant, nitric oxide excess1 (noe1), in rice (Oryza sativa), the isolation of the corresponding gene, and the analysis of its role in NO-mediated leaf cell death. Map-based cloning revealed that NOE1 encoded a rice catalase, OsCATC. Furthermore, noe1 resulted in an increase of hydrogen peroxide (H(2)O(2)) in the leaves, which consequently promoted NO production via the activation of nitrate reductase. The removal of excess NO reduced cell death in both leaves and suspension cultures derived from noe1 plants, implicating NO as an important endogenous mediator of H(2)O(2)-induced leaf cell death. Reduction of intracellular S-nitrosothiol (SNO) levels, generated by overexpression of rice S-nitrosoglutathione reductase gene (GSNOR1), which regulates global levels of protein S-nitrosylation, alleviated leaf cell death in noe1 plants. Thus, S-nitrosylation was also involved in light-dependent leaf cell death in noe1. Utilizing the biotin-switch assay, nanoliquid chromatography, and tandem mass spectrometry, S-nitrosylated proteins were identified in both wild-type and noe1 plants. NO targets identified only in noe1 plants included glyceraldehyde 3-phosphate dehydrogenase and thioredoxin, which have been reported to be involved in S-nitrosylation-regulated cell death in animals. Collectively, our data suggest that both NO and SNOs are important mediators in the process of H(2)O(2)-induced leaf cell death in rice.
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Claims 10
Claim · model-written summary
An NO accumulation mutant called noe1 was identified in rice.
- Taxon
- rice (Oryza sativa)
Oryza sativae0 - Material
- nitric oxide excess1 (noe1)e0
- Outcome
- NO accumulation mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identification of an NO accumulation mutant, nitric oxide excess1 (noe1), in rice (Oryza sativa)”
Claim · model-written summary
NOE1 encodes the rice catalase OsCATC.
- Taxon
- riceOryza sativae0
- Gene
- NOE1e0
- Outcome
- encoded a rice catalase, OsCATCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Map-based cloning revealed that NOE1 encoded a rice catalase, OsCATC”
Claim · model-written summary
The noe1 mutant increased hydrogen peroxide in leaves.
- Taxon
- RiceOryza sativat0
- Material
- noe1e0
- Outcome
- hydrogen peroxide (H(2)O(2)) in the leavese0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“noe1 resulted in an increase of hydrogen peroxide (H(2)O(2)) in the leaves”
t0“Rice”
Claim · model-written summary
Hydrogen peroxide accumulation promoted NO production through nitrate reductase activation.
- Taxon
- RiceOryza sativat0
- Gene
- nitrate reductasee0
- Compound
- hydrogen peroxide (H(2)O(2))e1
- Outcome
- NO productione0
- Direction
- promotede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which consequently promoted NO production via the activation of nitrate reductase”
e1“increase of hydrogen peroxide (H(2)O(2)) in the leaves”
t0“Rice”
Claim · model-written summary
Removing excess NO reduced leaf cell death in noe1 plants.
- Taxon
- RiceOryza sativat0
- Material
- noe1e0
- Treatment
- removal of excess NOe0
- Outcome
- cell death in both leaves and suspension culturese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The removal of excess NO reduced cell death in both leaves and suspension cultures derived from noe1 plants”
t0“Rice”
Claim · model-written summary
Overexpressing GSNOR1 lowered SNO levels and alleviated noe1 leaf cell death.
- Taxon
- riceOryza sativae0
- Material
- noe1e0
- Treatment
- overexpression of rice S-nitrosoglutathione reductase gene (GSNOR1)e0
- Gene
- rice S-nitrosoglutathione reductase gene (GSNOR1)e0
- Outcome
- leaf cell deathe0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduction of intracellular S-nitrosothiol (SNO) levels, generated by overexpression of rice S-nitrosoglutathione reductase gene (GSNOR1), which regulates global levels of protein S-nitrosylation, alleviated leaf cell death in noe1 plants”
Claim · model-written summary
S-nitrosylated proteins were identified in wild-type plants.
- Taxon
- RiceOryza sativat0
- Material
- wild-typee0
- Outcome
- S-nitrosylated proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S-nitrosylated proteins were identified in both wild-type and noe1 plants”
t0“Rice”
Claim · model-written summary
S-nitrosylated proteins were identified in noe1 plants.
- Taxon
- RiceOryza sativat0
- Material
- noe1e0
- Outcome
- S-nitrosylated proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S-nitrosylated proteins were identified in both wild-type and noe1 plants”
t0“Rice”
Claim · model-written summary
Glyceraldehyde 3-phosphate dehydrogenase and thioredoxin were NO targets unique to noe1 plants.
- Taxon
- RiceOryza sativat0
- Material
- noe1e0
- Gene
- glyceraldehyde 3-phosphate dehydrogenase and thioredoxine0
- Outcome
- NO targetse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO targets identified only in noe1 plants included glyceraldehyde 3-phosphate dehydrogenase and thioredoxin”
t0“Rice”
Claim · model-written summary
The authors infer that NO and SNOs mediate hydrogen peroxide induced leaf cell death.
- Taxon
- riceOryza sativae0
- Compound
- NO and SNOse0
- Outcome
- H(2)O(2)-induced leaf cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both NO and SNOs are important mediators in the process of H(2)O(2)-induced leaf cell death in rice”
The same questions in other papers
nitric oxide · cytology · Oryza sativa (rice)Positive and negative results
3 in the opposite direction
- negative
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.