Abstract
We generated transgenic tomato plants with altered expression of heat stress transcription factor HsfA1. Plants with 10-fold overexpression of HsfA1 (OE plants) were characterized by a single HsfA1 transgene cassette, whereas plants harboring a tandem inverted repeat of the cassette showed cosuppression (CS plants) by posttranscriptional silencing of the HsfA1 gene connected with formation of small interfering RNAs. Under normal growth conditions, major developmental parameters were similar for wild-type (WT), OE, and CS plants. However, CS plants and fruits were extremely sensitive to elevated temperatures, because heat stress-induced synthesis of chaperones and Hsfs was strongly reduced or lacking. Despite the complexity of the plant Hsf family with at least 17 members in tomato, HsfA1 has a unique function as master regulator for induced thermotolerance. Using transient reporter assays with mesophyll protoplasts from WT tomato, we demonstrated that plasmid-encoded HsfA1 and HsfA2 were well expressed. However, in CS protoplasts the cosuppression phenomenon was faithfully reproduced. Only transformation with HsfA2 expression plasmid led to normal expression of the transcription factor and reporter gene activation, whereas even high amounts of HsfA1 expression plasmids were silenced. Thermotolerance in CS protoplasts was restored by plasmid-borne HsfA2, resulting in expression of chaperones, thermoprotection of firefly luciferase, and assembly of heat stress granules.
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Claims 11
Claim · model-written summary
HsfA1 overexpressing plants contained one transgene cassette.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- OE plantse0
- Gene
- HsfA1e0
- Outcome
- a single HsfA1 transgene cassettee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants with 10-fold overexpression of HsfA1 (OE plants) were characterized by a single HsfA1 transgene cassette”
t0“tomato”
Claim · model-written summary
Tandem inverted cassette repeats led to HsfA1 cosuppression and small interfering RNAs.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- CS plantse0
- Gene
- HsfA1 genee0
- Outcome
- cosuppressione0
- Qualifier
- posttranscriptional silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants harboring a tandem inverted repeat of the cassette showed cosuppression (CS plants) by posttranscriptional silencing of the HsfA1 gene connected with formation of small interfering RNAs”
t0“tomato”
Claim · model-written summary
Developmental parameters were similar among wild type, overexpressing, and cosuppressed plants in normal growth.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- major developmental parameterse0
- Direction
- similare0
- Qualifier
- Under normal growth conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under normal growth conditions, major developmental parameters were similar for wild-type (WT), OE, and CS plants”
t0“tomato”
Claim · model-written summary
Cosuppressed tomato plants and fruits were highly temperature sensitive.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- CS plantse0
- Outcome
- sensitive to elevated temperaturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CS plants and fruits were extremely sensitive to elevated temperatures”
t0“tomato”
Claim · model-written summary
Heat stress induced chaperone and Hsf synthesis was reduced or absent in cosuppressed plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- Hsfse0
- Outcome
- synthesis of chaperones and Hsfse0
- Direction
- strongly reduced or lackinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heat stress-induced synthesis of chaperones and Hsfs was strongly reduced or lacking”
t0“tomato”
Claim · model-written summary
HsfA1 had a master regulator role in induced thermotolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- HsfA1e0
- Outcome
- induced thermotolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HsfA1 has a unique function as master regulator for induced thermotolerance”
t0“tomato”
Claim · model-written summary
Plasmid HsfA1 and HsfA2 were expressed in wild type protoplasts.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- mesophyll protoplastse1
- Gene
- HsfA1 and HsfA2e0
- Outcome
- well expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plasmid-encoded HsfA1 and HsfA2 were well expressed”
e1“mesophyll protoplasts from WT tomato”
Claim · model-written summary
Cosuppression was reproduced in CS protoplasts.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- CS protoplastse0
- Outcome
- cosuppression phenomenon was faithfully reproducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in CS protoplasts the cosuppression phenomenon was faithfully reproduced”
t0“tomato”
Claim · model-written summary
HsfA2 plasmid restored transcription factor expression and reporter activation in CS protoplasts.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- transformation with HsfA2 expression plasmide0
- Gene
- HsfA2e0
- Outcome
- normal expression of the transcription factor and reporter gene activatione0
- Direction
- normale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only transformation with HsfA2 expression plasmid led to normal expression of the transcription factor and reporter gene activation”
t0“tomato”
Claim · model-written summary
HsfA1 expression plasmids remained silenced at high amounts.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- HsfA1e0
- Outcome
- expression plasmids were silencede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“even high amounts of HsfA1 expression plasmids were silenced”
t0“tomato”
Claim · model-written summary
Plasmid borne HsfA2 restored thermotolerance, chaperones, luciferase thermoprotection, and heat stress granules in CS protoplasts.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- plasmid-borne HsfA2e0
- Gene
- HsfA2e0
- Outcome
- Thermotolerance in CS protoplastse0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thermotolerance in CS protoplasts was restored by plasmid-borne HsfA2, resulting in expression of chaperones, thermoprotection of firefly luciferase, and assembly of heat stress granules”
t0“tomato”
The same questions in other papers
transgenic · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.