Abstract
Tomato plants constitutively express a neutral leucine aminopeptidase (LAP-N) and an acidic LAP (LAP-A) during floral development and in leaves in response to insect infestation, wounding, and Pseudomonas syringae pv. tomato infection. To assess the physiological roles of LAP-A, a LapA-antisense construct (35S:asLapA1) was introduced into tomato. The 35S:asLapA1 plants had greatly reduced or showed undetectable levels of LAP-A and LAP-N proteins in healthy and wounded leaves and during floral development. Despite the loss of these aminopeptidases, no global changes in protein profiles were noted. The 35S:asLapA1 plants also exhibited no significant alteration in floral development and did not impact the growth and development of Manduca sexta and P. syringae pv. tomato growth rates during compatible or incompatible infections. To investigate the mechanism underlying the strong induction of LapA upon P. syringae pv. tomato infection, LapA expression was monitored after infection with coronatine-producing and -deficient P. syringae pv. tomato strains. LapA RNA and activity were detected only with the coronatine-producing P. syringae pv. tomato strain. Coronatine treatment of excised shoots caused increases in RNAs for jasmonic acid (JA)-regulated wound-response genes (LapA and pin2) but did not influence expression of a JA-regulated pathogenesis-related protein gene (PR-1). These results indicated that coronatine mimicked the wound response but was insufficient to activate JA-regulated PR genes.
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Claims 9
Claim · model-written summary
The 35S:asLapA1 construct greatly reduced or eliminated LAP-A and LAP-N proteins in tomato leaves and floral development.
- Taxon
- Tomato
Solanum lycopersicumt0 - Material
- 35S:asLapA1e0
- Organ / stage
- leavese0
- Treatment
- 35S:asLapA1e0
- Outcome
- LAP-A and LAP-N proteinse0
- Direction
- greatly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 35S:asLapA1 plants had greatly reduced or showed undetectable levels of LAP-A and LAP-N proteins in healthy and wounded leaves and during floral development”
t0“Tomato”
Claim · model-written summary
Loss of these aminopeptidases did not cause global changes in protein profiles.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- loss of these aminopeptidasese0
- Outcome
- protein profilese0
- Direction
- no global changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Despite the loss of these aminopeptidases, no global changes in protein profiles were noted”
t0“Tomato”
Claim · model-written summary
35S:asLapA1 plants showed no significant alteration in floral development.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- 35S:asLapA1e0
- Treatment
- 35S:asLapA1e0
- Outcome
- floral developmente0
- Direction
- no significant alteratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 35S:asLapA1 plants also exhibited no significant alteration in floral development”
t0“Tomato”
Claim · model-written summary
35S:asLapA1 plants did not affect Manduca sexta growth and development.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- 35S:asLapA1e0
- Interactor
- Manduca sexta
Manduca sextae0pest - Outcome
- growth and development of Manduca sextae0
- Direction
- did not impacte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 35S:asLapA1 plants also exhibited no significant alteration in floral development and did not impact the growth and development of Manduca sexta and P. syringae pv. tomato growth rates during compatible or incompatible infections”
t0“Tomato”
Claim · model-written summary
35S:asLapA1 plants did not affect P. syringae pv. tomato growth during infection.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- 35S:asLapA1e0
- Interactor
- P. syringae pv. tomato
Pseudomonas syringae pv. tomatoe0pathogen - Outcome
- P. syringae pv. tomato growth ratese0
- Direction
- did not impacte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 35S:asLapA1 plants also exhibited no significant alteration in floral development and did not impact the growth and development of Manduca sexta and P. syringae pv. tomato growth rates during compatible or incompatible infections”
t0“Tomato”
Claim · model-written summary
LapA RNA and activity were detected only after infection with the coronatine-producing P. syringae pv. tomato strain.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- coronatine-producing P. syringae pv. tomato straine0
- Interactor
- P. syringae pv. tomatoPseudomonas syringae pv. tomatoe0pathogen
- Gene
- LapAe0
- Outcome
- LapA RNA and activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LapA RNA and activity were detected only with the coronatine-producing P. syringae pv. tomato strain”
t0“Tomato”
Claim · model-written summary
Coronatine increased LapA and pin2 wound-response RNAs in excised tomato shoots.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- excised shootse0
- Treatment
- Coronatine treatmente0
- Gene
- LapA and pin2e0
- Outcome
- RNAs for jasmonic acid (JA)-regulated wound-response genese0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coronatine treatment of excised shoots caused increases in RNAs for jasmonic acid (JA)-regulated wound-response genes (LapA and pin2) but did not influence expression of a JA-regulated pathogenesis-related protein gene (PR-1)”
t0“Tomato”
Claim · model-written summary
Coronatine did not influence PR-1 expression in excised tomato shoots.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- excised shootse0
- Treatment
- Coronatine treatmente0
- Gene
- PR-1e0
- Outcome
- expression of a JA-regulated pathogenesis-related protein gene (PR-1)e0
- Direction
- did not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coronatine treatment of excised shoots caused increases in RNAs for jasmonic acid (JA)-regulated wound-response genes (LapA and pin2) but did not influence expression of a JA-regulated pathogenesis-related protein gene (PR-1)”
t0“Tomato”
Claim · model-written summary
The authors conclude that coronatine mimics a wound response but does not activate JA-regulated PR genes.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- coronatinee0
- Outcome
- wound responsee0
- Qualifier
- insufficient to activate JA-regulated PR genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coronatine mimicked the wound response but was insufficient to activate JA-regulated PR genes”
t0“Tomato”
The same questions in other papers
Coronatine · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.