Abstract
Upon delivery to the plant cell during infection, the Pseudomonas syringae effector protein AvrRpt2 undergoes proteolytic processing, enhances pathogen virulence and causes the elimination of the Arabidopsis RIN4 protein. A structure-prediction method was employed in order to investigate possible biochemical functions of AvrRpt2. Results of a secondary structure prediction algorithm suggest that the functional C-terminal portion of AvrRpt2 is a cysteine protease. Mutation of predicted catalytic residues within this portion of AvrRpt2 abolished in planta processing, elimination of Arabidopsis RIN4, and the ability to trigger an RPS2-specific resistance response. These data indicate that AvrRpt2 is most likely a sequence divergent cysteine protease whose activity is required for elimination of RIN4 during infection.
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Claims 5
Claim · model-written summary
Structure prediction suggested AvrRpt2 is a cysteine protease.
- Taxon
- Arabidopsis
Arabidopsise1 - Gene
- AvrRpt2e0
- Outcome
- cysteine proteasee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results of a secondary structure prediction algorithm suggest that the functional C-terminal portion of AvrRpt2 is a cysteine protease”
e1“Arabidopsis RIN4 protein”
Claim · model-written summary
Mutation of predicted AvrRpt2 catalytic residues abolished processing in planta.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Mutation of predicted catalytic residuese0
- Interactor
- AvrRpt2e0pathogen
- Gene
- AvrRpt2e0
- Outcome
- in planta processinge0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of predicted catalytic residues within this portion of AvrRpt2 abolished in planta processing, elimination of Arabidopsis RIN4, and the ability to trigger an RPS2-specific resistance response”
Claim · model-written summary
Mutation of AvrRpt2 catalytic residues abolished elimination of Arabidopsis RIN4.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Mutation of predicted catalytic residuese0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee1pathogen - Gene
- Arabidopsis RIN4e0
- Outcome
- elimination of Arabidopsis RIN4e0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of predicted catalytic residues within this portion of AvrRpt2 abolished in planta processing, elimination of Arabidopsis RIN4, and the ability to trigger an RPS2-specific resistance response”
e1“Pseudomonas syringae effector protein AvrRpt2”
Claim · model-written summary
Mutation of AvrRpt2 catalytic residues abolished the RPS2-specific resistance response.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Mutation of predicted catalytic residuese0
- Interactor
- Pseudomonas syringaePseudomonas syringaee1pathogen
- Outcome
- RPS2-specific resistance responsee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of predicted catalytic residues within this portion of AvrRpt2 abolished in planta processing, elimination of Arabidopsis RIN4, and the ability to trigger an RPS2-specific resistance response”
e1“Pseudomonas syringae effector protein AvrRpt2”
Claim · model-written summary
The authors infer that AvrRpt2 protease activity is required to eliminate RIN4 during infection.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- AvrRpt2e0
- Outcome
- elimination of RIN4e0
- Qualifier
- most likelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AvrRpt2 is most likely a sequence divergent cysteine protease whose activity is required for elimination of RIN4 during infection”
e1“Arabidopsis RIN4 protein”
The same questions in other papers
loss of function · Pseudomonas syringae · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 14 in the opposite direction
- no effect
- no effect
- negative
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.