Abstract
The effect of heavy metals on the modification of plasma membrane H(+)-ATPase (EC 3.6.3.14) activity in cucumber roots was studied. In plants stressed for 2 h with 10 microM or 100 microM Cd, Cu or Ni the hydrolytic as well as the transporting activity of H(+)-ATPase in the plasma membranes of root cells was decreased. Transcript levels of Cucumis sativus plasma membrane H(+)-ATPase in roots treated with 10 microM Cd, Cu, or Ni as well as with 100 microM Cu or Ni were similar to the control, indicating that the action of metals did not involve the gene expression level. Only in roots exposed to 100 microM Cd was the level of plasma membrane H(+)-ATPase mRNA markedly decreased. The inhibition of the plasma membrane proton pump caused by 100 microM Cd, Cu and Ni was partially diminished in the presence of cantharidin, a specific inhibitor of protein phosphatases. Western blot analysis with the antibody against phosphothreonine confirmed that decreased activity of plasma membrane H(+)-ATPase under heavy metals resulted from dephosphorylation of the enzyme protein. Taken together, these data strongly indicated that alteration of the enzyme under heavy metal stresses was mainly due to the post-translational modification of its proteins in short-term experiments.
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Claims 6
Claim · model-written summary
Cd, Cu and Ni stress decreased hydrolytic and transport activity of root H(+)-ATPase.
- Taxon
- cucumber
Cucumis sativuse1 - Organ / stage
- root cellse0
- Treatment
- 10 microM or 100 microM Cd, Cu or Nie0
- Gene
- H(+)-ATPasee0
- Outcome
- hydrolytic as well as the transporting activity of H(+)-ATPasee0
- Direction
- decreasede0
- Qualifier
- 2 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In plants stressed for 2 h with 10 microM or 100 microM Cd, Cu or Ni the hydrolytic as well as the transporting activity of H(+)-ATPase in the plasma membranes of root cells was decreased.”
e1“cucumber roots”
Claim · model-written summary
Most metal treatments left H(+)-ATPase transcript levels similar to control.
- Taxon
- Cucumis sativusCucumis sativuse0
- Organ / stage
- rootse0
- Treatment
- 10 microM Cd, Cu, or Ni as well as with 100 microM Cu or Nie0
- Gene
- plasma membrane H(+)-ATPasee0
- Outcome
- Transcript levelse0
- Direction
- similare0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript levels of Cucumis sativus plasma membrane H(+)-ATPase in roots treated with 10 microM Cd, Cu, or Ni as well as with 100 microM Cu or Ni were similar to the control”
Claim · model-written summary
At 100 microM Cd, H(+)-ATPase mRNA markedly decreased.
- Taxon
- Cucumis sativusCucumis sativust0
- Organ / stage
- rootse0
- Treatment
- 100 microM Cde0
- Gene
- plasma membrane H(+)-ATPasee0
- Outcome
- plasma membrane H(+)-ATPase mRNAe0
- Direction
- markedly decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only in roots exposed to 100 microM Cd was the level of plasma membrane H(+)-ATPase mRNA markedly decreased.”
t0“Cucumis sativus”
Claim · model-written summary
Cantharidin partially diminished metal inhibition of the proton pump.
- Taxon
- Cucumis sativusCucumis sativust0
- Treatment
- cantharidine0
- Outcome
- inhibition of the plasma membrane proton pumpe0
- Direction
- partially diminishede0
- Comparator
- 100 microM Cd, Cu and Nie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition of the plasma membrane proton pump caused by 100 microM Cd, Cu and Ni was partially diminished in the presence of cantharidin, a specific inhibitor of protein phosphatases.”
t0“Cucumis sativus”
Claim · model-written summary
Heavy metals decreased H(+)-ATPase activity through protein dephosphorylation.
- Taxon
- Cucumis sativusCucumis sativust0
- Treatment
- heavy metalse0
- Gene
- plasma membrane H(+)-ATPasee0
- Outcome
- decreased activity of plasma membrane H(+)-ATPasee0
- Qualifier
- dephosphorylation of the enzyme proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Western blot analysis with the antibody against phosphothreonine confirmed that decreased activity of plasma membrane H(+)-ATPase under heavy metals resulted from dephosphorylation of the enzyme protein.”
t0“Cucumis sativus”
Claim · model-written summary
Short term metal stress altered the enzyme mainly by post-translational modification.
- Taxon
- Cucumis sativusCucumis sativust0
- Treatment
- heavy metal stressese0
- Outcome
- alteration of the enzymee0
- Qualifier
- post-translational modification of its proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alteration of the enzyme under heavy metal stresses was mainly due to the post-translational modification of its proteins in short-term experiments.”
t0“Cucumis sativus”
The same questions in other papers
cadmium atom · molecular: expression · Cucumis sativus (cucumber)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
nickel atom · molecular: expression · Cucumis sativus (cucumber)Results agree
2 papers in the same direction as this paper
- negative
- no effect
copper atom · molecular: expression · Cucumis sativus (cucumber)Results agree
2 papers in the same direction as this paper
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.